BES1/BZR1 Homolog 3 cooperates with E3 ligase AtRZF1 to regulate osmotic stress and brassinosteroid responses in Arabidopsis

2020 ◽  
Author(s):  
Tinh Van Nguyen ◽  
Cho-Rong Park ◽  
Kyeong-Hwan Lee ◽  
Sungbeom Lee ◽  
Cheol Soo Kim
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Osmotic Stress ◽  
Redox Balance ◽  
Molecular Networks ◽  
Dependent Manner ◽  
Abiotic Stress Response ◽  
Gene Encoding ◽  
Species Analysis ◽  
Osmotic Stress Response

Abstract Proline (Pro) metabolism plays important roles in protein synthesis, redox balance, and abiotic stress response. However, it is not known if cross-talk occurs between proline and brassinosteroid (BR) signaling pathways. Here, an Arabidopsis intergenic enhancer double mutant, namely proline content alterative 41 (pca41), was generated by inserting a T-DNA tag in the Arabidopsis thaliana ring zinc finger 1 (atrzf1 ) mutant background. pca41 had a T-DNA inserted at the site of the gene encoding BES1/BZR1 Homolog 3 (BEH3). pca41 has a drought-insensitive phenotype that is stronger than atrzf1 under osmotic stress, including high Pro accumulation and decreased amounts of reactive oxygen species. Analysis of physiological, genetic, and molecular networks revealed that negative regulation of BEH3 during abiotic stress was linked to the BR signaling pathway. Our data also suggest that AtRZF1, an E3 ubiquitin ligase, might control osmotic stress, abscisic acid, and BR responses in a BEH3-dependent manner. Under darkness, pca41 displays a long hypocotyl phenotype, which is similar to atrzf1 and beh3, suggesting that BEH3 acts in the same pathway as AtRZF1. Overexpression of BEH3 results in an osmotic stress-sensitive phenotype, which is reversed by exogenous BR application. Taken together, our results indicate that AtRZF1 and BEH3 may play important roles in the osmotic stress response via ubiquitination and BR signaling.

scholarly journals Genome-Wide Identification and Expression Analyses of the Chitinases under Cold and Osmotic stress in Ammopiptanthus nanus

Genes ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 472 ◽  
Author(s):  
Cao ◽  
Wang ◽  
Li ◽  
Shi ◽  
Gao ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Low Temperature ◽  
Osmotic Stress ◽  
Fungal Pathogens ◽  
Glycosyl Hydrolase ◽  
Class Iii ◽  
Abiotic Stress Response ◽  
Ammopiptanthus Nanus ◽  
Genome Wide

Chitinase is a kind of hydrolase with chitin as a substrate and is proposed to play an essential role in plant defense system by functioning against fungal pathogens through degrading chitin. Recent studies indicated chitinase is also involved in abiotic stress response in plants, helping plants to survive in stressful environments. A. nanus, a rare evergreen broad-leaved shrub distrusted in deserts in Central Asia, exhibits a high level of tolerance to drought and low temperature stresses. To identify the chitinase gene involved in drought and low temperature responses in A. nanus, we performed genome-wide identification, classification, sequence alignment, and spatio-temporal gene expression analysis of the chitinases in A. nanus under osmotic and low temperature stress. A total of 32 chitinase genes belonging to glycosyl hydrolase 18 (GH18) and GH19 families were identified from A. nanus. Class III chitinases appear to be amplified quantitatively in A. nanus, and their genes carry less introns, indicating their involvement in stress response in A. nanus. The expression level of the majority of chitinases varied in leaves, stems, and roots, and regulated under environmental stress. Some chitinases, such as EVM0022783, EVM0020238, and EVM0003645, are strongly induced by low temperature and osmotic stress, and the MYC/ICE1 (inducer of CBF expression 1) binding sites in promoter regions may mediate the induction of these chitinases under stress. These chitinases might play key roles in the tolerance to these abiotic stress in A. nanus and have potential for biotechnological applications. This study provided important data for understanding the biological functions of chitinases in A. nanus.

scholarly journals NDR1 and the Arabidopsis Plasma Membrane ATPase AHA5 are Required for Processes that Converge on Drought Tolerance and Immunity

