scholarly journals Feeling the heat: developmental and molecular responses of wheat and barley to high ambient temperatures

2020 ◽  
Vol 71 (19) ◽  
pp. 5740-5751 ◽  
Author(s):  
Catherine N Jacott ◽  
Scott A Boden
Keyword(s):  
Developmental Stages ◽  
Genetic Regulation ◽  
Reproductive Traits ◽  
High Temperature Stress ◽  
Future Research ◽  
Inflorescence Development ◽  
Molecular Responses ◽  
Global Food Security ◽  
Ambient Temperatures ◽  
Increasing Demand

Abstract The increasing demand for global food security in the face of a warming climate is leading researchers to investigate the physiological and molecular responses of cereals to rising ambient temperatures. Wheat and barley are temperate cereals whose yields are adversely affected by high ambient temperatures, with each 1 °C increase above optimum temperatures reducing productivity by 5–6%. Reproductive development is vulnerable to high-temperature stress, which reduces yields by decreasing grain number and/or size and weight. In recent years, analysis of early inflorescence development and genetic pathways that control the vegetative to floral transition have elucidated molecular processes that respond to rising temperatures, including those involved in the vernalization- and photoperiod-dependent control of flowering. In comparison, our understanding of genes that underpin thermal responses during later developmental stages remains poor, thus highlighting a key area for future research. This review outlines the responses of developmental genes to warmer conditions and summarizes our knowledge of the reproductive traits of wheat and barley influenced by high temperatures. We explore ways in which recent advances in wheat and barley research capabilities could help identify genes that underpin responses to rising temperatures, and how improved knowledge of the genetic regulation of reproduction and plant architecture could be used to develop thermally resilient cultivars.

scholarly journals Comparative Transcriptomic Analysis of Riptortus pedestris (Hemiptera: Alydidae) to Characterize Wing Formation across All Developmental Stages

Insects ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 226
Author(s):  
Siying Fu ◽  
Yujie Duan ◽  
Siqi Wang ◽  
Yipeng Ren ◽  
Wenjun Bu
Keyword(s):  
Molecular Mechanisms ◽  
Developmental Stages ◽  
Average Length ◽  
Transcriptome Assembly ◽  
Expression Profiles ◽  
Economic Losses ◽  
Future Research ◽  
Economic Damage ◽  
Wing Formation ◽  
Riptortus Pedestris

Riptortus pedestris (Hemiptera: Alydidae) is a major agricultural pest in East Asia that causes considerable economic losses to the soybean crop each year. However, the molecular mechanisms governing the growth and development of R. pedestris have not been fully elucidated. In this study, the Illumina HiSeq6000 platform was employed to perform de novo transcriptome assembly and determine the gene expression profiles of this species across all developmental stages, including eggs, first-, second-, third-, fourth-, and fifth-instar nymphs, and adults. In this study, a total of 60,058 unigenes were assembled from numerous raw reads, exhibiting an N50 length of 2126 bp and an average length of 1199 bp, and the unigenes were annotated and classified with various databases, such as the Kyoto Encyclopedia of Genes and Genomes (KEGG), Clusters of Orthologous Groups (COG), and Gene Ontology (GO). Furthermore, various numbers of differentially expressed genes (DEGs) were calculated through pairwise comparisons of all life stages, and some of these DEGs were associated with immunity, metabolism, and development by GO and KEGG enrichment. In addition, 35,158 simple sequence repeats (SSRs) and 715,604 potential single nucleotide polymorphisms (SNPs) were identified from the seven transcriptome libraries of R. pedestris. Finally, we identified and summarized ten wing formation-related signaling pathways, and the molecular properties and expression levels of five wing development-related genes were analyzed using quantitative real-time PCR for all developmental stages of R. pedestris. Taken together, the results of this study may establish a foundation for future research investigating developmental processes and wing formation in hemimetabolous insects and may provide valuable data for pest control efforts attempting to reduce the economic damage caused by this pest.

scholarly journals Comparison of the Transcriptomic and Epigenetic Profiles of Gonadal Primordial Germ Cells of White Leghorn and Green-Legged Partridgelike Chicken Embryos

Genes ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 1090
Author(s):  
Aleksandra Dunislawska ◽  
Maria Siwek ◽  
Katarzyna Stadnicka ◽  
Marek Bednarczyk
Keyword(s):  
Embryonic Development ◽  
Germ Cells ◽  
Developmental Stages ◽  
Primordial Germ Cells ◽  
Genetic Material ◽  
Reproductive Traits ◽  
Germline Stem Cells ◽  
White Leghorn ◽  
Methylation Analysis ◽  
Global Methylation

