scholarly journals A new self-compatibility haplotype in the sweet cherry 'Kronio', S5', attributable to a pollen-part mutation in the SFB gene

2007 ◽  
Vol 58 (15-16) ◽  
pp. 4347-4356 ◽  
Author(s):  
A. Marchese ◽  
R. I. Boskovic ◽  
T. Caruso ◽  
A. Raimondo ◽  
M. Cutuli ◽  
...  
2020 ◽  
Vol 184 (4) ◽  
pp. 1702-1716
Author(s):  
Yang Li ◽  
Xuwei Duan ◽  
Chuanbao Wu ◽  
Jie Yu ◽  
Chunsheng Liu ◽  
...  

2018 ◽  
Vol 59 (6) ◽  
pp. 1265-1275 ◽  
Author(s):  
Kentaro Ono ◽  
Takashi Akagi ◽  
Takuya Morimoto ◽  
Ana W�nsch ◽  
Ryutaro Tao

1998 ◽  
pp. 249-254
Author(s):  
R. BoÅ¡kovic ◽  
K.R. Tobutt ◽  
K. Russell

HortScience ◽  
2000 ◽  
Vol 35 (6) ◽  
pp. 1121-1123 ◽  
Author(s):  
Ryutaro Tao ◽  
Tsuyoshi Habu ◽  
Hisayo Yamane ◽  
Akira Sugiura ◽  
Kazuya Iwamoto

Self-compatible cultivars of Japanese apricot (Prunus mume Sieb. et Zucc.) have a horticultural advantage over self-incompatible ones because no pollinizer is required. Self-incompatibility is gametophytic, as in other Prunus species. We searched for molecular markers to identify self-compatible cultivars based on the information about S-ribonucleases (S-RNases) of other Prunus species. Total DNA isolated from five self-incompatible and six self-compatible cultivars were PCR-amplified by oligonucleotide primers designed from conserved regions of Prunus S-RNases. Self-compatible cultivars exhibited a common band of ≈1.5 kbp. Self-compatible cultivars also showed a common band of ≈12.1 kbp when genomic DNA digested with HindIII was probed with the cDNA encoding S2-RNase of sweet cherry (Prunus avium L.). These results suggest that self-compatible cultivars of Japanese apricot have a common S-RNase allele that can be used as a molecular marker for self-compatibility.


2005 ◽  
Vol 130 (6) ◽  
pp. 893-898 ◽  
Author(s):  
Santiago Vilanova ◽  
Carlos Romero ◽  
Gerardo Llácer ◽  
María Luisa Badenes ◽  
Lorenzo Burgos

This report shows the PCR-based identification of the eight known self-(in)compatibility alleles (S1 to S7 and Sc) of apricot (Prunus armeniaca L.). Two sets of consensus primers, designed from P. armeniaca S-RNase genomic sequences and sweet cherry (P. avium L.) S-RNase-cDNAs, were used to amplify fragments containing the first and the second S-RNase intron, respectively. When the results obtained from the two PCRs were combined, all S-alleles could be distinguished. The identity of the amplified S-alleles was verified by sequencing the first intron and 135 base pairs (bp) of the second exon. The deduced amino acid sequences of these fragments showed the presence of the C1 and C2 Prunus L. S-RNase conserved regions. These results allowed us to confirm S-genotypes previously assigned by stylar ribonuclease analyses and to propose one self-(in)compatibility group (I) and one universal donor group (O) containing unique S-genotypes and self-compatible cultivars (SC). This PCR-based typing system also facilitates the identification of the Sc-allele and might be a very useful tool for predicting self-compatibility in apricot breeding progenies.


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