scholarly journals Reduced-stringency DNA reassociation: sequence specific duplex formation

1982 ◽  
Vol 10 (2) ◽  
pp. 719-733 ◽  
Author(s):  
Henry E. Burr ◽  
Robert T. Schimke
Keyword(s):  
Dna Reassociation ◽  
Duplex Formation

Genetic homologies among nocardiae and other actinomycetes

1973 ◽  
Vol 19 (8) ◽  
pp. 1007-1014 ◽  
Author(s):  
S. G. Bradley ◽  
G. H. Brownell ◽  
Josephine Clark
Keyword(s):  
Dna Hybridization ◽  
Genetic Recombination ◽  
Nucleotide Sequences ◽  
Dna Reassociation ◽  
Hybrid Molecules ◽  
Nearly Neutral Mutations ◽  
Neutral Mutations ◽  
Incubation Temperatures ◽  
Duplex Formation ◽  
Discrete Populations

The extent of association between deoxyribonucleic acid (DNA) from various actinomycetes with reference DNA samples from two strains of Nocardia erythropolis has been used as an index of relatedness. N. erythropolis, N. canicruria, and particular cultures designated as N. corallina and N. opaca constitute a genospecies, on the bases of ability to form DNA hybrid molecules and ability to participate in genetic recombination. The lack of reciprocity in the DNA reassociation assays involving selected strains of N. erythropolis indicates that the DNA from particular lineages possesses unique nucleotide sequences. The taxon Mycobacterium rhodochrous is heterogeneous and contains at least two discrete populations. The present results do not clarify the relationships of the N. erythropolis genospecies to the other nocardiae, streptomycetes, or mycobacteria. The differences in extent of hybrid DNA duplex formation at exacting and non-exacting incubation temperatures reflect the degree of matching between the compared nucleotide sequences. On this basis, actinomycete DNA samples differ from one another by nucleotide alterations dispersed throughout the genome. DNA hybridization is a sensitive assay for molecular evolution and may be able to detect neutral and nearly neutral mutations that are not recognized phenotypically.

Cell Wall Ultrastructure in Three Strains of a Cariogenic Streptococcus

1971 ◽  
Vol 29 ◽  
pp. 338-339
Author(s):  
M. J. Kramer ◽  
Alan L. Coykendall
Keyword(s):  
Cell Wall ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Genetic Heterogeneity ◽  
Morphological Characteristics ◽  
Electron Microscopic ◽  
Dna Reassociation ◽  
Wall Ultrastructure

During the almost 50 years since Streptococcus mutans was first suggested as a factor in the etiology of dental caries, a multitude of studies have confirmed the cariogenic potential of this organism. Streptococci have been isolated from human and animal caries on numerous occasions and, with few exceptions, they are not typable by the Lancefield technique but are relatively homogeneous in their biochemical reactions. An analysis of the guanine-cytosine (G-C) composition of the DNA from strains K-1-R, NCTC 10449, and FA-1 by one of us (ALC) revealed significant differences and DNA-DNA reassociation experiments indicated that genetic heterogeneity existed among the three strains. The present electron microscopic study had as its objective the elucidation of any distinguishing morphological characteristics which might further characterize the respective strains.

scholarly journals Xenopus transcription factor A promotes DNA reassociation.

1985 ◽  
Vol 260 (24) ◽  
pp. 13316-13320 ◽  
Author(s):  
J S Hanas ◽  
D J Hazuda ◽  
C W Wu
Keyword(s):  
Transcription Factor ◽  
Dna Reassociation

Kinetics and Thermodynamics of DNA, RNA, and Hybrid Duplex Formation

Biochemistry ◽  
2013 ◽  
Vol 52 (5) ◽  
pp. 765-772 ◽  
Author(s):  
Brittany Rauzan ◽  
Elizabeth McMichael ◽  
Rachel Cave ◽  
Lesley R. Sevcik ◽  
Kara Ostrosky ◽  
...  
Keyword(s):  
Kinetics And Thermodynamics ◽  
Hybrid Duplex ◽  
Duplex Formation

Successive Fusion of Vesicles Aggregated by DNA Duplex Formation in the Presence of Triton X-100

2008 ◽  
Vol 37 (3) ◽  
pp. 340-341 ◽  
Author(s):  
Naoto Maru ◽  
Koh-ichiroh Shohda ◽  
Tadashi Sugawara
Keyword(s):  
Dna Duplex ◽  
Triton X ◽  
Duplex Formation

Spectral and cyclic voltammetric characterization of self-assembled monolayers on gold of pyrene end-labeled oligonucleotide duplexes

1999 ◽  
Vol 77 (5-6) ◽  
pp. 1077-1084 ◽  
Author(s):  
R Scott Reese ◽  
Marye Anne Fox
Keyword(s):  
Gold Surface ◽  
Self Assembled Monolayers ◽  
Self Assembled Monolayer ◽  
Surface Emission ◽  
Assembled Monolayers ◽  
Oligonucleotide Duplex ◽  
Self Assembled ◽  
Duplex Formation

Self-assembled monolayers of sulfur-terminated oligonucleotide duplexes were formed on flat gold surfaces, either by exposure of a self-assembled monolayer bearing one oligonucleotide strand to the complementary strand or by preformation of a oligonucleotide duplex that was then deposited on a fresh gold surface. Virtually identical spectral behavior was observed whether the duplex was produced before deposition or by in situ complementary association. With a duplex bearing an appropriate pyrene end-label, the resulting thin film was photoresponsive. Surface emission measurements show no evidence for pyrene aggregation on the modified surfaces. The polarity of the photocurrent, reflecting photoinduced electron transfer initiated by photoexcitation of pyrene, is opposite that expected from the oligonucleotide-mediated reduction of the appended pyrene excited state.Key words: oligonucleotide, self-assembled monolayer, duplex formation, photoelectrochemistry, surface emission.

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