scholarly journals Yeast identification by sequencing, biochemical kits, MALDI–TOF MS and rep-PCR DNA fingerprinting

2017 ◽  
Vol 56 (7) ◽  
pp. 816-827 ◽  
Author(s):  
Ying Zhao ◽  
Chi-Ching Tsang ◽  
Meng Xiao ◽  
Jasper F W Chan ◽  
Susanna K P Lau ◽  
...  
Keyword(s):  
Dna Fingerprinting ◽  
Yeast Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Development and Validation of an in-House Library of Colombian Candida auris Strains with MALDI-TOF MS to Improve Yeast Identification

2020 ◽  
Vol 6 (2) ◽  
pp. 72 ◽  
Author(s):  
Andrés Ceballos-Garzon ◽  
Daniela Amado ◽  
Norida Vélez ◽  
María José Jiménez-A ◽  
Crescencio Rodríguez ◽  
...  
Keyword(s):  
Mass Spectra ◽  
Candida Auris ◽  
Yeast Identification ◽  
Great Opportunity ◽  
Maldi Tof Ms ◽  
Identification Process ◽  
Clinical Strains ◽  
Maldi Tof ◽  
Development And Validation ◽  
Tof Ms

Background: Candida auris is characterized for having a high genetic variability among species. MALDI-TOF MS library contains spectra from only three strains of C. auris, which makes difficult the identification process and gives low scores at the species level. Our aim was to construct and validate an internal library to improve C. auris identification with Colombian clinical strains. Methods: From 30 clinical strains, 770 mass spectra were obtained for the construction of the database. The validation was performed with 300 strains to compare the identification results in the BDAL and C. auris Colombia libraries. Results: Our library allowed a complete, 100% identification of the evaluated strains and a significant improvement in the scores obtained, showing a better performance compared to the Bruker BDAL library. Conclusions: The strengthening of the database is a great opportunity to improve the scoring and C. auris identification. Library data are available via ProteomeXchange with identifier PXD016387.

scholarly journals Evaluation of four pretreatment procedures for MALDI-TOF MS yeast identification in the routine clinical laboratory

2013 ◽  
Vol 51 (4) ◽  
pp. 371-377 ◽  
Author(s):  
Carole Cassagne ◽  
Anne-Laure Cella ◽  
Pierre Suchon ◽  
Anne-Cecile Normand ◽  
Stephane Ranque ◽  
...  
Keyword(s):  
Clinical Laboratory ◽  
Yeast Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Routine Clinical Laboratory ◽  
Tof Ms

Development and validation of an extended database for yeast identification by MALDI-TOF MS in Argentina

2018 ◽  
Vol 57 (2) ◽  
pp. 215-225 ◽  
Author(s):  
Constanza Giselle Taverna ◽  
Mariana Mazza ◽  
Nadia Soledad Bueno ◽  
Christian Alvarez ◽  
Susana Amigot ◽  
...  
Keyword(s):  
Yeast Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Development And Validation ◽  
Tof Ms

scholarly journals Population Dynamics and Yeast Diversity in Early Winemaking Stages without Sulfites Revealed by Three Complementary Approaches

2021 ◽  
Vol 11 (6) ◽  
pp. 2494
Author(s):  
Sara Windholtz ◽  
Lucie Dutilh ◽  
Marine Lucas ◽  
Julie Maupeu ◽  
Amélie Vallet-Courbin ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Protein Extraction ◽  
Wine Yeast ◽  
Yeast Identification ◽  
Maldi Tof Ms ◽  
Operational Taxonomic Units ◽  
Maldi Tof ◽  
Hanseniaspora Uvarum ◽  
Standard Procedures ◽  
Tof Ms

Nowadays, the use of sulfur dioxide (SO2) during the winemaking process is a controversial societal issue. In order to reduce its use, various alternatives are emerging, in particular bioprotection by adding yeasts, with different impacts on yeast microbiota in early winemaking stages. In this study, quantitative-PCR and metabarcoding high-throughput sequencing (HTS) were combined with MALDI-TOF-MS to monitor yeast population dynamic and diversity in the early stages of red winemaking process without sulfites and with bioprotection by Torulaspora delbrueckii and Metschnikowia pulcherrima addition. By using standard procedures for yeast protein extraction and a laboratory-specific database of wine yeasts, identification at species level of 95% of the isolates was successfully achieved by MALDI-TOF-MS, thus confirming that it is a promising method for wine yeast identification. The different approaches confirmed the implantation and the niche occupation of bioprotection leading to the decrease of fungal communities (HTS) and Hanseniaspora uvarum cultivable population (MALDI-TOF MS). Yeast and fungi diversity was impacted by stage of maceration and, to a lesser extent, by bioprotection and SO2, resulting in a modification of the nature and abundance of the operational taxonomic units (OTUs) diversity.

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

HPTLC-MALDI/TOF/MS vs. HPLC-ESI/MS2 for identification to flavonoids in plant extracts

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
L Fougère ◽  
D Da Silva ◽  
E Destandau ◽  
C Elfakir
Keyword(s):  
Plant Extracts ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Infektionserreger beim Schwein – Analyse des kultivierbaren Mikrobioms mittels MALDI-TOF MS

2017 ◽  
Author(s):  
M Erhard ◽  
M Metzner ◽  
D Köhler-Repp ◽  
B Köhler ◽  
R Storandt
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Quantitative metabolomics of Iranian saffron based on their HPLC-DAD and MALDI-TOF-MS

2019 ◽  
Author(s):  
M Hooshyari ◽  
H Rezadoost ◽  
P Ghezellou ◽  
A Ghassempour
Keyword(s):  
Maldi Tof Ms ◽  
Quantitative Metabolomics ◽  
Maldi Tof ◽  
Tof Ms
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