The Green-legged Partridgelike fowl is a native, dual-purpose Polish chicken. The White Leghorn has been intensively selected for several decades to mainly improve reproductive traits. Primordial germ cells (PGCs) represent the germline stem cells in chickens and are the only cells that can transfer the information stored in the genetic material from generation to generation. The aim of the study was to carry out a transcriptomic and an epigenetic comparison of the White Leghorn and Green-legged Partridgelike gonadal PGCs (gPGCs) at three developmental stages: days 4.5, 8, and 12 of the embryonic development. RNA and DNA were isolated from collected gPGCs. The RNA was further subjected to microarray analysis. An epigenetic analysis was performed based on the global methylation analysis and qMSP method for the particular silenced genes demonstrated in transcriptomic analysis. Statistically significant differences between the gPGCs from both breeds were detected on the day 8 of embryonic development. Global methylation analysis showed significant changes at the methylation level in the White Leghorn gPGCs on day 8 of embryonic development. The results suggest faster development of Green-legged Partridgelike embryos as compared to White Leghorn embryos. Changes in the levels of gene expression during embryonic development are determined by genetic and environmental factors, and this variability is influenced by breed and gender.

scholarly journals Heat unit accumulation and inflorescence and fruit development in ‘Ubá’ mango trees grown in Visconde do Rio Branco-MG

2018 ◽  
Vol 40 (2) ◽  
Author(s):  
Lorena Moreira Carvalho Lemos ◽  
Luiz Carlos Chamhum Salomão ◽  
Dalmo Lopes de Siqueira ◽  
Olinto Liparini Pereira ◽  
Paulo Roberto Cecon
Keyword(s):  
Fruit Development ◽  
Fruit Set ◽  
Developmental Stages ◽  
Mangifera Indica ◽  
Developmental Cycle ◽  
Flower Bud ◽  
Ambient Temperatures ◽  
Heat Units ◽  
Mango Tree ◽  
Apical Bud

Abstract There are little information in the scientific literature on flowering and fruiting of ‘Ubá’ mango trees. These information enables to know the proportion of hermaphrodite flowers in inflorescence, fruit set percentage and developmental stages of the fruit. In this study evaluations on inflorescence and fruit development of the ‘Ubá’ mango tree (Mangifera indica L.) were carried out, as well as the determination of the required number of heat units for full fruit development. Thirty branches whose terminal buds were swollen were selected from five mango trees. With the aid of a camera and a caliper, the panicle and fruit development were evaluated weekly until full fruit development. A digital thermometer was used to record ambient temperatures during fruit development in order to estimate the number of heat units required for complete development of the fruits. Male and hermaphrodite flowers of the panicles were also identified and counted. The developmental cycle of ‘Ubá’ mango from the beginning of apical bud swelling to commercial harvest of the fruit lasted 168 days in 2011 and 154 days in 2012. The number of hermaphrodite flowers and the percentage of fruit set in the inflorescence in 2011 were 32.3 and 0.066%, respectively; and 122.1 and 0.099% in 2012, respectively. There was accumulation of 3,173 heat units from flower bud swelling to full development of the ‘Ubá’ mangoes.

Biochemical and molecular responses of herbaceous peony to high temperature stress

2016 ◽  
Vol 96 (3) ◽  
pp. 474-484 ◽  
Author(s):  
Yan-Qing Wu ◽  
Da-Qiu Zhao ◽  
Chen-Xia Han ◽  
Jun Tao
Keyword(s):  
Antioxidant Enzymes ◽  
High Temperature ◽  
Temperature Stress ◽  
Theoretical Basis ◽  
High Temperature Stress ◽  
Cellular Structures ◽  
Molecular Responses ◽  
Chl A ◽  
Antioxidant Enzymes Activities ◽  
Heat Tolerant

To clarify the theoretical basis of the differences in high temperature stress tolerance among herbaceous peony (Paeonia lactiflora Pall.), we investigated the heat injury index of twelve P. lactiflora cultivars. Of these, heat-tolerant ‘Zifengyu’ and moderately heat-tolerant ‘Hongyanzhenghui’ were selected to study the biochemical and molecular responses to high temperature stress. ‘Zifengyu’ had reduced malondialdehyde (MDA) content, increased soluble sugar, chlorophyll (Chl) a, Chl b, Chl a + b. and carotenoid contents, as well as elevated antioxidant enzymes activities, photosynthetic rate (Pn), transpiration rate (Tr) and relatively intact cellular structures compared with ‘Hongyanzhenghui’, especially when the temperature was the highest. Additionally, we isolated partial cDNAs of two heat shock protein genes (HSP60 and HSP90) from P. lactiflora, which were 880-bp and 1077-bp nucleotides in length, respectively. The expression levels of PlHSP60, PlHSP70 and PlHSP90 were lower in ‘Zifengyu’ than in ‘Hongyanzhenghui’ for the first three of four developmental stages examined. These results indicated that heat-tolerant P. lactiflora cultivar could effectively scavenge reactive oxygen species (ROS), protect cellular structures, reduce thermal damage and delay the death of plants by enhancing antioxidant enzymes activities and HSP expression under high temperature stress. These findings provide a theoretical basis for breeding heat-tolerant P. lactiflora cultivars.

