scholarly journals Quantitative measurement of transcript levels throughout human preimplantation development: analysis of hypoxanthine phosphoribosyl transferase

2001 ◽  
Vol 7 (2) ◽  
pp. 147-154 ◽  
Author(s):  
D. M. Taylor
Keyword(s):  
Quantitative Measurement ◽  
Preimplantation Development ◽  
Hypoxanthine Phosphoribosyl Transferase ◽  
Transcript Levels ◽  
Phosphoribosyl Transferase ◽  
Development Analysis

HPRT activity in embryos of a South American opossum Monodelphis domestica

1994 ◽  
Vol 6 (4) ◽  
pp. 529 ◽  
Author(s):  
PG Johnston ◽  
D Dean ◽  
JL VandeBerg ◽  
ES Robinson
Keyword(s):  
X Chromosome ◽  
Blastocyst Stage ◽  
Monodelphis Domestica ◽  
X Inactivation ◽  
Hypoxanthine Phosphoribosyl Transferase ◽  
South American ◽  
Cell Stage ◽  
Phosphoribosyl Transferase ◽  
Stage Of Development ◽  
American Opossum

Marsupial females show preferential paternal X-inactivation. However, the time at which X-inactivation occurs in early development has not yet been determined. A double microassay which measures the activities of X-linked hypoxanthine phosphoribosyl transferase (HPRT) and the autosomally-coded adenine phosphoribosyl transferase (APRT) from the same sample was performed on a collection of embryos from a South American opossum Monodelphis domestica. The embryos ranged in age from the 2-cell stage to the bilaminar blastocyst stage. The results indicate that their embryonic HPRT and APRT are not expressed until just before the unilaminar blastocyst stage in M. domestica. This is at a later stage of development than that in the mouse where embryonic HPRT and APRT expression first occurs at the 4-8-cell stage. It is concluded that HPRT is an uniformative enzyme for assessing X chromosome activity in cleaving embryos of M. domestica. The widespread distribution of HPRT:APRT ratios after the unilaminar blastocyst stage also makes it difficult to draw conclusions about the state of X chromosome activity in early marsupial development.

Duplication in the hypoxanthine phosphoribosyl-transferase gene caused by Alu-Alu recombination in a patient with Lesch Nyhan syndrome

1993 ◽  
Vol 90 (5) ◽  
Author(s):  
Suzanne Marcus ◽  
Dennis Hellgren ◽  
Bo Lambert ◽  
SvenPetter F�llstr�m ◽  
Jan Wahlstr�m
Keyword(s):  
Hypoxanthine Phosphoribosyl Transferase ◽  
Phosphoribosyl Transferase ◽  
Transferase Gene
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