scholarly journals Site-specific targeting of psoralen photoadducts with a triple helix-forming oligonucleotide: characterization of psoralen monoadduct and crosslink formation

1994 ◽  
Vol 22 (14) ◽  
pp. 2845-2852 ◽  
Author(s):  
Francis P. Gasparro ◽  
Pamela A. Havre ◽  
Gerard A. Olack ◽  
Edward J. Gunther ◽  
Peter M. Glazer
Keyword(s):  
Triple Helix ◽  
Site Specific ◽  
Specific Targeting

Gelatin and Glycerine-Based Bioadhesive Vaginal Hydrogel

2020 ◽  
Vol 17 (4) ◽  
pp. 303-311
Author(s):  
Roberta Cassano ◽  
Federica Curcio ◽  
Delia Mandracchia ◽  
Adriana Trapani ◽  
Sonia Trombino
Keyword(s):  
Differential Scanning Calorimetry ◽  
Inflammatory Process ◽  
Vaginal Mucosa ◽  
Vaginal Infection ◽  
Scanning Calorimetry ◽  
Time Intervals ◽  
Site Specific ◽  
Release Studies ◽  
Hiv Seropositive

Aim: The work’s aim was the preparation and characterization of a hydrogel based on gelatin and glycerine, useful for site-specific release of benzydamine, an anti-inflammatory drug, able to attenuate the inflammatory process typical of the vaginal infection. Objective: The obtained hydrogel has been characterized by Electronic Scanning Microscopy (SEM) and Differential Scanning Calorimetry (DSC). In addition, due to the precursor properties, the hydrogel exhibits a relevant mucoadhesive activity. Methods: The swelling degree was evaluated at two different pHs and at defined time intervals. In particular, phosphate buffers were used at pH 6.6, in order to mimic the typical conditions of infectious diseases at the vaginal level, particularly for HIV-seropositive pregnant women, and pH 4.6, to simulate the physiological environment. Results: The obtained results revealed that the hydrogel swells up well at both pHs. Conclusion: Release studies conducted at both pathological and physiological pHs have shown that benzydamine is released at the level of the vaginal mucosa in a slow and gradual manner. These data support the hypothesis of the hydrogel use for the site-specific release of benzydamine in the vaginal mucosa.

scholarly journals Butyrophilin-like 2 regulates site-specific adaptations of intestinal γδ intraepithelial lymphocytes

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Casandra Panea ◽  
Ruoyu Zhang ◽  
Jeffrey VanValkenburgh ◽  
Min Ni ◽  
Christina Adler ◽  
...  
Keyword(s):  
Immune Responses ◽  
Intraepithelial Lymphocytes ◽  
Homeostatic Proliferation ◽  
Intestinal Epithelial ◽  
Intestinal Epithelial Barrier ◽  
Site Specific ◽  
Adaptive Immune ◽  
Intestinal Segments ◽  
Repertoire Diversity

AbstractTissue-resident γδ intraepithelial lymphocytes (IELs) orchestrate innate and adaptive immune responses to maintain intestinal epithelial barrier integrity. Epithelia-specific butyrophilin-like (Btnl) molecules induce perinatal development of distinct Vγ TCR+ IELs, however, the mechanisms that control γδ IEL maintenance within discrete intestinal segments are unclear. Here, we show that Btnl2 suppressed homeostatic proliferation of γδ IELs preferentially in the ileum. High throughput transcriptomic characterization of site-specific Btnl2-KO γδ IELs reveals that Btnl2 regulated the antimicrobial response module of ileal γδ IELs. Btnl2 deficiency shapes the TCR specificities and TCRγ/δ repertoire diversity of ileal γδ IELs. During DSS-induced colitis, Btnl2-KO mice exhibit increased inflammation and delayed mucosal repair in the colon. Collectively, these data suggest that Btnl2 fine-tunes γδ IEL frequencies and TCR specificities in response to site-specific homeostatic and inflammatory cues. Hence, Btnl-mediated targeting of γδ IEL development and maintenance may help dissect their immunological functions in intestinal diseases with segment-specific manifestations.

scholarly journals Site-specific inhibition ofEcoRI restriction/modification enzymes by a DNA triple helix

1990 ◽  
Vol 18 (1) ◽  
pp. 157-161 ◽  
Author(s):  
Jeffery C. Hanvey ◽  
Mitsuhiro Shimizu ◽  
Robert D. Wells
Keyword(s):  
Triple Helix ◽  
Specific Inhibition ◽  
Site Specific ◽  
Dna Triple Helix ◽  
Restriction Modification
Sign in / Sign up

Export Citation Format

Share Document