scholarly journals Advances in Computational Intelligence of Polymer Composite Materials: Machine Learning Assisted Modeling, Analysis and Design

2021 ◽  
Author(s):  
Aanchna Sharma ◽  
Tanmoy Mukhopadhyay ◽  
Sanjay Mavinkere Rangappa ◽  
Suchart Siengchin ◽  
Vinod Kushvaha
Keyword(s):  
Machine Learning ◽  
Polymer Composites ◽  
Relevant Literature ◽  
Future Research ◽  
Extensive Literature ◽  
Feature Identification ◽  
Predictive Tool ◽  
Analysis And Design ◽  
Increasing Demand ◽  
System Properties

Abstract The superior multi-functional properties of polymer composites have made them an ideal choice for aerospace, automobile, marine, civil, and many other technologically demanding industries. The increasing demand of these composites calls for an extensive investigation of their physical, chemical and mechanical behavior under different exposure conditions. Machine learning (ML) has been recognized as a powerful predictive tool for data-driven multi-physical modeling, leading to unprecedented insights and exploration of the system properties beyond the capability of traditional computational and experimental analyses. Here we aim to abridge the findings of the large volume of relevant literature and highlight the broad spectrum potential of ML in applications like prediction, optimization, feature identification, uncertainty quantification, reliability and sensitivity analysis along with the framework of different ML algorithms concerning polymer composites. Challenges like the curse of dimensionality, overfitting, noise and mixed variable problems are discussed, including the latest advancements in ML that have the potential to be integrated in the field of polymer composites. Based on the extensive literature survey, a few recommendations on the exploitation of various ML algorithms for addressing different critical problems concerning polymer composites are provided along with insightful perspectives on the potential directions of future research.

230 Microalgae as an Alternative Feed Protein: Opportunities and Challenges

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 119-120
Author(s):  
Xingen Lei
Keyword(s):  
Food Security ◽  
Production Performance ◽  
Biofuel Production ◽  
Future Research ◽  
Production Volume ◽  
Food Protein ◽  
Microalgal Biomass ◽  
Global Food Security ◽  
Feed Protein ◽  
Global Food

Abstract A major mission of the animal industry is to help protect global food security with little or no impacts on climate and environment. In fact, the global food security depends on a sustainable protein production and supply. Currently, there is a direct competition between feed and food protein need. As animal feeds use 70–80% of the produced soybean, alternative proteins are required to sustain the industry. This presentation will report research findings from three large consortium projects in exploring the dual application of microalgae as a new generation of feedstock for biofuel production and high-quality feed protein supply. A comprehensive review will be provided on effects of supplemental full- or de-fatted microalgal biomass in diets for broiler chickens, laying hens, pigs, and fish on their production performance, nutrient metabolism and molecular responses, and health values of their products. Discussions will be given on practical concerns over the production cost related to soybean meal, the production volume/scale for a constant supply, and the biosafety and acceptance as a regular ingredient. Future research will be proposed to synchronize the nutritional, health, and sustainability potential of microalgae as a viable feed protein source.

scholarly journals Tissue specific regulation of transcription in endometrium and association with disease

2020 ◽  
Vol 35 (2) ◽  
pp. 377-393 ◽  
Author(s):  
Sally Mortlock ◽  
Raden I Kendarsari ◽  
Jenny N Fung ◽  
Greg Gibson ◽  
Fei Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Association Study ◽  
Genetic Variants ◽  
Genetic Regulation ◽  
Regulation Of Gene Expression ◽  
Enrichment Analysis ◽  
Reproductive Traits ◽  
Genetic Effects ◽  
Genotype Data ◽  
Tissue Specific

