scholarly journals Expression of genes encoding cell wall modifying enzymes is induced by cold storage and reflects changes in pear fruit texture

2005 ◽  
Vol 56 (418) ◽  
pp. 2029-2036 ◽  
Author(s):  
Sandra Fonseca ◽  
Lurdes Monteiro ◽  
Maria G. Barreiro ◽  
Maria S. Pais
Keyword(s):  
Cell Wall ◽  
Cold Storage ◽  
Pear Fruit ◽  
Fruit Texture ◽  
Expression Of Genes ◽  
Genes Encoding

scholarly journals The MAP kinase TVK1 regulates conidiation, hydrophobicity and the expression of genes encoding cell wall proteins in the fungus Trichoderma virens

Microbiology ◽  
2007 ◽  
Vol 153 (7) ◽  
pp. 2137-2147 ◽  
Author(s):  
Artemio Mendoza-Mendoza ◽  
Teresa. Rosales-Saavedra ◽  
Carlos. Cortés ◽  
Verónica. Castellanos-Juárez ◽  
Pedro. Martínez ◽  
...  
Keyword(s):  
Cell Wall ◽  
Map Kinase ◽  
Cell Wall Proteins ◽  
Trichoderma Virens ◽  
Expression Of Genes ◽  
Genes Encoding

scholarly journals GABA Accumulation Causes Cell Elongation Defects and a Decrease in Expression of Genes Encoding Secreted and Cell Wall-Related Proteins in Arabidopsis thaliana

2011 ◽  
Vol 52 (5) ◽  
pp. 894-908 ◽  
Author(s):  
Hugues Renault ◽  
Abdelhak El Amrani ◽  
Ravishankar Palanivelu ◽  
Emily P. Updegraff ◽  
Agnès Yu ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Cell Elongation ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Gaba Accumulation ◽  
Related Proteins

scholarly journals Transcriptome and Functional Analysis of the Eukaryotic-Type Serine/Threonine Kinase PknB in Staphylococcus aureus

2009 ◽  
Vol 191 (13) ◽  
pp. 4056-4069 ◽  
Author(s):  
Stefanie Donat ◽  
Karin Streker ◽  
Tanja Schirmeister ◽  
Sonja Rakette ◽  
Thilo Stehle ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Biochemical Characterization ◽  
Dependent Manner ◽  
Serine Threonine Kinase ◽  
Expression Of Genes ◽  
Biochemical Assays ◽  
Genes Encoding ◽  
Regulatory Impact ◽  
Glutamine Synthesis

ABSTRACT The function of the Staphylococcus aureus eukaryotic-like serine/threonine protein kinase PknB was investigated by performing transcriptome analysis using DNA microarray technology and biochemical assays. The transcriptional profile revealed a strong regulatory impact of PknB on the expression of genes encoding proteins which are involved in purine and pyrimidine biosynthesis, cell wall metabolism, autolysis, and glutamine synthesis. Functional activity of overexpressed and purified PknB kinase was demonstrated using the myelin basic protein as a surrogate substrate. Phosphorylation occurred in a time-dependent manner with Mn2+ as a preferred cofactor. Furthermore, biochemical characterization revealed regulation of adenylosuccinate synthase (PurA) activity by phosphorylation. Phosphorylated PurA showed a 1.8-fold decrease in enzymatic activity compared to unphosphorylated PurA. Loss of PknB led to formation of larger cell clusters, and a pknB deletion strain showed 32-fold-higher sensitivity to the cell wall-active antibiotic tunicamycin. The results of this study strongly indicate that PknB has a role in regulation of purine biosynthesis, autolysis, and central metabolic processes in S. aureus.

scholarly journals Fruit Ripening in Sour Cherry: Changes in Expression of Genes Encoding Expansins and other Cell-wall-modifying Enzymes

