scholarly journals Characterization of two putative ethylene receptor genes expressed during peach fruit development and abscission

2002 ◽  
Vol 53 (379) ◽  
pp. 2333-2339 ◽  
Author(s):  
A. Rasori ◽  
B. Ruperti ◽  
C. Bonghi ◽  
P. Tonutti ◽  
A. Ramina
Keyword(s):  
Fruit Development ◽  
Ethylene Receptor ◽  
Peach Fruit

scholarly journals Seasonal Expression of a 700–800-Base Pair Transcript in Bark Tissues of Peach (Prunus persica)

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 514B-514
Author(s):  
Michael Wisniewski ◽  
Tim Artlip ◽  
Carole Bassett ◽  
Ann Callahan
Keyword(s):  
Fruit Development ◽  
Seasonal Changes ◽  
Woody Plants ◽  
Cold Hardiness ◽  
Prunus Persica ◽  
Storage Proteins ◽  
Proteinase Inhibitors ◽  
Peach Fruit ◽  
Genes Encoding

Cold acclimation in temperate, woody plants involves distinct changes in gene activity and protein expression. We have been identifying proteins and genes that are associated with seasonal changes in cold hardiness. Seasonal changes in a 60-kDa dehydrin and its corresponding transcript have been identified, as well as seasonal changes in 16- and 19-kDa storage proteins. Further screening of a cDNA library, constructed from cold-acclimated bark tissues collected in December, identified a 700–800-bp clone that was seasonally expressed in Northern blots. The transcript began to accumulate in October, reached a peak in November–December, and then began to decline. By April, the transcript was no longer present in bark tissues. The transcript size indicates that this gene my be related to either the 16- or 19-kDa storage proteins previously identified; however, an amino acid sequence of the protein for comparison has not yet been obtained. Interestingly, the transcript is also expressed during the early stages of peach fruit development. A similar pattern between seasonal expression and fruit development has been observed for a peach dehydrin transcript. Analysis of a partial sequence of the clone has indicated a similarity to genes encoding proteinase inhibitors and thionins (a class of biocidal proteins). More definitive characterization of the gene and identification of its corresponding protein are in progress.

scholarly journals Isolation and Initial Characterization of cDNAs for mRNAs Regulated during Peach Fruit Development

1993 ◽  
Vol 118 (4) ◽  
pp. 531-537 ◽  
Author(s):  
Ann M. Callahan ◽  
Peter H. Morgens ◽  
Reuben A. Cohen
Keyword(s):  
Fruit Development ◽  
Prunus Persica ◽  
Cdna Libraries ◽  
Cdna Clones ◽  
Ripe Fruit ◽  
Peach Fruit ◽  
Initial Characterization ◽  
Initial Source ◽  
Softening Stage

Peach [Prunus persica (L.) Batsch] cDNA libraries have been constructed from RNA isolated from immature (30 days after bloom) and ripe fruit. cDNA clones of interest have been identified by differential hybridization among the cDNAs of various peach cultivars or from several stages in fruit development. In addition, several clones were isolated by low stringency hybridizations with oligonucleotides derived from a tomato polygalacturonase cDNA sequence and a cucumber peroxidase amino acid sequence. The pattern of accumulation of the corresponding mRNAs during fruit development was examined by RNA gel-blot analyses in the commercial cultivar Suncrest. Three cDNA clones, pch201, pch307, and pch313, were related to mRNAs that accumulate during the softening stage of fruit development. cDNA clones pchl03, pch205, and pch306 were related to an mRNAs that increase in abundance throughout development, with maximum levels in ripe fruit. cDNA clones pch104 and pch202 were related to mRNAs that exhibit maximum abundance in midfruit development, and clone pch108 was related to mRNA that decreases as the fruit matures. Southern analyses indicated that seven of the cDNAs are represented by only a few genes, while pch104 detects a repetitive family, and pch307 detects a small family of genes. These clones will provide the initial source of genes to manipulate and affect fruit development.

Molecular characterization of an ethylene receptor gene (CcETR1) in coffee trees, its relationship with fruit development and caffeine content

2007 ◽  
Vol 277 (6) ◽  
pp. 701-712 ◽  
Author(s):  
José Bustamante-Porras ◽  
Claudine Campa ◽  
Valérie Poncet ◽  
Michel Noirot ◽  
Thierry Leroy ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Fruit Development ◽  
Receptor Gene ◽  
Ethylene Receptor ◽  
Caffeine Content

scholarly journals Genome-wide identification and characterization of COMT gene family during the development of blueberry fruit

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yushan Liu ◽  
Yizhou Wang ◽  
Jiabo Pei ◽  
Yadong Li ◽  
Haiyue Sun
Keyword(s):  
Gene Family ◽  
Fruit Development ◽  
Lignin Content ◽  
Phylogenetic Analyses ◽  
Expression Patterns ◽  
Vaccinium Corymbosum ◽  
Land Plant ◽  
Phylogenetic Structure ◽  
Genome Wide

Abstract Background Caffeic acid O-methyltransferases (COMTs) play an important role in the diversification of natural products, especially in the phenylalanine metabolic pathway of plant. The content of COMT genes in blueberry and relationship between their expression patterns and the lignin content during fruit development have not clearly investigated by now. Results Ninety-two VcCOMTs were identified in Vaccinium corymbosum. According to phylogenetic analyses, the 92 VcCOMTs were divided into 2 groups. The gene structure and conserved motifs within groups were similar which supported the reliability of the phylogenetic structure groupings. Dispersed duplication (DSD) and whole-genome duplication (WGD) were determined to be the major forces in VcCOMTs evolution. The results showed that the results of qRT-PCR and lignin content for 22 VcCOMTs, VcCOMT40 and VcCOMT92 were related to lignin content at different stages of fruit development of blueberry. Conclusion We identified COMT gene family in blueberry, and performed comparative analyses of the phylogenetic relationships in the 15 species of land plant, and gene duplication patterns of COMT genes in 5 of the 15 species. We found 2 VcCOMTs were highly expressed and their relative contents were similar to the variation trend of lignin content during the development of blueberry fruit. These results provide a clue for further study on the roles of VcCOMTs in the development of blueberry fruit and could promisingly be foundations for breeding blueberry clutivals with higher fruit firmness and longer shelf life.

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