scholarly journals Characteristic alteration in the nuclear DNA polymerase activity during the cell division cycle ofSaccharomyces cerevisiae

1984 ◽  
Vol 12 (7) ◽  
pp. 3143-3154 ◽  
Author(s):  
Eiko Tsuchiya ◽  
Kosei Kimura ◽  
Tokichi Miyakawa ◽  
Sakuzo Fukui
Keyword(s):  
Cell Division ◽  
Dna Polymerase ◽  
Nuclear Dna ◽  
Polymerase Activity ◽  
Cell Division Cycle

DNA Polymerase in Avian Erythroid Cells

1972 ◽  
Vol 11 (3) ◽  
pp. 785-798
Author(s):  
A. F. WILLIAMS
Keyword(s):  
Cell Division ◽  
Dna Synthesis ◽  
Dna Polymerase ◽  
Cell Types ◽  
Polymerase Activity ◽  
Erythroid Cells ◽  
Isolated Nuclei ◽  
Dividing Cells ◽  
Different Cell Types

The level and properties of DNA polymerase activity assayable in extracts of avian erythroid cells was studied. The enzyme was detectable in the dividing cells (erythroblasts) of the erythropoietic series and also the immature non-dividing erythrocytes. It could not be assayed in mature erythrocytes. Investigations showed that activity began to decline at the time of the last cell division of the erythroid series. Properties of the enzyme did change in different cell types; however, the changes did not correlate with cessation of DNA synthesis. Some preliminary results on DNA synthesis by isolated nuclei are also reported and these showed that only nuclei from erythroblasts could synthesize DNA in vitro in the absence of primer.

scholarly journals Presence of two deoxyribonucleic acid polymerases in bull spermatozoa

1978 ◽  
Vol 175 (2) ◽  
pp. 595-600 ◽  
Author(s):  
M Philippe ◽  
P Chevaillier
Keyword(s):  
Ionic Strength ◽  
Dna Polymerase ◽  
Ethidium Bromide ◽  
Nuclear Dna ◽  
Buoyant Density ◽  
Sedimentation Coefficient ◽  
Polymerase Activity ◽  
Exogenous Dna ◽  
Middle Piece ◽  
Low Ionic Strength

A DNA polymerase-endogenous template complex was isolated from nuclear heads of bull spermatozoa. The buoyant density of the complex was 1.15 g/cm 3. The sedimentation coefficient of the nuclear DNA polymerase isolated from the complex was higher at low ionic strength, but approached 3.4S when centrifuged in a medium containing 2M-KCl. Activated exogenous DNA increased polymerase activity. Only very low activities were detected with synthetic templates such as poly(A).(dT)12-18 and poly(dT).poly(A). The nuclear reaction was stimulated by 150mM-KCl and was slightly inhibited by N-ethylmaleimide; it was resistant to actinomycin D, netropsin and ethidium bromide. Another DNA polymerase, highly sensitive to ethidium bromide, was extracted from the mitochondira-rich middle-piece fraction. Its sedimentation coefficient was close to 9S, but fell to approx. 4S in high-ionic-strength medium.

XV.—The metabolism of DNA in the liver during precocious induction of metamorphosis in Rana catesbeiana

1969 ◽  
Vol 70 (4) ◽  
pp. 295-310 ◽  
Author(s):  
Ailsa M. Campbell ◽  
Martita H. Corrance ◽  
J. N. Davidson ◽  
H. M. Keir
Keyword(s):  
Dna Polymerase ◽  
Nuclear Dna ◽  
Rana Catesbeiana ◽  
Specific Activity ◽  
Mitochondrial Fraction ◽  
Polymerase Activity ◽  
Nucleic Acid Metabolism ◽  
Cell Extracts

SynopsisStudies have been made on the incorporation of [8H]-deoxythymidine into the DNA of the livers of Rana catesbeiana tadpoles. When metamorphosis was induced with tri-iodothyronine, the specific activity of the nuclear DNA rose 5 days after administration of the hormone. In contrast, the specific activity of the DNA from the mitochondrial fraction rose grave–2 days after hormone administration.In order to determine whether the in vivo change was due to alterations in the pool sizes of the DNA precursors, in vitro studies on DNA polymerase were carried out. It was found that under conditions where the enzyme activity was not limited by availability of template or substrates, there was a rise in the DNA polymerase activity in crude cell extracts from the tadpole liver. Fractionation of the cell components showed that little of this increment in activity appeared to be located in the nucleus, but that a large percentage alteration in activity occurred in the mitochondrial and cell sap fractions.A possible interpretation of these results is that an increase in the mitochondrial DNA polymerase is one of the early effects of thyroid hormone. This possibility is discussed in relation to the other known effects of thyroid hormones in tadpoles, with particular reference to nucleic acid metabolism and also to mitochondrial hyperplasia.

Regulation of genes for the cdc25 phosphatases is important for checkpoint control during the cell division cycle

2001 ◽  
Vol 120 (5) ◽  
pp. A501-A501
Author(s):  
U HAUGWITZ ◽  
M WIEDMANN ◽  
K SPIESBACH ◽  
K ENGELAND ◽  
J MOSSNER
Keyword(s):  
Cell Division ◽  
Cell Division Cycle ◽  
Checkpoint Control

PrimPol (Primase/Polymerase), replicating enzyme – A mini review

2020 ◽  
Vol 2 (4) ◽  
pp. 89-92
Author(s):  
Muhammad Amir ◽  
Sabeera Afzal ◽  
Alia Ishaq
Keyword(s):  
Dna Replication ◽  
Dna Polymerase ◽  
Genetic Information ◽  
Nuclear Dna ◽  
De Novo ◽  
Dna Polymerases ◽  
Test Site ◽  
Mitochondrial Dna Replication ◽  
Dna Template ◽  
Preliminary Phase

Polymerases were revealed first in 1970s. Most important to the modest perception the enzyme responsible for nuclear DNA replication that was pol , for DNA repair pol and for mitochondrial DNA replication pol  DNA construction and renovation done by DNA polymerases, so directing both the constancy and discrepancy of genetic information. Replication of genome initiate with DNA template-dependent fusion of small primers of RNA. This preliminary phase in replication of DNA demarcated as de novo primer synthesis which is catalyzed by specified polymerases known as primases. Sixteen diverse DNA-synthesizing enzymes about human perspective are devoted to replication, reparation, mutilation lenience, and inconsistency of nuclear DNA. But in dissimilarity, merely one DNA polymerase has been called in mitochondria. It has been suggest that PrimPol is extremely acting the roles by re-priming DNA replication in mitochondria to permit an effective and appropriate way replication to be accomplished. Investigations from a numeral of test site have significantly amplified our appreciative of the role, recruitment and regulation of the enzyme during DNA replication. Though, we are simply just start to increase in value the versatile roles that play PrimPol in eukaryote.

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