2003 ◽  
Vol 128 (1) ◽  
pp. 16-22 ◽  
Author(s):  
Sang-Dong Yoo ◽  
Zhifang Gao ◽  
Claudio Cantini ◽  
Wayne H. Loescher ◽  
Steven van Nocker
Keyword(s):  
Cell Wall ◽  
Fruit Ripening ◽  
Pectate Lyase ◽  
Sour Cherry ◽  
Xyloglucan Endotransglycosylase ◽  
Model Species ◽  
Cell Wall Modification ◽  
Fruit Species ◽  
Expression Of Genes ◽  
Genes Encoding

A preliminary understanding of developmental processes among divergent species is essential to evaluate the applicability of information from model species to plants of agricultural importance. In tomato (Lycopersicon esculentum Mill.), where the molecular biology associated with fruit ripening has been studied most extensively, tissue softening is due at least in part to the activity of proteins called expansins, in concert with enzymatic activities that modify the pectin and xyloglucan components of the cell wall. We evaluated the potential for the concerted action of expansins and other cell wall-modifying enzymes during ripening in a highly divergent fruit species, sour cherry (Prunus cerasus L.). We identified a family of four expansin genes that was strongly upregulated at the advent of ripening. Activation of these genes was accompanied by strong upregulation of gene(s) encoding potential pectin methylesterases, pectate lyase(s), and xyloglucan endotransglycosylase(s). Initiation of ripening and gene induction were also associated with a rapid decrease in cell wall weight. These results suggest that expansin and several other distinct activities could be involved in ripening-associated cell wall modification in cherries.

scholarly journals Xylan Utilization Regulon in Xanthomonas citri pv. citri Strain 306: Gene Expression and Utilization of Oligoxylosides

2015 ◽  
Vol 81 (6) ◽  
pp. 2163-2172 ◽  
Author(s):  
V. Chow ◽  
D. Shantharaj ◽  
Y. Guo ◽  
G. Nong ◽  
G. V. Minsavage ◽  
...  
Keyword(s):  
Cell Wall ◽  
Citrus Canker ◽  
Glycoside Hydrolase Family ◽  
In Planta ◽  
Xanthomonas Citri ◽  
Plant Host ◽  
Gene Encoding ◽  
Content Type ◽  
Expression Of Genes ◽  
Genes Encoding

ABSTRACTXanthomonas citripv. citri strain 306 (Xcc306), a causative agent of citrus canker, produces endoxylanases that catalyze the depolymerization of cell wall-associated xylans. In the sequenced genomes of all plant-pathogenic xanthomonads, genes encoding xylanolytic enzymes are clustered in three adjacent operons. InXcc306, these consecutive operons contain genes encoding the glycoside hydrolase family 10 (GH10) endoxylanases Xyn10A and Xyn10C, theagu67gene, encoding a GH67 α-glucuronidase (Agu67), thexyn43Egene, encoding a putative GH43 α-l-arabinofuranosidase, and thexyn43Fgene, encoding a putative β-xylosidase. Recombinant Xyn10A and Xyn10C convert polymeric 4-O-methylglucuronoxylan (MeGXn) to oligoxylosides methylglucuronoxylotriose (MeGX3), xylotriose (X3), and xylobiose (X2).Xcc306 completely utilizes MeGXnpredigested with Xyn10A or Xyn10C but shows little utilization of MeGXn.Xcc306 with a deletion in the gene encoding α-glucuronidase (Xcc306 Δagu67) will not utilize MeGX3for growth, demonstrating the role of Agu67 in the complete utilization of GH10-digested MeGXn. Preferential growth on oligoxylosides compared to growth on polymeric MeGXnindicates that GH10 xylanases, either secreted byXcc306in plantaor produced by the plant host, generate oligoxylosides that are processed by Xyn10 xylanases and Agu67 residing in the periplasm. Coordinate induction by oligoxylosides ofxyn10,agu67,cirA, thetonBreceptor, and other genes within these three operons indicates that they constitute a regulon that is responsive to the oligoxylosides generated by the action ofXcc306 GH10 xylanases on MeGXn. The combined expression of genes in this regulon may allow scavenging of oligoxylosides derived from cell wall deconstruction, thereby contributing to the tissue colonization and/or survival ofXcc306 and, ultimately, to plant disease.