2021 ◽  
Author(s):  
Yi-Ju Lu ◽  
Huan Chen ◽  
Alex Corrion ◽  
Pai Li ◽  
Ilker Buyuk ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Abiotic Stress ◽  
Stress Response ◽  
Pseudomonas Syringae ◽  
Stress Responses ◽  
Cell Biology ◽  
Dependent Manner ◽  
Abiotic Stress Response ◽  
Biotic And Abiotic Stress ◽  
Plasma Membrane Atpase

NON-RACE-SPECIFIC DISEASE RISISTANCE1 (NDR1) is a key component of plant immune signaling, required for defense against the bacterial pathogen Pseudomonas syringae. Plant stress responses have overlapping molecular, physiological, and cell biology signatures, and given the central role of NDR1 during biotic stress perception and signaling, we hypothesized that NDR1 also functions in abiotic stress responses, including in a role that mediates signaling at the plasma membrane (PM) - cell wall (CW) continuum. Here, we demonstrate that NDR1 is required for the induction of drought stress responses in plants, a role that couples stress signaling in an abscisic acid-dependent manner. We show that NDR1 physically associates with the PM-localized H+-ATPases AHA1, AHA2 , and AHA5 and is required for proper regulation of H+-ATPase activity and stomatal guard cell dynamics, providing a mechanistic function of NDR1 during drought responses. In the current study, we demonstrate that NDR1 functions in signaling processes associated with both biotic and abiotic stress response pathways, a function we hypothesize represents NDR1's role in the maintenance of cellular homeostasis during stress. We propose a role for NDR1 as a core transducer of signaling between cell membrane processes and intercellular stress response activation.

scholarly journals NMR and LC-MS-Based Metabolomics to Study Osmotic Stress in Lignan-Deficient Flax

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 767
Author(s):  
Kamar Hamade ◽  
Ophélie Fliniaux ◽  
Jean-Xavier Fontaine ◽  
Roland Molinié ◽  
Elvis Otogo Nnang ◽  
...  
Keyword(s):  
Stress Response ◽  
Osmotic Stress ◽  
Biosynthetic Pathway ◽  
Potential Role ◽  
Plant Secondary Metabolites ◽  
Wild Type ◽  
Osmotic Stress Response ◽  
Transgenic Flax ◽  
Insight Into

Lignans, phenolic plant secondary metabolites, are derived from the phenylpropanoid biosynthetic pathway. Although, being investigated for their health benefits in terms of antioxidant, antitumor, anti-inflammatory and antiviral properties, the role of these molecules in plants remains incompletely elucidated; a potential role in stress response mechanisms has been, however, proposed. In this study, a non-targeted metabolomic analysis of the roots, stems, and leaves of wild-type and PLR1-RNAi transgenic flax, devoid of (+) secoisolariciresinol diglucoside ((+) SDG)—the main flaxseed lignan, was performed using 1H-NMR and LC-MS, in order to obtain further insight into the involvement of lignan in the response of plant to osmotic stress. Results showed that wild-type and lignan-deficient flax plants have different metabolic responses after being exposed to osmotic stress conditions, but they both showed the capacity to induce an adaptive response to osmotic stress. These findings suggest the indirect involvement of lignans in osmotic stress response.

scholarly journals The Raf-like kinase ILK1 and the high affinity K+ transporter HAK5 are required for Innate Immunity and Abiotic Stress Response

2016 ◽  
pp. pp.00035.2016 ◽  
Author(s):  
Elizabeth Kalinda Brauer ◽  
Nagib Ahsan ◽  
Renee Dale ◽  
Naohiro Kato ◽  
Alison E Coluccio ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Abiotic Stress ◽  
Stress Response ◽  
Abiotic Stress Response ◽  
High Affinity

scholarly journals Functional genomics and proteomics of the cellular osmotic stress response in `non-model' organisms

2007 ◽  
Vol 210 (9) ◽  
pp. 1593-1601 ◽  
Author(s):  
D. Kultz ◽  
D. Fiol ◽  
N. Valkova ◽  
S. Gomez-Jimenez ◽  
S. Y. Chan ◽  
...  
Keyword(s):  
Stress Response ◽  
Osmotic Stress ◽  
Functional Genomics ◽  
Model Organisms ◽  
Genomics And Proteomics ◽  
Osmotic Stress Response
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