Abstract STUDY QUESTION Are genetic effects on endometrial gene expression tissue specific and/or associated with reproductive traits and diseases? SUMMARY ANSWER Analyses of RNA-sequence data and individual genotype data from the endometrium identified novel and disease associated, genetic mechanisms regulating gene expression in the endometrium and showed evidence that these mechanisms are shared across biologically similar tissues. WHAT IS KNOWN ALREADY The endometrium is a complex tissue vital for female reproduction and is a hypothesized source of cells initiating endometriosis. Understanding genetic regulation specific to, and shared between, tissue types can aid the identification of genes involved in complex genetic diseases. STUDY DESIGN, SIZE, DURATION RNA-sequence and genotype data from 206 individuals was analysed and results were compared with large publicly available datasets. PARTICIPANTS/MATERIALS, SETTING, METHODS RNA-sequencing and genotype data from 206 endometrial samples was used to identify the influence of genetic variants on gene expression, via expression quantitative trait loci (eQTL) analysis and to compare these endometrial eQTLs with those in other tissues. To investigate the association between endometrial gene expression regulation and reproductive traits and diseases, we conducted a tissue enrichment analysis, transcriptome-wide association study (TWAS) and summary data-based Mendelian randomisation (SMR) analyses. Transcriptomic data was used to test differential gene expression between women with and without endometriosis. MAIN RESULTS AND THE ROLE OF CHANCE A tissue enrichment analysis with endometriosis genome-wide association study summary statistics showed that genes surrounding endometriosis risk loci were significantly enriched in reproductive tissues. A total of 444 sentinel cis-eQTLs (P < 2.57 × 10−9) and 30 trans-eQTLs (P < 4.65 × 10−13) were detected, including 327 novel cis-eQTLs in endometrium. A large proportion (85%) of endometrial eQTLs are present in other tissues. Genetic effects on endometrial gene expression were highly correlated with the genetic effects on reproductive (e.g. uterus, ovary) and digestive tissues (e.g. salivary gland, stomach), supporting a shared genetic regulation of gene expression in biologically similar tissues. The TWAS analysis indicated that gene expression at 39 loci is associated with endometriosis, including five known endometriosis risk loci. SMR analyses identified potential target genes pleiotropically or causally associated with reproductive traits and diseases including endometriosis. However, without taking account of genetic variants, a direct comparison between women with and without endometriosis showed no significant difference in endometrial gene expression. LARGE SCALE DATA The eQTL dataset generated in this study is available at http://reproductivegenomics.com.au/shiny/endo_eqtl_rna/. Additional datasets supporting the conclusions of this article are included within the article and the supplementary information files, or are available on reasonable request. LIMITATIONS, REASONS FOR CAUTION Data are derived from fresh tissue samples and expression levels are an average of expression from different cell types within the endometrium. Subtle cell-specifc expression changes may not be detected and differences in cell composition between samples and across the menstrual cycle will contribute to sample variability. Power to detect tissue specific eQTLs and differences between women with and without endometriosis was limited by the sample size in this study. The statistical approaches used in this study identify the likely gene targets for specific genetic risk factors, but not the functional mechanism by which changes in gene expression may influence disease risk. WIDER IMPLICATIONS OF THE FINDINGS Our results identify novel genetic variants that regulate gene expression in endometrium and the majority of these are shared across tissues. This allows analysis with large publicly available datasets to identify targets for female reproductive traits and diseases. Much larger studies will be required to identify genetic regulation of gene expression that will be specific to endometrium. STUDY FUNDING/COMPETING INTEREST(S) This work was supported by the National Health and Medical Research Council (NHMRC) under project grants GNT1026033, GNT1049472, GNT1046880, GNT1050208, GNT1105321, GNT1083405 and GNT1107258. G.W.M is supported by a NHMRC Fellowship (GNT1078399). J.Y is supported by an ARC Fellowship (FT180100186). There are no competing interests.

scholarly journals Enantiomers of Carbohydrates and Their Role in Ecosystem Interactions: A Review

Symmetry ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 470 ◽  
Author(s):  
Lea Lojkova ◽  
Valerie Vranová ◽  
Pavel Formánek ◽  
Ida Drápelová ◽  
Martin Brtnicky ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Plant Nutrition ◽  
Current Knowledge ◽  
Genetic Regulation ◽  
Future Research ◽  
Knowledge Gaps ◽  
Research Knowledge ◽  
Critical Knowledge ◽  
Ecosystem Interactions ◽  
Plant Compounds

