scholarly journals Identification of stable endogenous reference genes for real-time PCR in the human fetal gonad using an external standard technique

2011 ◽  
Vol 17 (10) ◽  
pp. 620-625 ◽  
Author(s):  
Peter J. O'Shaughnessy ◽  
Ana Monteiro ◽  
Paul A. Fowler
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Standard Technique ◽  
External Standard ◽  
Endogenous Reference ◽  
Endogenous Reference Genes

scholarly journals Human Circulating miRNAs Real-time qRT-PCR-based Analysis: An Overview of Endogenous Reference Genes Used for Data Normalization

2019 ◽  
Vol 20 (18) ◽  
pp. 4353 ◽  
Author(s):  
Simone Donati ◽  
Simone Ciuffi ◽  
Maria L. Brandi
Keyword(s):  
Real Time ◽  
Reference Genes ◽  
Biological Fluids ◽  
Quantitative Polymerase Chain Reaction ◽  
Expression Patterns ◽  
Transcriptional Level ◽  
Circulating Mirnas ◽  
Endogenous Reference ◽  
Mirnas Expression ◽  
Endogenous Reference Genes

miRNAs are small non-coding RNAs of about 18–25 nucleotides that negatively regulate gene expression at the post-transcriptional level. It was reported that a deregulation of their expression patterns correlates to the onset and progression of various diseases. Recently, these molecules have been identified in a great plethora of biological fluids, and have also been proposed as potential diagnostic and prognostic biomarkers. Actually, real time quantitative polymerase chain reaction is the most widely used approach for circulating miRNAs (c-miRNAs) expression profiling. Nevertheless, the debate on the choice of the most suitable endogenous reference genes for c-miRNAs expression levels normalization is still open. In this regard, numerous research groups are focusing their efforts upon identifying specific, highly stable, endogenous c-mRNAs. The aim of this review is to provide an overview on the reference genes currently used in the study of various pathologies, offering to researchers the opportunity to select the appropriate molecules for c-miRNA levels normalization, when their choosing is based upon literature data.

Evaluation of endogenous reference genes for analysis of gene expression with real-time RT-PCR during planarian regeneration

2010 ◽  
Vol 38 (7) ◽  
pp. 4423-4428 ◽  
Author(s):  
Yan-qing Yuwen ◽  
Zi-mei Dong ◽  
Qing-hua Wang ◽  
Xiao-juan Sun ◽  
Chang-ying Shi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Reference Genes ◽  
Rt Pcr ◽  
Planarian Regeneration ◽  
Endogenous Reference ◽  
Endogenous Reference Genes
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