scholarly journals Assessment of DNA damage in underground coal miners using the cytokinesis-block micronucleus assay in peripheral blood lymphocytes

Mutagenesis ◽  
2016 ◽  
Vol 31 (6) ◽  
pp. 669-675 ◽  
Author(s):  
Maxim Yu. Sinitsky ◽  
Varvara I. Minina ◽  
Nikolay I. Gafarov ◽  
Maxim A. Asanov ◽  
Aleksey V. Larionov ◽  
...  
Keyword(s):  
Dna Damage ◽  
Peripheral Blood ◽  
Micronucleus Assay ◽  
Peripheral Blood Lymphocytes ◽  
Coal Miners ◽  
Blood Lymphocytes

Assessment of Peripheral Blood Lymphocytes in Parents of Autistic Children by Cytokinesis Block Micronucleus Assay

2021 ◽  
Author(s):  
Parisa moeinian ◽  
Rasoul Alizadeh ◽  
Mitra Hakim Shooshtari ◽  
Hossein Mozdarani ◽  
Fatemeh Yousefi Laksari ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Micronucleus Assay ◽  
Peripheral Blood Lymphocytes ◽  
Autistic Children ◽  
Blood Lymphocytes

scholarly journals Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Francisco J. Pellegrino ◽  
Analía Risso ◽  
Yanina Corrada ◽  
Rocío C. Gambaro ◽  
Analía I. Seoane
Keyword(s):  
Dna Damage ◽  
Fish Oil ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Dietary Fish ◽  
Healthy Dogs ◽  
Fish Oil Supplementation

scholarly journals Analysis of 177Lu-DOTA-Octreotate Therapy-Induced DNA Damage in Peripheral Blood Lymphocytes of Patients with Neuroendocrine Tumors

2015 ◽  
Vol 56 (4) ◽  
pp. 505-511 ◽  
Author(s):  
D. Denoyer ◽  
P. Lobachevsky ◽  
P. Jackson ◽  
M. Thompson ◽  
O. A. Martin ◽  
...  
Keyword(s):  
Dna Damage ◽  
Neuroendocrine Tumors ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes

scholarly journals SMOKING GENOTOXICITY IN PERIPHERAL BLOOD LYMPHOCYTES USING THE COMET ASSAY

2013 ◽  
Vol 03 (03) ◽  
pp. 038-041
Author(s):  
Shobha S. Shetty ◽  
Hrishikesh Nachane
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
The Body ◽  
P Value ◽  
Tail Moment ◽  
Blood Lymphocytes ◽  
Significant Difference ◽  
Almost All

Abstract Background: Smoking has been shown to have a positive effect on DNA damage in almost all the cells of the body. Quantitative analysis of this damage will help in assessing the etiopathogenesis of various nicotine induced damage to the body. Comet assay has been an emerging tool in this regard and hence was applied by us to estimate the severity of DNA damage in smokers. Aims & Objectives: To evaluate the DNA genotoxicity in peripheral blood lymphocytes in smokers and their comparison with non smokers & assess the quantitative damage. Materials and methods: 30 smokers & 20 non smokers were recruited & their peripheral blood was taken for the comet assay to look for Olive moment & Tail moment to quantitatively assess the DNA damage due to cigarette smoking. Results: In our study there was no significant difference in the analysis of DNA damage (with regard to tail moment & olive moment) in smokers versus non smokers (P value: more than 0.05). Conclusions: Though smoking is known to cause DNA damage, we did not find significant differences between the two groups probably due to other multifactorial etiologies for genotoxicity.

scholarly journals CYTOTOXIC EFFECTS OF ARIPIPRAZOLE ON MKN45 AND NIH3T3 CELL LINES AND GENOTOXIC EFFECTS ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES

2019 ◽  
Vol 56 (2) ◽  
pp. 155-159 ◽  
Author(s):  
Mohammad SHOKRZADEH ◽  
Abbas MOHAMMADPOUR ◽  
Mona MODANLOO ◽  
Melika HASSANI ◽  
Nasrin Ghassemi BARGHI ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Line ◽  
Cell Lines ◽  
Peripheral Blood ◽  
Normal Cell ◽  
Micronucleus Assay ◽  
Peripheral Blood Lymphocytes ◽  
Nih3t3 Cell ◽  
Dopamine Antagonists ◽  
Blood Lymphocytes

ABSTRACT BACKGROUND: Gastric cancer is known as the fourth most common cancer. Current treatments for cancer have damaged the sensitive tissues of the healthy body, and in many cases, cancer will be recurrent. Therefore, need for treatments that are more effective is well felt. Researchers have recently shifted their attention towards antipsychotic dopamine antagonists to treat cancer. The anticancer activities of aripiprazole remain unknown. OBJECTIVE: This study aimed to evaluate the efficacy and safety of aripiprazole on gastric cancer and normal cell lines. METHODS: In this regard, the cytotoxicity and genotoxicity of aripiprazole were investigated in MKN45 and NIH3T3 cell lines by methyl tetrazolium assay and on peripheral blood lymphocytes by micronucleus assay. For this purpose, cells were cultured in 96 wells plate. Stock solutions of aripiprazole and cisplatin were prepared. After cell incubation with different concentrations of aripiprazole (1, 10, 25, 50, 100 and 200 μL), methyl tetrazolium solution was added. For micronucleus assay fresh blood was added to RPMI culture medium 1640 supplemented, and different concentrations of aripiprazole (50, 100 and 200 μL) were added. RESULTS: The finding of present study showed that the IC50 of aripiprazole in the cancer cell line (21.36 μg/mL) was lower than that in the normal cell line (54.17 μg/mL). Moreover, the micronucleus assay showed that the frequency of micronuclei of aripiprazole at concentrations below 200 μM was much less than cisplatin. CONCLUSION: Aripiprazole can be a good cytotoxic compound and good candidate for further studies of cancer therapy.

Biomonitoring of DNA damage in peripheral blood lymphocytes of subjects with dental restorative fillings

2008 ◽  
Vol 650 (2) ◽  
pp. 115-122 ◽  
Author(s):  
Angela Di Pietro ◽  
Giuseppa Visalli ◽  
Sebastiano La Maestra ◽  
Rosanna Micale ◽  
Barbara Baluce ◽  
...  
Keyword(s):  
Dna Damage ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Dental Restorative
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