scholarly journals Insensitivity of the in vitro cytokinesis-block micronucleus assay with human lymphocytes for the detection of DNA damage present at the start of the cell culture

Mutagenesis ◽  
2012 ◽  
Vol 27 (6) ◽  
pp. 743-747 ◽  
Author(s):  
G. Speit ◽  
R. Linsenmeyer ◽  
P. Schutz ◽  
S. Kuehner
Keyword(s):  
Cell Culture ◽  
Dna Damage ◽  
Human Lymphocytes ◽  
Micronucleus Assay

scholarly journals A comparison of the in vitro genotoxicity of anticancer drugs idarubicin and mitoxantrone.

2002 ◽  
Vol 49 (1) ◽  
pp. 145-155 ◽  
Author(s):  
Janusz Błasiak ◽  
Ewa Gloc ◽  
Mariusz Warszawski
Keyword(s):  
Dna Damage ◽  
Human Lymphocytes ◽  
Dna Glycosylase ◽  
Oxygen Species ◽  
Anthracycline Antibiotic ◽  
Strand Breaks ◽  
Endonuclease Iii ◽  
Normal Human ◽  
In Vitro Genotoxicity

Idarubicin is an anthracycline antibiotic used in cancer therapy. Mitoxantrone is an anthracycline analog with presumed better antineoplastic activity and lesser toxicity. Using the alkaline comet assaywe showed that the drugs at 0.01-10 microM induced DNA damage in normal human lymphocytes. The effect induced by idarubicin was more pronounced than by mitoxantrone (P < 0.001). The cells treated with mitoxantrone at 1 microM were able to repair damage to their DNA within a 30-min incubation, whereas the lymphocytes exposed to idarubicin needed 180 min. Since anthracyclines are known to produce free radicals, we checked whether reactive oxygen species might be involved in the observed DNA damage. Catalase, an enzyme inactivating hydrogen peroxide, decreased the extent of DNA damage induced by idarubicin, but did not affect the extent evoked by mitoxantrone. Lymphocytes exposed to the drugs and treated with endonuclease III or formamidopyrimidine-DNA glycosylase (Fpg), enzymes recognizing and nicking oxidized bases, displayed a higher level of DNA damage than the untreated ones. 3-Methyladenine-DNA glycosylase II (AlkA), an enzyme recognizing and nicking mainly methylated bases in DNA, increased the extent of DNA damage caused by idarubicin, but not that induced by mitoxantrone. Our results indicate that the induction of secondary malignancies should be taken into account as side effects of the two drugs. Direct strand breaks, oxidation and methylation of the DNA bases can underlie the DNA-damaging effect of idarubicin, whereas mitoxantrone can induce strand breaks and modification of the bases, including oxidation. The observed in normal lymphocytes much lesser genotoxicity of mitoxantrone compared to idarubicin should be taken into account in planning chemotherapeutic strategies.

Induction of oxidative DNA damage and enhancement of cell proliferation in human lymphocytes in vitro by butylated hydroxyanisole

1995 ◽  
Vol 16 (3) ◽  
pp. 507-512 ◽  
Author(s):  
P.A.E.L. Schilderman ◽  
E. Rhijnsburger ◽  
I. Zwingmann ◽  
J.C.S. Kleinjans
Keyword(s):  
Cell Proliferation ◽  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Human Lymphocytes ◽  
Butylated Hydroxyanisole

scholarly journals Micronucleus assay in human lymphocytes after exposure to alloxydim sodium herbicide in vitro

2014 ◽  
Vol 67 (6) ◽  
pp. 1059-1066 ◽  
Author(s):  
Dilek Akyıl ◽  
Arzu Özkara ◽  
S. Feyza Erdoğmuş ◽  
Yasin Eren ◽  
Muhsin Konuk ◽  
...  
Keyword(s):  
Human Lymphocytes ◽  
Micronucleus Assay

Resveratrol affects DNA damage induced by ionizing radiation in human lymphocytes in vitro

2016 ◽  
Vol 806 ◽  
pp. 40-46 ◽  
Author(s):  
Emiliano Basso ◽  
Giulia Regazzo ◽  
Mario Fiore ◽  
Valentina Palma ◽  
Gianandrea Traversi ◽  
...  
Keyword(s):  
Dna Damage ◽  
Ionizing Radiation ◽  
Human Lymphocytes

Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro

2015 ◽  
Vol 29 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Ninoslav Djelić ◽  
Milena Radaković ◽  
Biljana Spremo-Potparević ◽  
Lada Živković ◽  
Vladan Bajić ◽  
...  
Keyword(s):  
Dna Damage ◽  
Human Lymphocytes
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