scholarly journals TPC1 – SV Channels Gain Shape

2011 ◽  
Vol 4 (3) ◽  
pp. 428-441 ◽  
Author(s):  
Rainer Hedrich ◽  
Irene Marten
Keyword(s):  
Sv Channels

scholarly journals Effect of Trimethyltin Chloride on Slow Vacuolar (SV) Channels in Vacuoles from Red Beet (Beta vulgaris L.) Taproots

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0136346 ◽  
Author(s):  
Zenon Trela ◽  
Zbigniew Burdach ◽  
Agnieszka Siemieniuk ◽  
Stanisław Przestalski ◽  
Waldemar Karcz
Keyword(s):  
Beta Vulgaris ◽  
Red Beet ◽  
Sv Channels ◽  
Trimethyltin Chloride

Effect of trimethyllead chloride on slowly activating (SV) channels in red beet (Beta vulgaris L.) taproots

2012 ◽  
Vol 335 (12) ◽  
pp. 722-730 ◽  
Author(s):  
Zenon Trela ◽  
Zbigniew Burdach ◽  
Stanisław Przestalski ◽  
Waldemar Karcz
Keyword(s):  
Beta Vulgaris ◽  
Red Beet ◽  
Sv Channels

SV channels dominate the vacuolar Ca2+release during intracellular signaling

FEBS Letters ◽  
2009 ◽  
Vol 583 (5) ◽  
pp. 921-926 ◽  
Author(s):  
Igor Pottosin ◽  
Tim Wherrett ◽  
Sergey Shabala
Keyword(s):  
Intracellular Signaling ◽  
Sv Channels

scholarly journals A Comparison of the Effect of Lead (Pb) on the Slow Vacuolar (SV) and Fast Vacuolar (FV) Channels in Red Beet (Beta vulgaris L.) Taproot Vacuoles

2021 ◽  
Vol 22 (23) ◽  
pp. 12621
Author(s):  
Agnieszka Siemieniuk ◽  
Zbigniew Burdach ◽  
Waldemar Karcz
Keyword(s):  
Single Channel ◽  
Channel Conductance ◽  
Single Channel Conductance ◽  
Patch Clamp Technique ◽  
Open Probability ◽  
Outward Currents ◽  
Inward Currents ◽  
Sv Channels ◽  
The One ◽  
The Impact

Little is known about the effect of lead on the activity of the vacuolar K+ channels. Here, the patch-clamp technique was used to compare the impact of lead (PbCl2) on the slow-activating (SV) and fast-activating (FV) vacuolar channels. It was revealed that, under symmetrical 100-mM K+, the macroscopic currents of the SV channels exhibited a typical slow activation and a strong outward rectification of the steady-state currents, while the macroscopic currents of the FV channels displayed instantaneous currents, which, at the positive potentials, were about three-fold greater compared to the one at the negative potentials. When PbCl2 was added to the bath solution at a final concentration of 100 µM, it decreased the macroscopic outward currents of both channels but did not change the inward currents. The single-channel recordings demonstrated that cytosolic lead causes this macroscopic effect by a decrease of the single-channel conductance and decreases the channel open probability. We propose that cytosolic lead reduces the current flowing through the SV and FV channels, which causes a decrease of the K+ fluxes from the cytosol to the vacuole. This finding may, at least in part, explain the mechanism by which cytosolic Pb2+ reduces the growth of plant cells.

scholarly journals Computational Analyses of the AtTPC1 (Arabidopsis Two-Pore Channel 1) Permeation Pathway

2021 ◽  
Vol 22 (19) ◽  
pp. 10345
Author(s):  
Carlos Navarro-Retamal ◽  
Stephan Schott-Verdugo ◽  
Holger Gohlke ◽  
Ingo Dreyer
Keyword(s):  
Free Energy ◽  
Relative Permeability ◽  
In Silico ◽  
Md Simulations ◽  
Free Energy Calculations ◽  
Pore Channel ◽  
Luminal Side ◽  
Electrophysiological Studies ◽  
Sv Channels ◽  
Computational Analyses

Two Pore Channels (TPCs) are cation-selective voltage- and ligand-gated ion channels in membranes of intracellular organelles of eukaryotic cells. In plants, the TPC1 subtype forms the slowly activating vacuolar (SV) channel, the most dominant ion channel in the vacuolar membrane. Controversial reports about the permeability properties of plant SV channels fueled speculations about the physiological roles of this channel type. TPC1 is thought to have high Ca2+ permeability, a conclusion derived from relative permeability analyses using the Goldman–Hodgkin–Katz (GHK) equation. Here, we investigated in computational analyses the properties of the permeation pathway of TPC1 from Arabidopsis thaliana. Using the crystal structure of AtTPC1, protein modeling, molecular dynamics (MD) simulations, and free energy calculations, we identified a free energy minimum for Ca2+, but not for K+, at the luminal side next to the selectivity filter. Residues D269 and E637 coordinate in particular Ca2+ as demonstrated in in silico mutagenesis experiments. Such a Ca2+-specific coordination site in the pore explains contradicting data for the relative Ca2+/K+ permeability and strongly suggests that the Ca2+ permeability of SV channels is largely overestimated from relative permeability analyses. This conclusion was further supported by in silico electrophysiological studies showing a remarkable permeation of K+ but not Ca2+ through the open channel.

scholarly journals Long range correlations of the ion current in SV channels. Met3PbCl influence study

PLoS ONE ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. e0229433 ◽  
Author(s):  
Janusz Miśkiewicz ◽  
Zenon Trela ◽  
Zbigniew Burdach ◽  
Waldemar Karcz ◽  
Wanda Balińska-Miśkiewicz
Keyword(s):  
Long Range ◽  
Ion Current ◽  
Long Range Correlations ◽  
Sv Channels

scholarly journals Different properties of SV channels in root vacuoles from near isogenic Al-tolerant and Al-sensitive wheat cultivars

FEBS Letters ◽  
2005 ◽  
Vol 579 (30) ◽  
pp. 6890-6894 ◽  
Author(s):  
Tim Wherrett ◽  
Sergey Shabala ◽  
Igor Pottosin
Keyword(s):  
Wheat Cultivars ◽  
Sv Channels

scholarly journals Role of auxin (IAA) in the regulation of slow vacuolar (SV) channels and the volume of red beet taproot vacuoles

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Zbigniew Burdach ◽  
Agnieszka Siemieniuk ◽  
Zenon Trela ◽  
Renata Kurtyka ◽  
Waldemar Karcz
Keyword(s):  
Red Beet ◽  
Sv Channels
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