scholarly journals Improved fidelity of thermostable ligases for detection of microsatellite repeat sequences using nucleoside analogs

1999 ◽  
Vol 27 (24) ◽  
pp. 41e-41 ◽  
Author(s):  
M Zirvi
Keyword(s):  
Nucleoside Analogs ◽  
Microsatellite Repeat ◽  
Repeat Sequences

scholarly journals Characterization of Duck Microsatellite Repeat Sequences

2001 ◽  
Vol 35 (4) ◽  
pp. 217-219 ◽  
Author(s):  
Hideaki TAKAHASHI ◽  
Masahiro SATOH ◽  
Mitsuru MINEZAWA ◽  
Tresnawati PURWADARIA ◽  
Hardi PRASETYO
Keyword(s):  
Microsatellite Repeat ◽  
Repeat Sequences

Isolation and characterisation of microsatellite loci in the bush stone-curlew (Burhinus grallarius), a declining Australian bird

2013 ◽  
Vol 61 (6) ◽  
pp. 421
Author(s):  
Robert A. B. Mason ◽  
Catherine Price ◽  
Walter E. Boles ◽  
Karen-Anne Gray ◽  
Edwina Rickard ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Allelic Diversity ◽  
Pcr Amplification ◽  
Habitat Modification ◽  
Microsatellite Repeat ◽  
Gene Encoding ◽  
Repeat Sequences ◽  
Introduced Predators ◽  
Ground Nesting

The bush stone-curlew (Burhinus grallarius Latham), a ground-nesting nocturnal bird, is endangered in southern Australia due to habitat modification and introduced predators. To provide tools for conservation, ecological and behavioural studies, we isolated variable microsatellite repeat sequences and designed primers for PCR amplification in this species. Primer pairs were developed and levels of diversity were assessed for eight microsatellite loci, including one locus linked to the gene encoding Microtubule-Associated Protein 2, a protein important for behavioural imprinting in birds, and one sex-linked locus. Isolated loci contained allelic diversity of between 5 and 17 alleles.

A small-insert bovine genomic library highly enriched for microsatellite repeat sequences

1995 ◽  
Vol 6 (10) ◽  
pp. 714-724 ◽  
Author(s):  
R. T. Stone ◽  
J. C. Pulido ◽  
G. M. Duyk ◽  
S. M. Kappes ◽  
J. W. Keele ◽  
...  
Keyword(s):  
Genomic Library ◽  
Microsatellite Repeat ◽  
Repeat Sequences

scholarly journals An Effcient Method to Clone Chicken Microsatellite Repeat Sequences.

1996 ◽  
Vol 33 (5) ◽  
pp. 292-299 ◽  
Author(s):  
Hideaki Takahashi ◽  
Keijiro Nirasawa ◽  
Tsutomu Furukawa
Keyword(s):  
Microsatellite Repeat ◽  
Repeat Sequences

scholarly journals Characterization and application of fluidic properties of trinucleotide repeat sequences by wax-on-plastic microfluidics

2020 ◽  
Vol 8 (4) ◽  
pp. 743-751 ◽  
Author(s):  
Ahmad Zaman Qamar ◽  
Narges Asefifeyzabadi ◽  
Motahareh Taki ◽  
Swati Naphade ◽  
Lisa M. Ellerby ◽  
...  
Keyword(s):  
Trinucleotide Repeat ◽  
Capillary Flow ◽  
Microsatellite Repeat ◽  
Repeat Sequences ◽  
Repeat Expansions ◽  
Plastic Microfluidics

This work demonstrates sequence directed flexibility of neurodegenerative trinucleotide repeat sequences can be translated into distinct capillary flow using wax-on-plastic microfluidics and used to detect microsatellite repeat expansions diseases.

Precision length determination and in silico simulation in PCR of microsatellite repeat sequences

2021 ◽  
Author(s):  
Seijiro Shioi ◽  
Akiyoshi Shimamoto ◽  
Yuki Nakagami ◽  
Lexin Qin ◽  
Mototsugu Shimokawa ◽  
...  
Keyword(s):  
In Silico ◽  
Microsatellite Repeat ◽  
Repeat Sequences ◽  
In Silico Simulation ◽  
Length Determination

scholarly journals Impeding transcription of expanded microsatellite repeats by deactivated Cas9

2017 ◽  
Author(s):  
Belinda S. Pinto ◽  
Tanvi Saxena ◽  
Ruan Oliveira ◽  
Héctor R. Méndez-Gómez ◽  
John D. Cleary ◽  
...  
Keyword(s):  
Corneal Dystrophy ◽  
Therapeutic Benefit ◽  
Systemic Delivery ◽  
Microsatellite Repeat ◽  
Aberrant Splicing ◽  
Adeno Associated Virus ◽  
Microsatellite Repeats ◽  
Repeat Sequences ◽  
Rna Foci ◽  
Splicing Patterns

SummaryTranscription of expanded microsatellite repeats is associated with multiple human diseases, including myotonic dystrophy, Fuchs’ endothelial corneal dystrophy, andC9orf72-ALS/FTD. Eliminating or reducing production of RNA and proteins arising from these expanded loci holds therapeutic benefit. Here, we tested the hypothesis that a deactivated form of the Cas9 enzyme impedes transcription across expanded microsatellites. We observed a repeat length-, PAM-, and strand-dependent reduction in the abundance of repeat-containing RNAs upon targeting dCas9 directly to repeat sequences. Aberrant splicing patterns were rescued in DM1 cells, and production of RAN peptides characteristic of DM1, DM2, andC9orf72-ALS/FTD cells was drastically decreased. Systemic delivery of dCas9/gRNA by adeno-associated virus led to reductions in pathological RNA foci, rescue of chloride channel 1 protein expression, and decreased myotonia. These observations suggest that transcription of microsatellite repeat-containing RNAs is more sensitive to perturbation than transcription of other RNAs, indicating potentially viable strategies for therapeutic intervention.

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