scholarly journals Room temperature microspectrofluorimetry as a useful tool for studying the assembly of the PSII chlorophyll-protein complexes in single living cells of etiolated Euglena gracilis Klebs during the greening process

2002 ◽  
Vol 53 (375) ◽  
pp. 1753-1763 ◽  
Author(s):  
S. Pancaldi
Keyword(s):  
Euglena Gracilis ◽  
Room Temperature ◽  
Protein Complexes ◽  
Living Cells ◽  
Chlorophyll Protein Complexes ◽  
Chlorophyll Protein ◽  
Single Living Cells ◽  
Single Living

Revised assignment of room-temperature chlorophyll fluorescence emission bands in single living cells of Chlamydomonas reinhardtii

2011 ◽  
Vol 43 (2) ◽  
pp. 163-173 ◽  
Author(s):  
Lorenzo Ferroni ◽  
Costanza Baldisserotto ◽  
Martina Giovanardi ◽  
Laura Pantaleoni ◽  
Tomas Morosinotto ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Chlamydomonas Reinhardtii ◽  
Room Temperature ◽  
Fluorescence Emission ◽  
Living Cells ◽  
Single Living Cells ◽  
Single Living

Application of FRAP and digitized fluorescence microscopy to problems in cell membrane dynamics

1988 ◽  
Vol 46 ◽  
pp. 118-119
Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Extended Period ◽  
Dynamic Structure ◽  
Living Cells ◽  
Membrane Dynamics ◽  
Molecular Cell Biology ◽  
Image Averaging ◽  
Single Living Cells ◽  
Single Living

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.

Photoinhibition in vivo and in vitro Involves Weakly Coupled Chlorophyll–Protein Complexes‡¶

2002 ◽  
Vol 75 (6) ◽  
pp. 613 ◽  
Author(s):  
Stefano Santabarbara ◽  
Ilaria Cazzalini ◽  
Andrea Rivadossi ◽  
Flavio M. Garlaschi ◽  
Giuseppe Zucchelli ◽  
...  
Keyword(s):  
Protein Complexes ◽  
Weakly Coupled ◽  
Chlorophyll Protein Complexes ◽  
Chlorophyll Protein
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