Very low doses of X-rays can cause human lymphocytes to become less susceptible to ionizing radiation

Mutagenesis ◽  
1987 ◽  
Vol 2 (2) ◽  
pp. 95-96 ◽  
Author(s):  
Jeffery D. Shadley ◽  
Sheldon Wolff
Keyword(s):  
Ionizing Radiation ◽  
Human Lymphocytes ◽  
Low Doses ◽  
X Rays

Chromosomal Aberrations in Human Lymphocytes Inducedin Vitroby Very Low Doses of X-rays

1992 ◽  
Vol 61 (3) ◽  
pp. 335-343 ◽  
Author(s):  
D.C. Lloyd ◽  
A.A. Edwards ◽  
A. Leonard ◽  
G.L. Deknudt ◽  
L. Verschaeve ◽  
...  
Keyword(s):  
Chromosomal Aberrations ◽  
Human Lymphocytes ◽  
Low Doses ◽  
X Rays

scholarly journals Development of thyroid pathology in medical workers with chronic exposure to low doses of ionizing radiation

2021 ◽  
Vol 20 (5) ◽  
pp. 90-97
Author(s):  
L. D. Gordienko ◽  
T. P. Kiseleva ◽  
I. I. Gordienko ◽  
N. A. Tsap
Keyword(s):  
Experimental Data ◽  
Ionizing Radiation ◽  
Thyroid Hormones ◽  
Chronic Exposure ◽  
Low Dose ◽  
Thyroid Tissue ◽  
Medical Personnel ◽  
Low Doses ◽  
X Rays ◽  
Intercellular Contacts

Introduction. The literature review deals with the effects of ionizing radiation and radiation on the body of medical personnel.Materials and methods. 50 actual sources were analyzed. The relevance of the study of the constant, long-term influence of low doses of radiation is shown, in view of the increasing use of sources of ionizing radiation in various fields of scientific and practical human activity, including medicine.Results. Thanks to the analysis of numerous literature data, it was revealed that operating doctors, who are faced in their work with sources of ionizing radiation, often do not have a sufficient level of protection of the thyroid tissue from radiation. It was revealed that the overwhelming majority of studies highlighting thyroid pathology in medical personnel working with X-rays are aimed at identifying the risks of thyroid cancer and does not take into account the pathology of non-tumor genesis. Analyzed the data of scientific publications, allowing to draw a conclusion about the effects of chronic exposure to ionizing radiation, namely X-rays, in low doses on the thyroid tissue from a morphological and functional point of view.Discussion. It was established on the basis of the analysis of experimental data that under the influence of low-dose X-ray radiation, the intercellular contacts of thyrocytes are disconnected, which can lead to a decrease in the synthesis of thyroid hormones and, as a consequence, to the development of hypothyroidism.Conclusion. Based on the analysis of the experimental data, it has been established that under the influence of low-dose X-rays there is a disconnection of intercellular contacts of thyrocytes, which may lead to a decrease in the synthesis of thyroid hormones and, consequently, to the development of hypothyroidism.

Human lymphocytes exposed to low doses of X-rays are less susceptible to radiation-induced mutagenesis

1991 ◽  
Vol 263 (4) ◽  
pp. 197-201 ◽  
Author(s):  
K.T. Kelsey ◽  
A. Memisoglu ◽  
D. Frenkel ◽  
H.L. Liber
Keyword(s):  
Human Lymphocytes ◽  
Low Doses ◽  
X Rays ◽  
Radiation Induced ◽  
Induced Mutagenesis

scholarly journals Biodosimetry of Low Dose Ionizing Radiation Using DNA Repair Foci in Human Lymphocytes

Genes ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 58 ◽  
Author(s):  
Lukáš Jakl ◽  
Eva Marková ◽  
Lucia Koláriková ◽  
Igor Belyaev
Keyword(s):  
Dna Repair ◽  
Ionizing Radiation ◽  
Low Dose ◽  
Time Window ◽  
Human Lymphocytes ◽  
High Dose ◽  
Low Doses ◽  
Significant Difference ◽  
Kinetics Of ◽  
Higher Doses

Purpose: Ionizing radiation induced foci (IRIF) known also as DNA repair foci represent most sensitive endpoint for assessing DNA double strand breaks (DSB). IRIF are usually visualized and enumerated with the aid of fluorescence microscopy using antibodies to γH2AX and 53BP1. This study analyzed effect of low dose ionizing radiation on residual IRIF in human lymphocytes to the aim of potential biodosimetry and possible extrapolation of high-dose γH2AX/53BP1 effects to low doses and compared kinetics of DSB and IRIF. We also analyzed whether DNaseI, which is used for reducing of clumps, affects the IRIF level. Materials and Methods: The cryopreserved human lymphocytes from umbilical cord blood (UCB) were thawed with/without DNaseI, γ-irradiated at doses of 0, 5, 10, and 50 cGy and γH2AX/53BP1 foci were analyzed 30 min, 2 h, and 22 h post-irradiation using appropriate antibodies. We also analyzed kinetics of DSB using PFGE. Results: No significant difference was observed between data obtained by γH2AX foci evaluation in cells that were irradiated by low doses and data obtained by extrapolation from higher doses. Residual 53BP1 foci induced by low doses significantly outreached the data extrapolated from irradiation by higher doses. 53BP1 foci induced by low dose-radiation remain longer at DSB loci than foci induced by higher doses. There was no significant effect of DNaseI on DNA repair foci. Conclusions: Primary γH2AX, 53BP1 foci and their co-localization represent valuable markers for biodosimetry of low doses, but their usefulness is limited by short time window. Residual γH2AX and 53BP1 foci are more useful markers for biodosimetry in vitro. Effects of low doses can be extrapolated from high dose using γH2AX residual foci while γH2AX/53BP1 foci are valuable markers for evaluation of initial DSB induced by ionizing radiation. Residual IRIF induced by low doses persist longer time than those induced by higher doses.

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