scholarly journals In vitro evaluation of baseline and induced DNA damage in human sperm exposed to benzo[a]pyrene or its metabolite benzo[a]pyrene-7,8-diol-9,10-epoxide, using the comet assay

Mutagenesis ◽  
2010 ◽  
Vol 25 (4) ◽  
pp. 417-425 ◽  
Author(s):  
V. Sipinen ◽  
J. Laubenthal ◽  
A. Baumgartner ◽  
E. Cemeli ◽  
J. O. Linschooten ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Human Sperm ◽  
In Vitro Evaluation

DNA damage induced by coal dust, fly and bottom ash from coal combustion evaluated using the micronucleus test and comet assay in vitro

2017 ◽  
Vol 324 ◽  
pp. 781-788 ◽  
Author(s):  
Cristina Araujo Matzenbacher ◽  
Ana Letícia Hilario Garcia ◽  
Marcela Silva dos Santos ◽  
Caroline Cardoso Nicolau ◽  
Suziane Premoli ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Coal Combustion ◽  
Micronucleus Test ◽  
Coal Dust ◽  
Bottom Ash

scholarly journals Corrigendum to: Standardisation of the in vitro comet assay: influence of lysis time and lysis solution composition on the detection of DNA damage induced by X-rays

Mutagenesis ◽  
2019 ◽  
Vol 34 (5-6) ◽  
pp. 431-431
Author(s):  
José M Enciso ◽  
Kristine B Gutzkow ◽  
Gunnar Brunborg ◽  
Ann-Karin Olsen ◽  
Adela López de Cerain ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
X Rays ◽  
Solution Composition ◽  
Lysis Time

Evaluating the combinative effects on human lymphocyte DNA damage induced by Ultraviolet ray C plus 1.8GHz microwaves using comet assay in vitro

Toxicology ◽  
2007 ◽  
Vol 232 (3) ◽  
pp. 311-316 ◽  
Author(s):  
Wang Baohong ◽  
Jin Lifen ◽  
Li Lanjuan ◽  
Lou Jianlin ◽  
Lu Deqiang ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Human Lymphocyte ◽  
Ultraviolet Ray

scholarly journals Comet assay measures of DNA damage are predictive of bladder cancer cell treatment sensitivity in vitro and outcome in vivo

2013 ◽  
Vol 134 (5) ◽  
pp. 1102-1111 ◽  
Author(s):  
Karen J. Bowman ◽  
Manar M. Al‐Moneef ◽  
Benedict T. Sherwood ◽  
Alexandra J. Colquhoun ◽  
Jonathan C. Goddard ◽  
...  
Keyword(s):  
Dna Damage ◽  
Bladder Cancer ◽  
Comet Assay ◽  
Cancer Cell ◽  
Bladder Cancer Cell ◽  
Treatment Sensitivity

Effect of oxidative stress on development and DNA damage in in-vitro cultured bovine embryos by comet assay

2000 ◽  
Vol 54 (1) ◽  
pp. 137-145 ◽  
Author(s):  
M. Takahashi ◽  
K. Keicho ◽  
H. Takahashi ◽  
H. Ogawa ◽  
R.M. Schulte ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Comet Assay ◽  
Bovine Embryos

scholarly journals Genotoxic Effect of Arsenate and Arsenite in Human HaCat Cells in Culture Using Comet Assay

2020 ◽  
pp. 36-44
Author(s):  
Ghazalla Benhusein ◽  
Elaine Mutch ◽  
Faith M. Williams
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Statistical Significance ◽  
Human Keratinocytes ◽  
Hacat Cells ◽  
Environmental Chemical ◽  
Viable Cells ◽  
Buthionine Sulphoximine ◽  
Ros Formation

Arsenic is an environmental chemical of toxicological concern today since it is a human genotoxin and chronic exposure is associated with development of cancers, including skin. Inorganic arsenate is metabolically reduced to arsenite by glutathione (GSH) prior to methylation. The aim of this study was to determine the relative toxic effects of arsenate and arsenite in HaCat cells (immortalized human keratinocytes) in vitro by measuring cytotoxicity, DNA damage, depletion of glutathione and apoptotic and necrotic events. HaCat cells were treated with arsenate and arsenite (10 μM) for DNA damage detection using Comet assay and cytotoxicity (10, 60 and 100 μM) all measured at 24 hr. In some experiment arsenate or arsenite (10 μM) was added at the same time as BSO 10 μM for 24 hr, and GSH levels were measured by HPLC with fluorescence detection. Flow cytometry was used to investigate apoptotic and necrotic events following arsenate and arsenite (10 μM) treatment for 24 hr. Arsenate and arsenite at 60 and 100 μM, but not 10 μM, reduced the number of adherent viable cells with time. Therefore, DNA damage could only be measured at 10 μM as at higher concentrations the cells did not produce classical Comets but showed fragmentation. DNA damage was significantly (p < 0.001) increased in cells treated for 24 hr with 10 μM arsenate and arsenite compared to control. GSH levels were significantly increased in HaCat cells treated with10 μM arsenate and arsenite (p < 0.05, p < 0.001, respectively) compared to control. Cells treated with buthionine sulphoximine (BSO) at the same time as arsenate had increased GSH levels (p < 0.001), but arsenite and BSO did not increase cellular GSH. Arsenate and arsenite increased apoptosis, and arsenate increased necrosis, although none of the values reached statistical significance. Arsenite was more cytotoxic than arsenate. Arsenate and arsenite are known to produce oxidative stress involving ROS formation and depletion of glutathione. The increase in GSH levels at low doses of arsenate and arsenite, and by arsenate even in the presence of BSO.

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