scholarly journals New horizons for in vitro spermatogenesis? An update on novel three-dimensional culture systems as tools for meiotic and post-meiotic differentiation of testicular germ cells

2009 ◽  
Vol 15 (9) ◽  
pp. 521-529 ◽  
Author(s):  
J.-B. Stukenborg ◽  
S. Schlatt ◽  
M. Simoni ◽  
C.-H. Yeung ◽  
M. A. Elhija ◽  
...  
Keyword(s):  
Germ Cells ◽  
Three Dimensional ◽  
New Horizons ◽  
Culture Systems ◽  
In Vitro Spermatogenesis ◽  
Three Dimensional Culture

scholarly journals Spermatogonial Stem Cells for In Vitro Spermatogenesis and In Vivo Restoration of Fertility

Cells ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 745 ◽  
Author(s):  
Fahar Ibtisham ◽  
Ali Honaramooz
Keyword(s):  
Stem Cells ◽  
Germ Cells ◽  
Recent Progress ◽  
Male Germ Cells ◽  
Culture Systems ◽  
In Vitro Spermatogenesis ◽  
Haploid Male ◽  
Restoration Of Fertility

Spermatogonial stem cells (SSCs) are the only adult stem cells capable of passing genes onto the next generation. SSCs also have the potential to provide important knowledge about stem cells in general and to offer critical in vitro and in vivo applications in assisted reproductive technologies. After century-long research, proof-of-principle culture systems have been introduced to support the in vitro differentiation of SSCs from rodent models into haploid male germ cells. Despite recent progress in organotypic testicular tissue culture and two-dimensional or three-dimensional cell culture systems, to achieve complete in vitro spermatogenesis (IVS) using non-rodent species remains challenging. Successful in vitro production of human haploid male germ cells will foster hopes of preserving the fertility potential of prepubertal cancer patients who frequently face infertility due to the gonadotoxic side-effects of cancer treatment. Moreover, the development of optimal systems for IVS would allow designing experiments that are otherwise difficult or impossible to be performed directly in vivo, such as genetic manipulation of germ cells or correction of genetic disorders. This review outlines the recent progress in the use of SSCs for IVS and potential in vivo applications for the restoration of fertility.

scholarly journals A Review on Application of Three Dimensional Culture and Testicular Scaffolds to Induction of in-Vitro Spermatogenesis

2020 ◽  
Author(s):  
Nasrin Majidi Gharenaz ◽  
Mansoureh Movahedin ◽  
Samiyeh Majidi ◽  
Zohreh Mazaheri
Keyword(s):  
Stem Cells ◽  
Three Dimensional ◽  
3D Culture ◽  
Spermatogonial Stem Cells ◽  
Ethical Challenges ◽  
Agar Culture ◽  
In Vitro Spermatogenesis ◽  
Review Study ◽  
Three Dimensional Culture

Introduction: Induction of in vitro spermatogenesis can be useful for infertility treatment in azoospermic patients and those undergoing chemotherapy. Different culture systems have been used to achieve this goal. This review study was performed with the aim to evaluate the application of 3D culture and testicular scaffolds in the establishment of in vitro spermatogenesis. In this review study, the information on the application of 3D culture and testicular scaffolds in induction of in vitro spermatogenesis was searched in databases such as SID, Magiran, PubMed, Irandoc, Iranmedx Scopus, Google Scholar, Web of Science using the keywords of three dimensional culture, testicular scaffold, spermatogenesis, spermatogonial stem cells without time limitation. Data analysis was carried out qualitatively. Finally, 35 papers in English and Persian were used to compile the article. In order to induce of in vitro spermatogenesis, three-dimensional culture methods such as testicular tissue culture, soft agar culture system, natural biomaterial scaffolds such as collagen, and scaffolds derived from decellularized testis have been used. Conclusion: Three-dimensional culture using spermatogonial stem cells and scaffolds can be used in vitro for induction of spermatogenesis, but there are further technical and ethical challenges in the path of fertile sperm production for the treatment of infertility.  

In vitro spermatogenesis by three-dimensional culture of rat testicular cells in collagen gel matrix

Biomaterials ◽  
2006 ◽  
Vol 27 (14) ◽  
pp. 2845-2853 ◽  
Author(s):  
Jae Ho Lee ◽  
Hyun Joo Kim ◽  
Haekwon Kim ◽  
Sang Jin Lee ◽  
Myung Chan Gye
Keyword(s):  
Three Dimensional ◽  
Collagen Gel ◽  
In Vitro Spermatogenesis ◽  
Testicular Cells ◽  
Three Dimensional Culture ◽  
Collagen Gel Matrix
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