scholarly journals Evolution of Noncoding and Silent Coding Sites in the Plasmodium falciparum and Plasmodium reichenowi Genomes

2005 ◽  
Vol 22 (7) ◽  
pp. 1621-1626 ◽  
Author(s):  
Daniel E. Neafsey ◽  
Daniel L. Hartl ◽  
Matt Berriman
Keyword(s):  
Plasmodium Falciparum ◽  
Plasmodium Reichenowi

Genetic Distance in Housekeeping Genes Between Plasmodium falciparum and Plasmodium reichenowi and Within P. falciparum

2004 ◽  
Vol 59 (5) ◽  
pp. 687-694 ◽  
Author(s):  
Kazuyuki Tanabe ◽  
Naoko Sakihama ◽  
Tetsuya Hattori ◽  
Lisa Ranford-Cartwright ◽  
Ira Goldman ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Genetic Distance ◽  
Housekeeping Genes ◽  
Plasmodium Reichenowi

Sequence of a 27-kilodalton gamete antigen of Plasmodium reichenowi and comparison with Pfg27 of Plasmodium falciparum

1993 ◽  
Vol 59 (1) ◽  
pp. 175-176 ◽  
Author(s):  
Altaf A. Lal ◽  
Ira F. Goldman ◽  
William E. Collins ◽  
Nirbhay Kumar
Keyword(s):  
Plasmodium Falciparum ◽  
Plasmodium Reichenowi

scholarly journals VAR2CSA binding phenotype has ancient origin and arose before Plasmodium falciparum crossed to humans: implications in placental malaria vaccine design

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Stéphane Gangnard ◽  
Arnaud Chêne ◽  
Sébastien Dechavanne ◽  
Anand Srivastava ◽  
Marion Avril ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Birth Outcomes ◽  
Recombinant Proteins ◽  
Malaria Vaccine ◽  
Placental Malaria ◽  
Humoral Response ◽  
High Specificity ◽  
Parasite Populations ◽  
Antibody Epitopes ◽  
Plasmodium Reichenowi

AbstractVAR2CSA is a leading candidate for developing a placental malaria (PM) vaccine that would protect pregnant women living in malaria endemic areas against placental infections and improve birth outcomes. Two VAR2CSA-based PM vaccines are currently under clinical trials, but it is still unclear if the use of a single VAR2CSA variant will be sufficient to induce a broad enough humoral response in humans to cross-react with genetically diverse parasite populations. Additional immuno-focusing vaccine strategies may therefore be required to identify functionally conserved antibody epitopes in VAR2CSA. We explored the possibility that conserved epitopes could exist between VAR2CSA from the chimpanzee parasite Plasmodium reichenowi and Plasmodium falciparum sequences. Making use of VAR2CSA recombinant proteins originating from both species, we showed that VAR2CSA from P. reichenowi (Pr-VAR2CSA) binds to the placental receptor CSA with high specificity and affinity. Antibodies raised against Pr-VAR2CSA were able to recognize native VAR2CSA from different P. falciparum genotypes and to inhibit the interaction between CSA and P. falciparum-infected erythrocytes expressing different VAR2CSA variants. Our work revealed the existence of cross-species inhibitory epitopes in VAR2CSA and calls for pre-clinical studies assessing the efficacy of novel VAR2CSA-based cross-species boosting regimens.

Ultrastructural observations on the in vitro cytoadherence of Plasmodium falciparum infected red blood cells

1990 ◽  
Vol 48 (3) ◽  
pp. 914-915
Author(s):  
D.J.P. Ferguson ◽  
A.R. Berendt ◽  
J. Tansey ◽  
K. Marsh ◽  
C.I. Newbold
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Umbilical Vein ◽  
Cell Types ◽  
Amelanotic Melanoma ◽  
Cytoplasmic Process ◽  
Umbilical Vein Endothelial Cells

In human malaria, the most serious clinical manifestation is cerebral malaria (CM) due to infection with Plasmodium falciparum. The pathology of CM is thought to relate to the fact that red blood cells containing mature forms of the parasite (PRBC) cytoadhere or sequester to post capillary venules of various tissues including the brain. This in vivo phenomenon has been studied in vitro by examining the cytoadherence of PRBCs to various cell types and purified proteins. To date, three Ijiost receptor molecules have been identified; CD36, ICAM-1 and thrombospondin. The specific changes in the PRBC membrane which mediate cytoadherence are less well understood, but they include the sub-membranous deposition of electron-dense material resulting in surface deformations called knobs. Knobs were thought to be essential for cytoadherence, lput recent work has shown that certain knob-negative (K-) lines can cytoadhere. In the present study, we have used electron microscopy to re-examine the interactions between K+ PRBCs and both C32 amelanotic melanoma cells and human umbilical vein endothelial cells (HUVEC).We confirm previous data demonstrating that C32 cells possess numerous microvilli which adhere to the PRBC, mainly via the knobs (Fig. 1). In contrast, the HUVEC were relatively smooth and the PRBCs appeared partially flattened onto the cell surface (Fig. 2). Furthermore, many of the PRBCs exhibited an invagination of the limiting membrane in the attachment zone, often containing a cytoplasmic process from the endothelial cell (Fig. 2).

Sign in / Sign up

Export Citation Format

Share Document