scholarly journals Molecular cloning and characterization of PtrLAR3, a gene encoding leucoanthocyanidin reductase from Populus trichocarpa, and its constitutive expression enhances fungal resistance in transgenic plants

2012 ◽  
Vol 63 (7) ◽  
pp. 2513-2524 ◽  
Author(s):  
Li Yuan ◽  
Lijun Wang ◽  
Zujing Han ◽  
Yuanzhong Jiang ◽  
Lili Zhao ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Molecular Cloning ◽  
Populus Trichocarpa ◽  
Constitutive Expression ◽  
Fungal Resistance ◽  
Gene Encoding ◽  
Leucoanthocyanidin Reductase

Molecular cloning and characterization of SoNCED, a novel gene encoding 9-cis-epoxycarotenoid dioxygenase from sugarcane (Saccharum officinarum L.)

2013 ◽  
Vol 35 (1) ◽  
pp. 101-109 ◽  
Author(s):  
Chang-Ning Li ◽  
Manoj-Kumar Srivastava ◽  
Qian Nong ◽  
Li-Tao Yang ◽  
Yang-Rui Li
Keyword(s):  
Molecular Cloning ◽  
Saccharum Officinarum ◽  
Gene Encoding ◽  
Novel Gene

scholarly journals Molecular cloning and characterization of a gene encoding the proline transporter protein in common bean ( Phaseolus vulgaris L.)

2016 ◽  
Vol 4 (5) ◽  
pp. 384-390 ◽  
Author(s):  
Jibao Chen ◽  
Jing Wu ◽  
Yunfeng Lu ◽  
Yuannan Cao ◽  
Hui Zeng ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Molecular Cloning ◽  
Phaseolus Vulgaris L ◽  
Gene Encoding ◽  
Transporter Protein

scholarly journals Molecular Cloning and Characterization of a Novel Gene Encoding DREB Protein from Buchloe dactyloides (Nutt.) Engelm

2011 ◽  
Vol 4 (1) ◽  
Author(s):  
Yan Sun ◽  
Yan Li ◽  
Xiaoyan Hu ◽  
Qingchuan Yang ◽  
Junmei Kang ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Buchloe Dactyloides ◽  
Gene Encoding ◽  
Novel Gene

scholarly journals Functional Cloning and Expression of the Schizophyllum commune Glucuronoyl Esterase Gene and Characterization of the Recombinant Enzyme

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Dominic W. S. Wong ◽  
Victor J. Chan ◽  
Amanda A. McCormack ◽  
Ján Hirsch ◽  
Peter Biely
Keyword(s):  
Glucuronic Acid ◽  
Active Form ◽  
Schizophyllum Commune ◽  
Constitutive Expression ◽  
Recombinant Enzyme ◽  
Cloning And Expression ◽  
Gene Encoding ◽  
Glucuronoyl Esterase ◽  
Esterase Gene

The gene encoding Schizophyllum commune glucuronoyl esterase was identified in the scaffold 17 of the genome, containing two introns of 50 bp and 48 bp, with a transcript sequence of 1179 bp. The gene was synthesized and cloned into Pichia pastoris expression vector pGAPZα to achieve constitutive expression and secretion of the recombinant enzyme in soluble active form. The purified protein was 53 kD with glycosylation and had an acidic pI of 3.7. Activity analysis on several uronic acids and their derivatives suggests that the enzyme recognized only esters of 4-O-methyl-D-glucuronic acid derivatives, even with a 4-nitrophenyl aglycon but did not hydrolyze the ester of D-galacturonic acid. The kinetic values were Km 0.25 mM, Vmax 16.3 μM⋅min−1, and kcat 9.27 s−1 with 4-nitrophenyl 2-O-(methyl 4-O-methyl-α-D-glucopyranosyluronate)-β-D-xylopyranoside as the substrate.

Sign in / Sign up

Export Citation Format

Share Document