D- and most L-enantiomers of carbohydrates and carbohydrate-containing compounds occur naturally in plants and other organisms. These enantiomers play many important roles in plants including building up biomass, defense against pathogens, herbivory, abiotic stress, and plant nutrition. Carbohydrate enantiomers are also precursors of many plant compounds that significantly contribute to plant aroma. Microorganisms, insects, and other animals utilize both types of carbohydrate enantiomers, but their biomass and excrements are dominated by D-enantiomers. The aim of this work was to review the current knowledge about carbohydrate enantiomers in ecosystems with respect to both their metabolism in plants and occurrence in soils, and to identify critical knowledge gaps and directions for future research. Knowledge about the significance of D- versus L-enantiomers of carbohydrates in soils is rare. Determining the mechanism of genetic regulation of D- and L-carbohydrate metabolism in plants with respect to pathogen and pest control and ecosystem interactions represent the knowledge gaps and a direction for future research.

scholarly journals A-44 ImPACT of Amnesia Related Concussions Across Developmental Stages

2020 ◽  
Vol 35 (5) ◽  
pp. 640-640
Author(s):  
K Unjia ◽  
R Bennett ◽  
L Lashley
Keyword(s):  
Retrograde Amnesia ◽  
Sports Medicine ◽  
Developmental Stages ◽  
Future Research ◽  
Age Group ◽  
Adolescent Athletes ◽  
Significant Difference ◽  
The Impact ◽  
The Relationship ◽  
One Way Anova

Abstract Objective This study aimed to examine the relationship between developmental stages and concussions that resulted in amnesia as measured by ImPACT. Method Participants were selected from an archival de-identified sports medicine ImPACT database. The sample (N = 4,200) was primarily male (62.2%) student athletes with ages ranging from 10 to 25 years. Participants were divided into three groups: Young athletes (n = 1,400), Adolescent (n = 1,400), and Adult (n = 1,400). A One-Way ANOVA was conducted to determine the relationship between age group and prevalence of concussions resulting in anterograde or retrograde amnesia. Results The One-Way ANOVA revealed significant differences between age group and anterograde F(2,4197) = 107.449, p < .001 and retrograde amnesia F(2,4197) = 82.949, p < .001. Bonferroni pairwise comparison revealed the adolescent athlete group experienced more concussions that result in both anterograde and retrograde amnesia compared to young and adult athletes. There was no significant difference between young and adult athletes. Additionally, there is a significant difference regarding total games missed following concussion F(2,4197) = 117.723, p < .001, with adolescent athletes missing more games compared to young and adult athletes. Conclusions The findings of this study suggest adolescent athletes tend to experience more amnesia-related concussions compared to young and adult athletes. Additionally, adolescent athletes miss more games following these types of concussions. This study highlights the impact that certain types of concussions have on athletes across the developmental stages. Future research should analyze the cognitive effects of various types of concussions across the developmental stages.

Evaluation of Appropriate Reference Genes For Investigating Gene Expression in Chlorops oryzae (Diptera: Chloropidae)

2019 ◽  
Vol 112 (5) ◽  
pp. 2207-2214 ◽  
Author(s):  
Ping Tian ◽  
Lin Qiu ◽  
Ailin Zhou ◽  
Guo Chen ◽  
Hualiang He ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ribosomal Protein ◽  
Reference Genes ◽  
Molecular Mechanisms ◽  
Developmental Stages ◽  
Quantitative Polymerase Chain Reaction ◽  
Housekeeping Genes ◽  
Future Research ◽  
Economic Damage ◽  
Physiological Processes

Abstract Reverse transcription quantitative polymerase chain reaction (PCR) has become an invaluable technique for analyzing gene expression in many insects. However, this approach requires the use of stable reference genes to normalize the data. Chlorops oryzae causes significant economic damage to rice crops throughout Asia. The lack of suitable reference genes has hindered research on the molecular mechanisms underlying many physiological processes of this species. In this study, we used quantitative real-time PCR to evaluate the expression of eight C. oryzae housekeeping genes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-actin (βACT), beta-tubulin (βTUB), Delta Elongation factor-1 (EF1δ), ribosomal protein S11 (RPS11), RPS15, C-terminal-Binding Protein (CtBP), and ribosomal protein 49 (RP49) in different developmental stages and tissues in both larvae and adults. We analyzed the data with four different software packages: geNorm, NormFinder, BestKeeper, and RefFinder and compared the results obtained with each method. The results indicate that PRS15 and RP49 can be used as stable reference genes for quantifying gene expression in different developmental stages and larval tissues. GAPDH and βACT, which have been considered stable reference genes by previous studies, were the least stable of the candidate genes with respect to larval tissues. GAPDH was, however, the most stable reference gene for adult tissues. We verified the candidate reference genes identified and found that the expression levels of Cadherins (Cads) changed when different reference genes were used to normalize gene expression. This study provides a valuable foundation for future research on gene function, and investigating the molecular basis of physiological processes, in C. oryzae.

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