scholarly journals Lead cleavage sites in the core structure of group I intron-RNA

1993 ◽  
Vol 21 (2) ◽  
pp. 311-317 ◽  
Author(s):  
Barbara Striecjer ◽  
Uwe von Ahsen ◽  
Renée Schroeder
Keyword(s):  
Group I Intron ◽  
Core Structure ◽  
Cleavage Sites ◽  
The Core ◽  
Group I

Important 2'-hydroxyl groups within the core of a group I intron

Biochemistry ◽  
1993 ◽  
Vol 32 (14) ◽  
pp. 3604-3610 ◽  
Author(s):  
Mark G. Caprara ◽  
Richard B. Waring
Keyword(s):  
Group I Intron ◽  
Hydroxyl Groups ◽  
The Core ◽  
Group I

scholarly journals Sub-3 Angstrom cryo-EM structure of RNA enabled by engineered homomeric self-assembly

2021 ◽  
Author(s):  
Di Liu ◽  
Francois A. Thelot ◽  
Joseph A. Piccirilli ◽  
Maofu Liao ◽  
Peng Yin
Keyword(s):  
Self Assembly ◽  
Model Building ◽  
De Novo ◽  
Group I Intron ◽  
Structural Flexibility ◽  
Structural Studies ◽  
The Core ◽  
Group I ◽  
Construct Optimization ◽  
Functional Rnas

Many functional RNAs fold into intricate and precise 3D architectures, and high-resolution structures are required to understand their underlying mechanistic principles. However, RNA structural determination is difficult. Herein, we present a nanoarchitectural strategy to enable the efficient single-particle cryogenic electron microscopy (cryo-EM) analysis of RNA-only structures. This strategy, termed RNA oligomerization-enabled cryo-EM via installing kissing-loops (ROCK), involves the engineering of target RNAs by installing kissing-loop sequences onto functionally nonessential stems for the assembly into closed homomeric nanoarchitectures. Assembly with geometric restraints leads to (1) molecular weight multiplication and (2) structural flexibility mitigation, both beneficial for cryo-EM analysis. Together with construct optimization and symmetry-expansion reconstruction, ROCK yields the cryo-EM reconstruction of the Tetrahymena group I intron at an overall resolution of 2.98 Angstrom (2.85 Angstrom resolution for the core domains), enabling the de novo model building of the complete intron RNA including previously unknown peripheral domains. When applied to smaller RNAs, ROCK readily produces modest-resolution maps, revealing the conformational rearrangement of the Azoarcus group I intron and the bound ligand in the FMN riboswitch. Our work unleashes the largely unexplored potential of cryo-EM in RNA structural studies.

Indexicality of Grammar: The Case of Japanese Transgender Speakers

2019 ◽  
Author(s):  
Hideko Abe
Keyword(s):  
Gender Identity ◽  
Gender Identity Disorder ◽  
Grammatical Gender ◽  
Core Structure ◽  
Subject Positions ◽  
Transgender Identity ◽  
The Core ◽  
History Of ◽  
History Of Scholarship ◽  
Grammatical Structures

This article discusses how the intersection of grammatical gender and social gender, entwined in the core structure of language, can be analyzed to understand the dynamic status of selfhood. After reviewing a history of scholarship that demonstrates this claim, the discussion analyzes the language practices of transgender individuals in Japan, where transgender identity is currently understood in terms of sei-dōitsusei-shōgai (gender identity disorder). Based on fieldwork conducted between 2011 and 2017, the analysis reveals how individuals identifying with sei-dōitsusei-shōgai negotiate subject positions by manipulating the specific indexical meanings attached to grammatical structures.

Solvent-free and efficient synthesis of bicyclic 2-pyridone derivatives for endoplasmic reticulum imaging

2021 ◽  
Author(s):  
Xiaoyun Ran ◽  
Qian Zhou ◽  
Jin Zhang ◽  
Shanqiang Wang ◽  
Gui Wang ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Citric Acid ◽  
Core Structure ◽  
Solvent Free ◽  
Efficient Synthesis ◽  
The Core ◽  
Catalyst Free ◽  
Synthesis Approach ◽  
Ethylenediamine Derivatives

Started from citric acid (CA) and ethylenediamine derivatives, a solvent-free, catalyst-free and highly yield synthesis approach for bicyclic 2-pyridones was presented. Continuing to modify the core structure, a series of...

scholarly journals The Core Structure and Immunological Activities of Glycyrrhizan UA, the Main Polysaccharide from the Root of Glycyrrhiza uralensis.

1992 ◽  
Vol 40 (8) ◽  
pp. 2125-2128 ◽  
Author(s):  
Noriko SHIMIZU ◽  
Masashi TOMODA ◽  
Katsutoshi TAKADA ◽  
Ryoko GONDA
Keyword(s):  
Core Structure ◽  
Glycyrrhiza Uralensis ◽  
The Core

scholarly journals Evaluation of Aberration-corrected Optical Sectioning for Exploring the Core Structure of ½[111] Screw Dislocations in BCC Metals

2017 ◽  
Vol 23 (S1) ◽  
pp. 432-433
Author(s):  
D. Hernandez-Maldonado ◽  
R. Groger ◽  
Q. M. Ramasse ◽  
P. B. Hirsch ◽  
P.D. Nellist
Keyword(s):  
Core Structure ◽  
Optical Sectioning ◽  
Screw Dislocations ◽  
The Core ◽  
Bcc Metals ◽  
Aberration Corrected
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