scholarly journals Elicitors from the endophytic fungus Trichoderma atroviride promote Salvia miltiorrhiza hairy root growth and tanshinone biosynthesis

2013 ◽  
Vol 64 (18) ◽  
pp. 5687-5694 ◽  
Author(s):  
Qianliang Ming ◽  
Chunyan Su ◽  
Chengjian Zheng ◽  
Min Jia ◽  
Qiaoyan Zhang ◽  
...  
Keyword(s):  
Root Growth ◽  
Hairy Root ◽  
Endophytic Fungus ◽  
Salvia Miltiorrhiza ◽  
Trichoderma Atroviride

Tanshinone IIA and tanshinone I production by Trichoderma atroviride D16, an endophytic fungus in Salvia miltiorrhiza

Phytomedicine ◽  
2012 ◽  
Vol 19 (3-4) ◽  
pp. 330-333 ◽  
Author(s):  
Qianliang Ming ◽  
Ting Han ◽  
Wenchao Li ◽  
Qiaoyan Zhang ◽  
Hong Zhang ◽  
...  
Keyword(s):  
Endophytic Fungus ◽  
Salvia Miltiorrhiza ◽  
Tanshinone Iia ◽  
Trichoderma Atroviride ◽  
Tanshinone I

Effects of Streptomyces pactum Act12 on Salvia miltiorrhiza Hairy Root Growth and Tanshinone Synthesis and Its Mechanisms

2014 ◽  
Vol 173 (4) ◽  
pp. 883-893 ◽  
Author(s):  
Yan Yan ◽  
Shuncang Zhang ◽  
Dongfeng Yang ◽  
Jiayi Zhang ◽  
Zongsuo Liang
Keyword(s):  
Root Growth ◽  
Hairy Root ◽  
Salvia Miltiorrhiza ◽  
Streptomyces Pactum

scholarly journals Endophyte Chaetomium globosum D38 and its elicitors promote tanshinones accumulation of Salvia miltiorrhiza

2017 ◽  
Author(s):  
Xin Zhai ◽  
Dong Luo ◽  
Xiuqing Li ◽  
Ting Han ◽  
Zhouyang Kong ◽  
...  
Keyword(s):  
Hairy Root ◽  
Endophytic Fungi ◽  
Endophytic Fungus ◽  
Salvia Miltiorrhiza ◽  
Chaetomium Globosum ◽  
Primary Metabolism ◽  
Phenylpropionic Acid ◽  
Host Growth ◽  
Acid Accumulation ◽  
Plant Tissue Cultures

AbstractDue to the low yield of tanshinones and their analogues in Salvia miltiorrhiza, there are all kinds of stimulation strategies having been applied to improve tanshinones output in plant tissue cultures. Endophytic fungi have formed various different relationships with their host plants withstanding host and environmental factors, including symbiotic, mutualistic, commensalistic, and parasitic. Thus we take the assumption that endophytic fungi may be an emerging microbial tool used to promote secondary metabolism, which will promote the production of active compounds through endophyte-based biology method. Our study therefore aimed to examine the effects of live endophytic fungus Chaetomium globosum D38 and its elicitors on the accumulation of tanshinones in hairy root cultures of Salvia miltiorrhiza. C. globosum D38 mainly colonized in the intercellular gap of xylem parenchyma cells of S. miltiorrhiza hairy root, during long term co-existence without any toxicity against S. miltiorrhiza hairy root. Moreover, both of the live fungus and its mycelia extracts could induce the production of tanshinones, in special dihydrotanshinone I and cryptotanshinone. The effects of mycelia extracts were much stronger than that of the live fungus on tanshinones synthesis, which increased the transcriptional activity of genes with repect to tanshinone biosynthetic pathway obviously. Our results indicated that both of the live C. globosum D38 and its mycelia extracts could be utilized for tanshinones accumulation in S. miltiorrhiza hairy root. What’s more, D38 also could be made into biotic fertilizer applying into S.miltiorrhiza seddlings, which not only promoted host growth but the tanshinones and phenylpropionic acid accumulation. In the soil environment, D38 had formed bitrophic and mutual beneficial relationship with the host and enhanced the primary metabolism on the whole so as to have facilitative effects on phenylpropionic acid accumulation. To sum up, Chaetomium globosum D38 was a highly effective endophytic fungus for S. miltiorrhiza.

scholarly journals UHPLC-HRMSn Analysis Reveals the Dynamic Metabonomic Responses of Salvia miltiorrhiza Hairy Roots to Polysaccharide Fraction from Trichoderma atroviride

Biomolecules ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 541 ◽  
Author(s):  
Ming ◽  
Dong ◽  
Wu ◽  
Zhu ◽  
Jia ◽  
...  
Keyword(s):  
Hairy Root ◽  
Hairy Roots ◽  
Clustering Analysis ◽  
Metabolic Profiling ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Salvia Miltiorrhiza ◽  
Principal Component ◽  
Trichoderma Atroviride ◽  
Polysaccharide Fraction

We have previously reported that Trichoderma atroviride, an endophytic fungus isolated from S. miltiorrhiza, promotes S. miltiorrhiza hairy root growth and significantly stimulates the biosynthesis of tanshinones specifically the polysaccharide fraction (PSF). However, this study only focused exclusively on six metabolites whilst ignoring changes to the whole metabolite composition of the S. miltiorrhiza hairy roots. In the present study, the dynamic metabonomic responses of S. miltiorrhiza hairy roots were investigated using ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMSn). UHPLC-HRMS typical total ions chromatograms (TICs) of PSF-treated hairy root samples were different from the control. Moreover, the results of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) indicated that PSF-treated samples were significantly different from the control. Through the analysis of PLS-DA, a total of 114 and 99 differential metabolites were found from the positive and negative models respectively and a total of 33 differential metabolites were identified. Thus, S. miltiorrhiza hairy roots had been induced to regulate the metabolic profiling in response to PSF and the changes of the metabolic profiling contributed to promoting the biosynthesis of tanshinones notably whilst the biosynthesis of phenolic acids were slightly inhibited.

scholarly journals La Dramaticaly Enhances the Accumulation of Tanshinones in Salvia Miltiorrhiza Hairy Root Cultures

2012 ◽  
Vol 2 (1) ◽  
Author(s):  
Jie Zhou ◽  
Lei Fang ◽  
Xiao Wang ◽  
Lanping Guo ◽  
Luqi Huang
Keyword(s):  
Hairy Root ◽  
Salvia Miltiorrhiza ◽  
Hairy Root Cultures ◽  
Root Cultures
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