scholarly journals Extracellular ATP Promotes Stomatal Opening of Arabidopsis thaliana through Heterotrimeric G Protein α Subunit and Reactive Oxygen Species

2012 ◽  
Vol 5 (4) ◽  
pp. 852-864 ◽  
Author(s):  
Li-Hua Hao ◽  
Wei-Xia Wang ◽  
Chen Chen ◽  
Yu-Fang Wang ◽  
Ting Liu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Arabidopsis Thaliana ◽  
G Protein ◽  
Reactive Oxygen ◽  
Extracellular Atp ◽  
Stomatal Opening ◽  
Oxygen Species ◽  
Heterotrimeric G Protein ◽  
Α Subunit ◽  
G Protein Α Subunit

scholarly journals Mitogen-Activated Protein Kinase Phosphatase 1 (MKP1) Negatively Regulates the Production of Reactive Oxygen Species During Arabidopsis Immune Responses

2019 ◽  
Vol 32 (4) ◽  
pp. 464-478 ◽  
Author(s):  
Viviana Escudero ◽  
Miguel Ángel Torres ◽  
Magdalena Delgado ◽  
Sara Sopeña-Torres ◽  
Sanjay Swami ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Protein Kinase ◽  
Immune Responses ◽  
G Protein ◽  
Reactive Oxygen ◽  
Mitogen Activated Protein Kinase ◽  
Oxygen Species ◽  
Heterotrimeric G Protein ◽  
Genetic Ablation ◽  
Mitogen Activated Protein

Genetic ablation of the β subunit of the heterotrimeric G protein complex in agb1-2 confers defective activation of microbe-associated molecular pattern (MAMP)-triggered immunity, resulting in agb1-2 enhanced susceptibility to pathogens like the fungus Plectosphaerella cucumerina BMM. A mutant screen for suppressors of agb1-2 susceptibility (sgb) to P. cucumerina BMM identified sgb10, a new null allele (mkp1-2) of the mitogen-activated protein kinase phosphatase 1 (MKP1). The enhanced susceptibility of agb1-2 to the bacterium Pseudomonas syringae pv. tomato DC3000 and the oomycete Hyaloperonospora arabidopsidis is also abrogated by mkp1-2. MKP1 negatively balances production of reactive oxygen species (ROS) triggered by MAMPs, since ROS levels are enhanced in mkp1. The expression of RBOHD, encoding a NADPH oxidase–producing ROS, is upregulated in mkp1 upon MAMP treatment or pathogen infection. Moreover, MKP1 negatively regulates RBOHD activity, because ROS levels upon MAMP treatment are increased in mkp1 plants constitutively overexpressing RBOHD (35S::RBOHD mkp1). A significant reprograming of mkp1 metabolic profile occurs with more than 170 metabolites, including antimicrobial compounds, showing differential accumulation in comparison with wild-type plants. These results suggest that MKP1 functions downstream of the heterotrimeric G protein during MAMP-triggered immunity, directly regulating the activity of RBOHD and ROS production as well as other immune responses.

scholarly journals Rational design of a heterotrimeric G protein α subunit with artificial inhibitor sensitivity

2019 ◽  
Vol 294 (15) ◽  
pp. 5747-5758 ◽  
Author(s):  
Davide Malfacini ◽  
Julian Patt ◽  
Suvi Annala ◽  
Kasper Harpsøe ◽  
Funda Eryilmaz ◽  
...  
Keyword(s):  
G Protein ◽  
Rational Design ◽  
Heterotrimeric G Protein ◽  
Α Subunit ◽  
G Protein Α Subunit

The Receptor-Bound “Empty Pocket” State of the Heterotrimeric G-Protein α-Subunit Is Conformationally Dynamic†

Biochemistry ◽  
2006 ◽  
Vol 45 (43) ◽  
pp. 12986-12997 ◽  
Author(s):  
Najmoutin G. Abdulaev ◽  
Tony Ngo ◽  
Eva Ramon ◽  
Danielle M. Brabazon ◽  
John P. Marino ◽  
...  
Keyword(s):  
G Protein ◽  
Heterotrimeric G Protein ◽  
Α Subunit ◽  
G Protein Α Subunit

GNG11 (G-protein subunit γ 11) suppresses cell growth with induction of reactive oxygen species and abnormal nuclear morphology in human SUSM-1 cells

2017 ◽  
Vol 95 (4) ◽  
pp. 517-523 ◽  
Author(s):  
Yuki Takauji ◽  
Ikuru Kudo ◽  
Atsuki En ◽  
Ryo Matsuo ◽  
Mohammad Nazir Hossain ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
G Protein ◽  
Cell Lines ◽  
Hela Cells ◽  
Reactive Oxygen ◽  
Ros Generation ◽  
Nuclear Morphology ◽  
Oxygen Species ◽  
Protein Subunit ◽  
Regulatory Relationship