scholarly journals Effects of Melatonin Treatment of Postharvest Pear Fruit on Aromatic Volatile Biosynthesis

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4233 ◽  
Author(s):  
Jianlong Liu ◽  
Hanting Liu ◽  
Ting Wu ◽  
Rui Zhai ◽  
Chengquan Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Consumer Satisfaction ◽  
Storage Period ◽  
Hydroperoxide Lyase ◽  
Pear Fruit ◽  
Expression Of Genes ◽  
Pyrus Communis L ◽  
Genes Encoding ◽  
Alcohol Acyltransferase

Aroma affects the sensory quality of fruit and, consequently, consumer satisfaction. Melatonin (MT) is a plant growth regulator used to delay senescence in postharvest fruit during storage; however, its effect on aroma of pear fruit remains unclear. In this study, we assessed the effects of 0.1 mmol L−1 MT on volatiles and associated gene expression in the fruit of pear cultivars ‘Korla’ (Pyrus brestschneideri Rehd) and ‘Abbé Fetel’ (Pyrus communis L.). MT mainly affected the production of C6 aromatic substances in the two varieties. In ‘Korla’, MT inhibited expression of PbHPL, and reduced hydroperoxide lyase (HPL) activity and content of hexanal and (E)-hex-2-enal. In contrast, MT inhibited activity of lipoxygenase (LOX), reduced expression of PbLOX1 and PbLOX2, promoted PbAAT gene expression, increased alcohol acyltransferase (AAT) activity, and increased propyl acetate, and hexyl acetate content in ‘Abbé Fetel’ that similarly led to the reduction in content of hexanal and (E)-hex-2-enal. Content of esters in ‘Abbé Fetel’ pear increased with increasing postharvest storage period. Although mechanisms differed between the two varieties, effects on aroma volatiles mediated by MT were driven by expression of genes encoding LOX, HPL, and AAT enzymes.

scholarly journals Effect of Ethylene on Cell Wall and Lipid Metabolism during Alleviation of Postharvest Chilling Injury in Peach

Cells ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 1612 ◽  
Author(s):  
Yongchao Zhu ◽  
Ke Wang ◽  
Chunxia Wu ◽  
Yun Zhao ◽  
Xueren Yin ◽  
...  
Keyword(s):  
Cell Wall ◽  
Lipid Metabolism ◽  
Chilling Injury ◽  
Response Factors ◽  
Ethylene Treatment ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Endogenous Ethylene ◽  
Highly Correlated ◽  
Exogenous Ethylene

Peach is prone to postharvest chilling injury (CI). Here it was found that exogenous ethylene alleviated CI, accompanied by an increased endogenous ethylene production. Ethylene treatment resulted in a moderately more rapid flesh softening as a result of stronger expression of genes encoding expansin and cell wall hydrolases, especially xylosidase and galactosidase. Ethylene treatment alleviated internal browning, accompanied by changes in expression of polyphenol oxidase, peroxidase and lipoxygenases. An enhanced content of phospholipids and glycerolipids and a reduced content of ceramide were observed in ethylene-treated fruit, and these were associated with up-regulation of lipid phosphate phosphatase, fatty acid alpha-hydroxylase, and golgi-localized nucleotide sugar transporter, as well as down-regulation of aminoalcohol phosphotransferases. Expression of two ethylene response factors (ERFs), ESE3 and ABR1, was highly correlated with that of genes involved in cell wall metabolism and lipid metabolism, respectively. Furthermore, the expression of these two ERFs was strongly regulated by ethylene treatment and the temperature changes during transfer of fruit into or out of cold storage. It is proposed that ERFs fulfill roles as crucial integrators between cell wall modifications and lipid metabolism involved in CI processes ameliorated by exogenous ethylene.

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