scholarly journals An additional ribosome-binding site on mRNA of highly expressed genes and a bifunctional site on the colicin fragment of 16S rRNA fromEscherichia coli: important determinants of the efficiency of translation-initiation

1989 ◽  
Vol 17 (8) ◽  
pp. 2973-2985 ◽  
Author(s):  
T.A. Thanaraj ◽  
M.W. Pandit
Keyword(s):  
16S Rrna ◽  
Binding Site ◽  
Translation Initiation ◽  
Ribosome Binding Site ◽  
Ribosome Binding ◽  
Fromescherichia Coli ◽  
Highly Expressed Genes

Translation initiation in Escherichia coli: sequences within the ribosome-binding site

1992 ◽  
Vol 6 (9) ◽  
pp. 1219-1229 ◽  
Author(s):  
Steven Ringquist ◽  
Sidney Shinedling ◽  
Doug Barrick ◽  
Louis Green ◽  
Jonathan Binkley ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Binding Site ◽  
Translation Initiation ◽  
Ribosome Binding Site ◽  
Ribosome Binding

pGR71 plasmid promotor sequence temporally regulated in Bacillus subtilis

1998 ◽  
Vol 44 (12) ◽  
pp. 1186-1192
Author(s):  
Guy Daxhelet ◽  
Philippe Gilot ◽  
Etienne Nyssen ◽  
Philippe Hoet
Keyword(s):  
Bacillus Subtilis ◽  
Binding Site ◽  
Transcription Initiation ◽  
Promoter Sequence ◽  
Initiation Site ◽  
Chloramphenicol Acetyltransferase ◽  
Ribosome Binding Site ◽  
Chloramphenicol Resistance ◽  
E Coli ◽  
Ribosome Binding

pGR71, a composite of plasmids pUB110 and pBR322, replicates in Escherichia coli and in Bacillus subtilis. It carries the chloramphenicol resistance gene (cat) from Tn9, which is not transcribed in either host by lack of a promoter. The cat gene is preceded by a Shine-Dalgarno sequence functional in E. coli but not in B. subtilis. Deleted pGR71 plasmids were obtained in B. subtilis when cloning foreign viral DNA upstream of this cat sequence, as well as by BAL31 exonuclease deletions extending upstream from the cat into the pUB110 moiety. These mutant plasmids expressed chloramphenicol acetyltransferase (CAT), conferring on B. subtilis resistance to high chloramphenicol concentrations. CAT expression peaked at the early postexponential phase of B. subtilis growth. The transcription initiation site of cat, determined by primer extension, was located downstream of a putative promoter sequence within the pUB110 moiety. N-terminal amino acid sequencing showed that native CAT was produced by these mutant plasmids. The cat ribosome-binding site, functional in E. coli, was repositioned within the pUB110 moiety and had consequently an extended homology with B. subtilis 16S rRNA, explaining the production of native enzyme.Key words: chloramphenicol acetyltransferase, Bacillus subtilis, postexponential gene expression, plasmid pUB110, ribosome-binding site, transcriptional promoter.

Small molecules targeting ribosome binding site of ricin toxin a subunit

Toxicon ◽  
2020 ◽  
Vol 177 ◽  
pp. S45
Author(s):  
Xiao-Ping Li ◽  
Nilgun E. Tumer ◽  
Jennifer Nielsen Kahn
Keyword(s):  
Small Molecules ◽  
Binding Site ◽  
Ribosome Binding Site ◽  
Toxin A ◽  
Ricin Toxin ◽  
Ribosome Binding ◽  
A Subunit

scholarly journals Spanning high-dimensional expression space using ribosome-binding site combinatorics

2013 ◽  
Vol 41 (9) ◽  
pp. e98-e98 ◽  
Author(s):  
Lior Zelcbuch ◽  
Niv Antonovsky ◽  
Arren Bar-Even ◽  
Ayelet Levin-Karp ◽  
Uri Barenholz ◽  
...  
Keyword(s):  
Binding Site ◽  
Ribosome Binding Site ◽  
High Dimensional ◽  
Ribosome Binding

Mapping the lacZ ribosome binding site by RNA footprinting

Biochemistry ◽  
1989 ◽  
Vol 28 (20) ◽  
pp. 8067-8072 ◽  
Author(s):  
George J. Murakawa ◽  
Donald P. Nierlich
Keyword(s):  
Binding Site ◽  
Ribosome Binding Site ◽  
Ribosome Binding
Sign in / Sign up

Export Citation Format

Share Document