scholarly journals A comparison of the low temperature transcriptomes and CBF regulons of three plant species that differ in freezing tolerance: Solanum commersonii, Solanum tuberosum, and Arabidopsis thaliana

2011 ◽  
Vol 62 (11) ◽  
pp. 3807-3819 ◽  
Author(s):  
Marcela A. Carvallo ◽  
María-Teresa Pino ◽  
Zoran Jeknić ◽  
Cheng Zou ◽  
Colleen J. Doherty ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Solanum Tuberosum ◽  
Low Temperature ◽  
Plant Species ◽  
Freezing Tolerance ◽  
Solanum Commersonii

scholarly journals Metabolic and Genomic Traits of Phytobeneficial Phenazine-Producing Pseudomonas spp. Are Linked to Rhizosphere Colonization in Arabidopsis thaliana and Solanum tuberosum

2019 ◽  
Vol 86 (4) ◽  
Author(s):  
Antoine Zboralski ◽  
Adrien Biessy ◽  
Marie-Claude Savoie ◽  
Amy Novinscak ◽  
Martin Filion
Keyword(s):  
Arabidopsis Thaliana ◽  
Solanum Tuberosum ◽  
Plant Species ◽  
Growth Promotion ◽  
Screening Tests ◽  
Taqman Probe ◽  
Phenotype Microarray ◽  
Content Type ◽  
Rhizosphere Colonization ◽  
Colonization Process

ABSTRACT Bacterial rhizosphere colonization is critical for phytobeneficial rhizobacteria such as phenazine-producing Pseudomonas spp. To better understand this colonization process, potential metabolic and genomic determinants required for rhizosphere colonization were identified using a collection of 60 phenazine-producing Pseudomonas strains isolated from multiple plant species and representative of the worldwide diversity. Arabidopsis thaliana and Solanum tuberosum (potato) were used as host plants. Bacterial rhizosphere colonization was measured by quantitative PCR using a newly designed primer pair and TaqMan probe targeting a conserved region of the phenazine biosynthetic operon. The metabolic abilities of the strains were assessed on 758 substrates using Biolog phenotype microarray technology. These data, along with available genomic sequences for all strains, were analyzed in light of rhizosphere colonization. Strains belonging to the P. chlororaphis subgroup colonized the rhizospheres of both plants more efficiently than strains belonging to the P. fluorescens subgroup. Metabolic results indicated that the ability to use amines and amino acids was associated with an increase in rhizosphere colonization capability in A. thaliana and/or in S. tuberosum. The presence of multiple genetic determinants in the genomes of the different strains involved in catabolic pathways and plant-microbe and microbe-microbe interactions correlated with increased or decreased rhizosphere colonization capabilities in both plants. These results suggest that the metabolic and genomic traits found in different phenazine-producing Pseudomonas strains reflect their rhizosphere competence in A. thaliana and S. tuberosum. Interestingly, most of these traits are associated with similar rhizosphere colonizing capabilities in both plant species. IMPORTANCE Rhizosphere colonization is crucial for plant growth promotion and biocontrol by antibiotic-producing Pseudomonas spp. This colonization process relies on different bacterial determinants which partly remain to be uncovered. In this study, we combined a metabolic and a genomic approach to decipher new rhizosphere colonization determinants which could improve our understanding of this process in Pseudomonas spp. Using 60 distinct strains of phenazine-producing Pseudomonas spp., we show that rhizosphere colonization abilities correlated with both metabolic and genomic traits when these bacteria were inoculated on two distant plants, Arabidopsis thaliana and Solanum tuberosum. Key metabolic and genomic determinants presumably required for efficient colonization of both plant species were identified. Upon further validation, these targets could lead to the development of simple screening tests to rapidly identify efficient rhizosphere colonizers.

scholarly journals Evolutionary history of the C-repeat binding factor/dehydration-responsive element-binding 1 (CBF/DREB1) protein family in 43 plant species and characterization of CBF/DREB1 proteins in Solanum tuberosum

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Wan Li ◽  
Yue Chen ◽  
Minghui Ye ◽  
Haibin Lu ◽  
Dongdong Wang ◽  
...  
Keyword(s):  
Heat Stress ◽  
Solanum Tuberosum ◽  
High Temperature ◽  
Low Temperature ◽  
Plant Species ◽  
Growth And Development ◽  
Protein Family ◽  
Temperature Stresses ◽  
Binding Factor ◽  
Responsive Element

Abstract Background Plants are easily affected by temperature variations, and high temperature (heat stress) and low temperature (cold stress) will lead to poor plant development and reduce crop yields. Therefore, it is very important to identify resistance genes for improving the ability of plants to resist heat stress or cold stress by using modern biotechnology. Members of the C-repeat binding factor/Dehydration responsive element-binding 1 (CBF/DREB1) protein family are related to the stress resistance of many plant species. These proteins affect the growth and development of plants and play vital roles during environmental stress (cold, heat, drought, salt, etc.). In this study, we identified CBF/DREB1 genes from 43 plant species (including algae, moss, ferns, gymnosperms, angiosperms) by using bioinformatic methods to clarify the characteristics of the CBF/DREB1 protein family members and their functions in potato under heat and cold stresses. Results In this study, we identified 292 CBF/DREB1 proteins from 43 plant species. However, no CBF/DREB1 protein was found in algae, moss, ferns, or gymnosperms; members of this protein family exist only in angiosperms. Phylogenetic analysis of all the CBF/DREB1 proteins revealed five independent groups. Among them, the genes of group I do not exist in eudicots and are found only in monocots, indicating that these genes have a special effect on monocots. The analysis of motifs, gene duplication events, and the expression data from the PGSC website revealed the gene structures, evolutionary relationships, and expression patterns of the CBF/DREB1 proteins. In addition, analysis of the transcript levels of the 8 CBF/DREB1 genes in potato (Solanum tuberosum) under low-temperature and high-temperature stresses showed that these genes were related to temperature stresses. In particular, the expression levels of StCBF3 and StCBF4 in the leaves, stems, and roots significantly increased under high-temperature conditions, which suggested that StCBF3 and StCBF4 may be closely related to heat tolerance in potato. Conclusion Overall, members of the CBF/DREB1 protein family exist only in angiosperms and plays an important role in the growth and development of plants. In addition, the CBF/DREB1 protein family is related to the heat and cold resistance of potato. Our research revealed the evolution of the CBF/DREB1 family, and is useful for studying the precise functions of the CBF/DREB1 proteins when the plants are developing and are under temperature stress.

Induction of freezing tolerance in potato (Solanum commersonii) suspension cultured cells

1992 ◽  
Vol 84 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Stephen P. Lee ◽  
Baolong Zhu ◽  
Tony H. H. Chen ◽  
Paul H. Li
Keyword(s):  
Freezing Tolerance ◽  
Cultured Cells ◽  
Solanum Commersonii ◽  
Suspension Cultured Cells
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