scholarly journals Fluorescence Intensity Decay Shape Analysis Microscopy (FIDSAM) for Quantitative and Sensitive Live-Cell Imaging: A Novel Technique for Fluorescence Microscopy of Endogenously Expressed Fusion-Proteins

2010 ◽  
Vol 3 (3) ◽  
pp. 555-562 ◽  
Author(s):  
Frank Schleifenbaum ◽  
Kirstin Elgass ◽  
Marcus Sackrow ◽  
Katharina Caesar ◽  
Kenneth Berendzen ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Shape Analysis ◽  
Fluorescence Intensity ◽  
Fusion Proteins ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Novel Technique ◽  
Fluorescence Intensity Decay ◽  
Intensity Decay

scholarly journals Fluorescence intensity decay shape analysis microscopy (FIDSAM) for quantitative and sensitive live-cell imaging

2010 ◽  
Author(s):  
Sébastien Peter ◽  
Kirstin Elgass ◽  
Marcus Sackrow ◽  
Katharina Caesar ◽  
Anne-Kathrin Born ◽  
...  
Keyword(s):  
Shape Analysis ◽  
Fluorescence Intensity ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Fluorescence Intensity Decay ◽  
Intensity Decay

scholarly journals Engineered HaloTag variants for fluorescence lifetime multiplexing

2021 ◽  
Author(s):  
Michelle S. Frei ◽  
Miroslaw Tarnawski ◽  
M. Julia Roberti ◽  
Birgit Koch ◽  
Julien Hiblot ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescence Lifetime ◽  
Fusion Proteins ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Cellular Targets ◽  
Label Fusion ◽  
Spectral Channel ◽  
Protein Tags

AbstractSelf-labeling protein tags such as HaloTag are powerful tools that can label fusion proteins with synthetic fluorophores for use in fluorescence microscopy. Here we introduce HaloTag variants with either increased or decreased brightness and fluorescence lifetime compared with HaloTag7 when labeled with rhodamines. Combining these HaloTag variants enabled live-cell fluorescence lifetime multiplexing of three cellular targets in one spectral channel using a single fluorophore and the generation of a fluorescence lifetime-based biosensor. Additionally, the brightest HaloTag variant showed up to 40% higher brightness in live-cell imaging applications.

scholarly journals Full-field supercritical angle fluorescence microscopy for live cell imaging

2011 ◽  
Vol 36 (16) ◽  
pp. 3051 ◽  
Author(s):  
Thomas Barroca ◽  
Karla Balaa ◽  
Julie Delahaye ◽  
Sandrine Lévêque-Fort ◽  
Emmanuel Fort
Keyword(s):  
Fluorescence Microscopy ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Full Field

scholarly journals Live cell imaging of macrophage/bacterium interaction demonstrates cell lysis induced by Corynebacterium diphtheriae and Corynebacterium ulcerans

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Dulanthi Weerasekera ◽  
Jonas Hahn ◽  
Martin Herrmann ◽  
Andreas Burkovski
Keyword(s):  
Fluorescence Microscopy ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Time Lapse ◽  
Corynebacterium Diphtheriae ◽  
Live Cell ◽  
Human Macrophage ◽  
Data Description ◽  
Corynebacterium Ulcerans ◽  
Microscopy Data

Abstract Objectives In frame of a study to characterize the interaction of human macrophage-like cells with pathogenic corynebacteria, Corynebacterium diphtheriae and Corynebacterium ulcerans, live cell imaging experiments were carried out and time lapse fluorescence microscopy videos were generated, which are presented here. Data description The time lapse fluorescence microscopy data revealed new insights in the interaction of corynebacteria with human macrophage-like THP-1 cells. In contrast to uninfected cells and infections with non-pathogenic C. glutamicum used as a control, pathogenic C. diphtheriae and C. ulcerans showed highly detrimental effects towards human cells and induction of cell death of macrophages.

Live cell imaging with Surface Plasmon-Mediated Fluorescence Microscopy

2009 ◽  
Author(s):  
Karla Balaa ◽  
Viviane Devauges ◽  
Yannick Goulam ◽  
Vincent Studer ◽  
Sandrine Lévêque-Fort ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Surface Plasmon ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell
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