scholarly journals RAV1, a novel DNA-binding protein, binds to bipartite recognition sequence through two distinct DNA-binding domains uniquely found in higher plants

1999 ◽  
Vol 27 (2) ◽  
pp. 470-478 ◽  
Author(s):  
Y. Kagaya ◽  
K. Ohmiya ◽  
T. Hattori
Keyword(s):  
Dna Binding ◽  
Higher Plants ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Recognition Sequence ◽  
Dna Binding Domains ◽  
Binding Domains

scholarly journals Rhodopsin targeted transcriptional silencing by DNA-binding

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Salvatore Botta ◽  
Elena Marrocco ◽  
Nicola de Prisco ◽  
Fabiola Curion ◽  
Mario Renda ◽  
...  
Keyword(s):  
Dna Binding ◽  
Transcriptional Repression ◽  
Binding Protein ◽  
Regulatory Element ◽  
Transcriptional Silencing ◽  
Dna Binding Protein ◽  
Gain Of Function ◽  
Dna Binding Domains ◽  
Binding Domains

Transcription factors (TFs) operate by the combined activity of their DNA-binding domains (DBDs) and effector domains (EDs) enabling the coordination of gene expression on a genomic scale. Here we show that in vivo delivery of an engineered DNA-binding protein uncoupled from the repressor domain can produce efficient and gene-specific transcriptional silencing. To interfere with RHODOPSIN (RHO) gain-of-function mutations we engineered the ZF6-DNA-binding protein (ZF6-DB) that targets 20 base pairs (bp) of a RHOcis-regulatory element (CRE) and demonstrate Rho specific transcriptional silencing upon adeno-associated viral (AAV) vector-mediated expression in photoreceptors. The data show that the 20 bp-long genomic DNA sequence is necessary for RHO expression and that photoreceptor delivery of the corresponding cognate synthetic trans-acting factor ZF6-DB without the intrinsic transcriptional repression properties of the canonical ED blocks Rho expression with negligible genome-wide transcript perturbations. The data support DNA-binding-mediated silencing as a novel mode to treat gain-of-function mutations.

scholarly journals Encoded, click-reactive DNA-binding domains for programmable capture of specific chromatin segments

2020 ◽  
Vol 11 (46) ◽  
pp. 12506-12511
Author(s):  
Anna Witte ◽  
Álvaro Muñoz-López ◽  
Malte Metz ◽  
Michal R. Schweiger ◽  
Petra Janning ◽  
...  
Keyword(s):  
Amino Acids ◽  
Dna Binding ◽  
Binding Protein ◽  
Protein Domains ◽  
Dna Binding Protein ◽  
Noncanonical Amino Acids ◽  
Dna Binding Domains ◽  
Binding Domains

We report programmable DNA-binding protein domains bearing genetically encoded noncanonical amino acids for click-biotinylation and enrichment of bound chromatin segments from cells for downstream analyses.

scholarly journals Recognition sequence of a highly conserved DNA binding protein RBP-Jx

1994 ◽  
Vol 22 (6) ◽  
pp. 965-971 ◽  
Author(s):  
Tin Tun ◽  
Yasushi Hamaguchi ◽  
Norisada Matsunami ◽  
Takahisa Furukawa ◽  
Tasuku Honjo ◽  
...  
Keyword(s):  
Dna Binding ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Recognition Sequence
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