Enforced expression of GNG11, G-protein subunit γ 11, induces cellular senescence in normal human diploid fibroblasts. We here examined the effect of the expression of GNG11 on the growth of immortalized human cell lines, and found that it suppressed the growth of SUSM-1 cells, but not of HeLa cells. We then compared these two cell lines to understand the molecular basis for the action of GNG11. We found that expression of GNG11 induced the generation of reactive oxygen species (ROS) and abnormal nuclear morphology in SUSM-1 cells but not in HeLa cells. Increased ROS generation by GNG11 would likely be caused by the down-regulation of the antioxidant enzymes in SUSM-1 cells. We also found that SUSM-1 cells, even under normal culture conditions, showed higher levels of ROS and higher incidence of abnormal nuclear morphology than HeLa cells, and that abnormal nuclear morphology was relevant to the increased ROS generation in SUSM-1 cells. Thus, SUSM-1 and HeLa cells showed differences in the regulation of ROS and nuclear morphology, which might account for their different responses to the expression of GNG11. Thus, SUSM-1 cells may provide a unique system to study the regulatory relationship between ROS generation, nuclear morphology, and G-protein signaling.

Ectopic ferredoxin I protein promotes root hair growth through induction of reactive oxygen species in Arabidopsis thaliana

2011 ◽  
Vol 168 (5) ◽  
pp. 434-440 ◽  
Author(s):  
Lung-Jiun Shin ◽  
Hsiang-En Huang ◽  
Hsiang Chang ◽  
Yi-Hsien Lin ◽  
Teng-Yung Feng ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Arabidopsis Thaliana ◽  
Root Hair ◽  
Hair Growth ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Root Hair Growth ◽  
Ferredoxin I

scholarly journals Heterotrimeric G protein α subunit is involved in rice brassinosteroid response

Cell Research ◽  
2006 ◽  
Vol 16 (12) ◽  
pp. 916-922 ◽  
Author(s):  
Lei Wang ◽  
Yun-Yuan Xu ◽  
Qi-Bin Ma ◽  
Dan Li ◽  
Zhi-Hong Xu ◽  
...  
Keyword(s):  
G Protein ◽  
Heterotrimeric G Protein ◽  
Α Subunit ◽  
G Protein Α Subunit

Metal-specific and NADPH oxidase dependent changes in lipoxygenase and NADPH oxidase gene expression in Arabidopsis thaliana exposed to cadmium or excess copper

2010 ◽  
Vol 37 (6) ◽  
pp. 532 ◽  
Author(s):  
Tony Remans ◽  
Kelly Opdenakker ◽  
Karen Smeets ◽  
Dennis Mathijsen ◽  
Jaco Vangronsveld ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reactive Oxygen Species ◽  
Arabidopsis Thaliana ◽  
Nadph Oxidase ◽  
Transcriptional Profiling ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Adaptive Responses ◽  
Oxidase Gene ◽  
Opposing Effects

Reactive oxygen species produced by NADPH oxidase and oxylipins derived from lipoxygenase activity can signal various stress conditions and have been implicated when plants are exposed to heavy metals. Transcriptional profiling of the 10 NADPH oxidase and 6 lipoxygenase genes was performed after exposure of Arabidopsis thaliana wild-type and NADPH oxidase mutants to 5 µM CdSO4 or 2 µM CuSO4 for 24 h. Under these short exposures to environmentally realistic concentrations of Cd or Cu, plants modulate signalling networks that regulate the onset of adaptive responses. Metal-specific NADPH oxidase genes were upregulated by Cd but downregulated by Cu, and metal-specific lipoxygenase gene expression was observed only after Cu exposure. Genes that are responsive to both metals were upregulated and may be responsive to general oxidative stress. For all metal-responsive genes except RBOHD, distinct responses were observed between leaves and roots, which may be due to different stress intensities and signalling mechanisms. Mutation of NADPH oxidase genes had opposing effects on gene expression after Cd or Cu exposure. Upregulation of LOX1 and LOX6 in the roots after exposure to Cd depended on NADPH oxidase gene expression, whereas LOX3 and LOX6 expression was induced more strongly in NADPH oxidase mutants after Cu exposure. Furthermore, NADPH oxidases regulated their own expression level and that of other members of the gene family when exposed to Cd or Cu. The results suggest interplay between reactive oxygen species and oxylipin signalling under Cd or Cu stress, and are useful as a basis for genetic studies to unravel metal-specific signalling mechanisms.

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