Comparative studies of in vitro transformation by ethylene oxide and gamma-radiation of C3H/10T1/2 cells

Mutagenesis ◽  
1989 ◽  
Vol 4 (1) ◽  
pp. 58-61 ◽  
Author(s):  
Ada Kolman ◽  
Maria Näslund ◽  
Siv Osterman-Golkar ◽  
Gian-Paolo Scalia-Tomba ◽  
Anthony Meyer
Keyword(s):  
Ethylene Oxide ◽  
Gamma Radiation ◽  
Comparative Studies ◽  
In Vitro Transformation

scholarly journals In Vitro Evaluation of Acellular Collagen Matrices Derived from Porcine Pericardium: Influence of the Sterilization Method on Its Biological Properties

Materials ◽  
2021 ◽  
Vol 14 (21) ◽  
pp. 6255
Author(s):  
Rone Aparecido De Grandis ◽  
Larissa Natiele Miotto ◽  
Luis Eduardo Genaro ◽  
Larissa Migliatti Polli ◽  
Ana Maria de Guzzi Plepis ◽  
...  
Keyword(s):  
Ethylene Oxide ◽  
Gamma Radiation ◽  
In Vitro Assays ◽  
Membrane Thickness ◽  
Scanning Calorimetry ◽  
Collagen Matrices ◽  
Sterilization Method ◽  
Significant Difference ◽  
Porcine Pericardium

The aim of this study were characterize acellular collagen matrices derived from porcine pericardium (PP) and to evaluate their properties after sterilization by ethylene oxide and gamma ray. PP matrices were subjected to alkaline hydrolysis (AH), and samples were characterized for biological stability, membrane thickness measurements, differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). Subsequently, the matrices were frozen, lyophilized and sterilized by ethylene oxide or gamma radiation. For in vitro assays, CHO-K1 cell culture was used and evaluated for cytotoxicity, clonogenic survival assay, genotoxicity and mutagenicity. Analysis of variance (ANOVA) was used, followed by Dunnett’s post-test, with a significance level of 5%. After AH, there was no significant change in matrix thickness. The relative biodegradability of the material after implantation was observed. Morphology and dimensions had small changes after AH. As for cell viability, none of the tested matrices showed a statistically significant difference (p > 0.05; Dunnett) regardless of the sterilization method. Furthermore, it was found that PP matrices did not interfere with the proliferation capacity of CHO-K1 cells (p > 0.05; Dunnett). As for genotoxicity, when sterilized with ethylene oxide (NP, P12 and P24), it showed genotoxic potential, but it was not genotoxic when sterilized by gamma radiation. No mutagenic effects were observed in either group. PP-derived collagen matrices hydrolyzed at different times were not cytotoxic. It is concluded that the best method of sterilization is through gamma radiation, since no significant changes were observed in the properties of the PP matrices.

scholarly journals In vitro release of cytotoxic nucleoside analogs from lactide-caprolactone and lactide-glycolide copolymers.

2002 ◽  
Vol 49 (1) ◽  
pp. 205-210 ◽  
Author(s):  
Tomasz Kryczka ◽  
Maciej Bero ◽  
Janusz Kasperczyk ◽  
Piotr Dobrzyński ◽  
Barbara Marciniec ◽  
...  
Keyword(s):  
Ethylene Oxide ◽  
Gamma Radiation ◽  
Local Treatment ◽  
In Vitro Release ◽  
Nucleoside Analogs ◽  
Further Development ◽  
Kinetics Of ◽  
Vitro Release ◽  
Observation Period

The aims of our study were to assess the release of cytotoxic nucleoside analogs 5-fluorouracil and 2-chloro-2'-deoxyadenosine from different lactide-glycolide or lactide-caprolactone biodegradable copolymers and the effects of sterilization on this release. The polymers were sterilized either with ethylene oxide at 37 degrees C, or with gamma radiation (15 kGy, 20 kGy, or 25 kGy). The kinetics of nucleoside release from the copolymers were measured over 50 days. Four copolymers exhibited relatively constant release of nucleosides in micromolar concentrations during the entire observation period. Sterilization with either ethylene oxide or gamma radiation only slightly influenced nucleoside release. Further development of these copolymers as an intracerebral nucleoside delivery system for local treatment of brain tumors is indicated.

COMPARATIVE STUDIES OF THE POTENTIATION OF CORTICOTROPHIN ACTIVITY BY SEVERAL INACTIVE DERIVATIVES

1963 ◽  
Vol 42 (2) ◽  
pp. 209-213 ◽  
Author(s):  
Arthur I. Cohen ◽  
Edward H. Frieden
Keyword(s):  
Biological Activity ◽  
Comparative Studies ◽  
Free Amino ◽  
Hormonal Activity ◽  
Amino Groups

ABSTRACT A number of corticotrophin analogues have been prepared, some of which potentiate the biological activity of the untreated hormone in vitro. The free amino groups of corticotrophin appear to be essential not only for hormonal activity, but also for the interaction of the analogues with the tissue corticotrophin inactivating system which is assumed to account for the potentiating effect.

scholarly journals Comparative studies on Salmonella typhi grown in vivo and in vitro III. The immunizing potencies of acetone-killed vaccines prepared from in vivo and in vitro grown bacteria and the immunizing potency of substances isolated from infected organs

1963 ◽  
Vol 61 (3) ◽  
pp. 353-363 ◽  
Author(s):  
A. L. Olitzki ◽  
Dina Godinger
Keyword(s):  
Comparative Studies ◽  
Guinea Pigs ◽  
Peritoneal Fluid ◽  
Parent Strain ◽  
Salmonella Typhi ◽  
Challenge Strain

1. Salmonella typhi, strain Ty2, grown in vivo and employed as acetone-dried vaccine possessed a higher immunizing potency than the descendants of the same parent strain grown in vitro and employed as vaccine.2. When 2 × 108in vitro-grown bacteria were employed as challenge, the immunizing effects of both types of vaccine were more marked than after administration of 2 × 108in vivo-grown bacteria as challenge.3. The higher potency of the in vivo-grown vaccine was apparent in all experiments, whether the challenge strain was grown in vivo or in vitro.4. Immunogenic substances were isolated from infected organs of mice and guinea-pigs, and an immunogenic substance from the peritoneal fluid of the infected guinea-pigs was concentrated by precipitation with ethanol.

scholarly journals Phosphorylation of bovine leukemia virus Tax protein is required for in vitro transformation but not for transactivation

Oncogene ◽  
1998 ◽  
Vol 16 (17) ◽  
pp. 2165-2176 ◽  
Author(s):  
Luc Willems ◽  
Cathy Grimonpont ◽  
Pierre Kerkhofs ◽  
Carine Capiau ◽  
Dirk Gheysen ◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
In Vitro Transformation ◽  
Tax Protein

scholarly journals Comparative studies of cellular viability levels on 2D and 3D in vitro culture matrices

2017 ◽  
Vol 70 (1) ◽  
pp. 261-273 ◽  
Author(s):  
M. Gargotti ◽  
U. Lopez-Gonzalez ◽  
H. J. Byrne ◽  
A. Casey
Keyword(s):  
In Vitro Culture ◽  
Comparative Studies ◽  
Cellular Viability ◽  
2D And 3D

Human colon cells: culture and in vitro transformation

Science ◽  
1984 ◽  
Vol 224 (4656) ◽  
pp. 1445-1447 ◽  
Author(s):  
M. Moyer ◽  
J. Aust
Keyword(s):  
Human Colon ◽  
Colon Cells ◽  
In Vitro Transformation
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