scholarly journals Isolation and expression analysis of genes encoding MET, CMT, and DRM methyltransferases in oil palm (Elaeis guineensis Jacq.) in relation to the ‘mantled’ somaclonal variation

2008 ◽  
Vol 59 (12) ◽  
pp. 3271-3281 ◽  
Author(s):  
Alain Rival ◽  
Estelle Jaligot ◽  
Thierry Beulé ◽  
E. Jean Finnegan
2000 ◽  
Vol 19 (7) ◽  
pp. 684-690 ◽  
Author(s):  
E. Jaligot ◽  
A. Rival ◽  
T. Beulé ◽  
S. Dussert ◽  
J.-L. Verdeil

Genome ◽  
2004 ◽  
Vol 47 (1) ◽  
pp. 224-228 ◽  
Author(s):  
E Jaligot ◽  
T Beulé ◽  
F-C Baurens ◽  
N Billotte ◽  
A Rival

The methylation-sensitive amplification polymorphism (MSAP) technique has been employed on somatic embryo-derived oil palms (Elaeis guineensis Jacq.) to identify methylation polymorphisms correlated with the "mantled" somaclonal variation. The variant phenotype displays an unstable feminization of male organs in both male and female flowers. Using MSAP, the methylation status of CCGG sites was compared in three normal versus three mantled regenerants sampled in clonal populations obtained through somatic embryogenesis from four genotypically distinct mother palms. Overall, 64 selective primer combinations were used and they have amplified 23 markers exhibiting a differential methylation pattern between the two phenotypes. Our results indicate that CCGG sites are poorly affected by the considerable decrease in global DNA methylation that has been previously associated with the mantled phenotype. Each of the 23 markers isolated in the present study could discriminate between the two phenotypes only when they were from the same genetic origin. This result hampers at the moment the direct use of MSAP markers for the early detection of variants, even though valuable information on putative target sequences will be obtained from a further characterization of these polymorphic markers.Key words: DNA methylation, epigenetics, MSAP, oil palm, somaclonal variation.


Plant Omics ◽  
2017 ◽  
Vol 10 (05) ◽  
pp. 247-251 ◽  
Author(s):  
Yurnaliza ◽  
◽  
Rizkita Rachmi Esyanti ◽  
Agus Susanto ◽  
I Nyoman Pugeg Aryantha ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document