scholarly journals Molecular Characterization of a Diagnostic DNA Marker for Domesticated Tetraploid Wheat Provides Evidence for Gene Flow from Wild Tetraploid Wheat to Hexaploid Wheat

2006 ◽  
Vol 23 (7) ◽  
pp. 1386-1396 ◽  
Author(s):  
J. Dvorak
Keyword(s):  
Gene Flow ◽  
Molecular Characterization ◽  
Hexaploid Wheat ◽  
Dna Marker ◽  
Tetraploid Wheat

scholarly journals Phenotypic and molecular characterization of Hessian fly resistance in diploid wheat, Aegilops tauschii

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Jill A. Nemacheck ◽  
Brandon J. Schemerhorn ◽  
Steven R. Scofield ◽  
Subhashree Subramanyam
Keyword(s):  
Genetic Resources ◽  
Molecular Characterization ◽  
Hexaploid Wheat ◽  
Aegilops Tauschii ◽  
Hessian Fly ◽  
Functional Validation ◽  
Diploid Progenitor ◽  
Molecular Responses ◽  
Physical Measurements

Abstract Background The Hessian fly (Mayetiola destructor), belonging to the gall midge family (Cecidomyiidae), is a devastating pest of wheat (Triticum aestivum) causing significant yield losses. Despite identification and characterization of numerous Hessian fly-responsive genes and associated biological pathways involved in wheat defense against this dipteran pest, their functional validation has been challenging. This is largely attributed to the large genome, polyploidy, repetitive DNA, and limited genetic resources in hexaploid wheat. The diploid progenitor Aegilops tauschii, D-genome donor of modern-day hexaploid wheat, offers an ideal surrogate eliminating the need to target all three homeologous chromosomes (A, B and D) individually, and thereby making the functional validation of candidate Hessian fly-responsive genes plausible. Furthermore, the well-annotated sequence of Ae. tauschii genome and availability of genetic resources amenable to manipulations makes the functional assays less tedious and time-consuming. However, prior to utilization of this diploid genome for downstream studies, it is imperative to characterize its physical and molecular responses to Hessian fly. Results In this study we screened five Ae. tauschii accessions for their response to the Hessian fly biotypes L and vH13. Two lines were identified that exhibited a homozygous resistance response to feeding by both Hessian fly biotypes. Studies using physical measurements and neutral red staining showed that the resistant Ae. tauschii accessions resembled hexaploid wheat in their phenotypic responses to Hessian fly, that included similarities in larval developmental stages, leaf and plant growth, and cell wall permeability. Furthermore, molecular responses, characterized by gene expression profiling using quantitative real-time PCR, in select resistant Ae. tauschii lines also revealed similarities with resistant hexaploid wheat. Conclusions Phenotypic and molecular characterization of Ae. tauschii to Hessian fly infestation revealed resistant accessions that shared similarities to hexaploid wheat. Resembling the resistant hexaploid wheat, the Ae. tauschii accessions mount an early defense strategy involving defense proteins including lectins, secondary metabolites and reactive oxygen species (ROS) radicals. Our results reveal the suitability of the diploid progenitor for use as an ideal tool for functional genomics research in deciphering the wheat-Hessian fly molecular interactions.

scholarly journals Molecular Characterization of Rht-1 Dwarfing Genes in Hexaploid Wheat

2011 ◽  
Vol 157 (4) ◽  
pp. 1820-1831 ◽  
Author(s):  
Stephen Pearce ◽  
Robert Saville ◽  
Simon P. Vaughan ◽  
Peter M. Chandler ◽  
Edward P. Wilhelm ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Hexaploid Wheat ◽  
Dwarfing Genes

Molecular characterization of a gender-linked DNA marker and a related gene in Mercurialis annua L.

Planta ◽  
2005 ◽  
Vol 222 (6) ◽  
pp. 1063-1070 ◽  
Author(s):  
Deepak Kumar Khadka ◽  
Ali Nejidat ◽  
Moshe Tal ◽  
Avi Golan-Goldhirsh
Keyword(s):  
Molecular Characterization ◽  
Dna Marker ◽  
Related Gene ◽  
Mercurialis Annua

Molecular characterization of three GIBBERELLIN-INSENSITIVE DWARF1 homologous genes in hexaploid wheat

2013 ◽  
Vol 170 (4) ◽  
pp. 432-443 ◽  
Author(s):  
Aixia Li ◽  
Wenlong Yang ◽  
Shengjun Li ◽  
Dongcheng Liu ◽  
Xiaoli Guo ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Hexaploid Wheat ◽  
Homologous Genes

Molecular characterization of waxy alleles in three subspecies of hexaploid wheat and identification of two novel Wx-B1 alleles

2015 ◽  
Vol 128 (12) ◽  
pp. 2427-2435 ◽  
Author(s):  
Marcela Ayala ◽  
Juan B. Alvarez ◽  
Makoto Yamamori ◽  
Carlos Guzmán
Keyword(s):  
Molecular Characterization ◽  
Hexaploid Wheat

Comprehensive molecular characterization of the α/β-gliadin multigene family in hexaploid wheat

2015 ◽  
Vol 291 (1) ◽  
pp. 65-77 ◽  
Author(s):  
Satoshi Noma ◽  
Kanako Kawaura ◽  
Katsuyuki Hayakawa ◽  
Chikako Abe ◽  
Noritaka Tsuge ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Multigene Family ◽  
Hexaploid Wheat

scholarly journals Genetic and Molecular Characterization of the VRN2 Loci in Tetraploid Wheat

2008 ◽  
Vol 149 (1) ◽  
pp. 245-257 ◽  
Author(s):  
Assaf Distelfeld ◽  
Gabriela Tranquilli ◽  
Chengxia Li ◽  
Liuling Yan ◽  
Jorge Dubcovsky
Keyword(s):  
Molecular Characterization ◽  
Tetraploid Wheat
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