scholarly journals Expression and promoter methylation status of two DNA repair genes in leukocytes from patients undergoing propofol or isoflurane anaesthesia

Mutagenesis ◽  
2018 ◽  
Vol 33 (2) ◽  
pp. 147-152 ◽  
Author(s):  
Cristiana M M Freire ◽  
Mariana G Braz ◽  
João Paulo C Marcondes ◽  
Nayara M Arruda ◽  
José Reinaldo C Braz ◽  
...  
Keyword(s):  
Dna Repair ◽  
Promoter Methylation ◽  
Methylation Status ◽  
Dna Repair Genes ◽  
Isoflurane Anaesthesia ◽  
Promoter Methylation Status ◽  
Repair Genes

Promoter Methylation Status of the DNA Repair Genes hMLH1 and MGMT in Gastric Carcinoma and Metaplastic Mucosa

Pathobiology ◽  
2001 ◽  
Vol 69 (3) ◽  
pp. 143-149 ◽  
Author(s):  
Naohide Oue ◽  
Kazuhiro Sentani ◽  
Hiroshi Yokozaki ◽  
Yasuhiko Kitadai ◽  
Reiko Ito ◽  
...  
Keyword(s):  
Dna Repair ◽  
Gastric Carcinoma ◽  
Promoter Methylation ◽  
Methylation Status ◽  
Dna Repair Genes ◽  
Promoter Methylation Status ◽  
Repair Genes

scholarly journals Lead Induces Genotoxicity via Oxidative Stress and Promoter Methylation of DNA Repair Genes in Human Lymphoblastoid TK6 Cells

2018 ◽  
Vol 24 ◽  
pp. 4295-4304 ◽  
Author(s):  
Xiangquan Liu ◽  
Jingying Wu ◽  
Wenyan Shi ◽  
Wenhua Shi ◽  
Hekun Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Repair ◽  
Promoter Methylation ◽  
Dna Repair Genes ◽  
Methylation Of Dna ◽  
Tk6 Cells ◽  
Repair Genes

Promoter methylation and expression of DNA repair genes MGMT and ERCC1 in tissue and blood of rectal cancer patients

Gene ◽  
2018 ◽  
Vol 644 ◽  
pp. 66-73 ◽  
Author(s):  
Sally M. Shalaby ◽  
Amal S. El-Shal ◽  
Lobna A. Abdelaziz ◽  
Eman Abd-Elbary ◽  
Mostafa M. Khairy
Keyword(s):  
Rectal Cancer ◽  
Dna Repair ◽  
Cancer Patients ◽  
Promoter Methylation ◽  
Dna Repair Genes ◽  
Repair Genes

scholarly journals Placental promoter methylation of DNA repair genes and prenatal exposure to particulate air pollution: an ENVIR ON AGE cohort study

2018 ◽  
Vol 2 (4) ◽  
pp. e174-e183 ◽  
Author(s):  
Kristof Y Neven ◽  
Nelly D Saenen ◽  
Letitzia Tarantini ◽  
Bram G Janssen ◽  
Wouter Lefebvre ◽  
...  
Keyword(s):  
Air Pollution ◽  
Dna Repair ◽  
Cohort Study ◽  
Prenatal Exposure ◽  
Promoter Methylation ◽  
Dna Repair Genes ◽  
Particulate Air Pollution ◽  
Methylation Of Dna ◽  
Age Cohort ◽  
Repair Genes

scholarly journals Gene Expression, DNA Methylation and Prognostic Significance of DNA Repair Genes in Human Bladder Cancer

2017 ◽  
Vol 42 (6) ◽  
pp. 2404-2417 ◽  
Author(s):  
Anita Wojtczyk-Miaskowska ◽  
Malgorzata Presler ◽  
Jerzy Michajlowski ◽  
Marcin Matuszewski ◽  
Beata Schlichtholz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Bladder Cancer ◽  
Dna Repair ◽  
Mrna Expression ◽  
Promoter Methylation ◽  
Methylation Status ◽  
Prognostic Significance ◽  
Muscle Invasive ◽  
Repair Genes

Background/Aims: This study investigated the gene expression and DNA methylation of selected DNA repair genes (MBD4, TDG, MLH1, MLH3) and DNMT1 in human bladder cancer in the context of pathophysiological and prognostic significance. Methods: To determine the relationship between the gene expression pattern, global methylation and promoter methylation status, we performed real-time PCR to quantify the mRNA of selected genes in 50 samples of bladder cancer and adjacent non-cancerous tissue. The methylation status was analyzed by methylation-specific polymerase chain reaction (MSP) or digestion of genomic DNA with a methylation-sensitive restriction enzyme and PCR with gene-specific primers (MSRE-PCR). The global DNA methylation level was measured using the antibody-based 5-mC detection method. Results: The relative levels of mRNA for MBD4, MLH3, and MLH1 were decreased in 28% (14/50), 34% (17/50) and 36% (18/50) of tumor samples, respectively. The MBD4 mRNA expression was decreased in 46% of non-muscle invasive tumors (Ta/T1) compared with 11% found in muscle invasive tumors (T2-T4) (P<0.003). Analysis of mRNA expression for TDG did not show any significant differences between Ta/T1 and T2-T4 tumors. The frequency of increased DNMT1 mRNA expression was higher in T2-T4 (52%) comparing to Ta/T1 (16%). The overall methylation rates in tumor tissue were 18% for MBD4, 25% for MLH1 and there was no evidence of MLH3 promoter methylation. High grade tumors had significantly lower levels of global DNA methylation (P=0.04). There was a significant association between shorter survival and increased expression of DNMT1 mRNA (P=0.002), decreased expression of MLH1 mRNA (P=0.032) and the presence of MLH1 promoter methylation (P=0.006). Conclusion: This study highlights the importance of DNA repair pathways and provides the first evidence of the role of MBD4 and MLH3 in bladder cancer. In addition, our findings suggest that DNMT1 mRNA and MLH1 mRNA expression, as well as the status of MLH1 promoter methylation, are attractive prognostic markers in this pathology.

Sign in / Sign up

Export Citation Format

Share Document