scholarly journals A branching process model for the analysis of abortive colony size distributions in carbon ion-irradiated normal human fibroblasts

2014 ◽  
Vol 55 (3) ◽  
pp. 423-431 ◽  
Author(s):  
T. Sakashita ◽  
N. Hamada ◽  
I. Kawaguchi ◽  
T. Hara ◽  
Y. Kobayashi ◽  
...  
Keyword(s):  
Colony Size ◽  
Process Model ◽  
Branching Process ◽  
Human Fibroblasts ◽  
Size Distributions ◽  
Carbon Ion ◽  
Normal Human

scholarly journals A Framework for Analysis of Abortive Colony Size Distributions Using a Model of Branching Processes in Irradiated Normal Human Fibroblasts

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e70291 ◽  
Author(s):  
Tetsuya Sakashita ◽  
Nobuyuki Hamada ◽  
Isao Kawaguchi ◽  
Noriyuki B. Ouchi ◽  
Takamitsu Hara ◽  
...  
Keyword(s):  
Colony Size ◽  
Branching Processes ◽  
Human Fibroblasts ◽  
Size Distributions ◽  
Normal Human

Expression profiles are different in carbon ion-irradiated normal human fibroblasts and their bystander cells

2008 ◽  
Vol 642 (1-2) ◽  
pp. 57-67 ◽  
Author(s):  
Mayumi Iwakawa ◽  
Nobuyuki Hamada ◽  
Kaori Imadome ◽  
Tomoo Funayama ◽  
Testuya Sakashita ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Human Fibroblasts ◽  
Carbon Ion ◽  
Normal Human ◽  
Bystander Cells

scholarly journals Precursor-Boosted Production of Metabolites in Nasturtium officinale Microshoots Grown in Plantform Bioreactors, and Antioxidant and Antimicrobial Activities of Biomass Extracts

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4660
Author(s):  
Marta Klimek-Szczykutowicz ◽  
Michał Dziurka ◽  
Ivica Blažević ◽  
Azra Đulović ◽  
Małgorzata Miazga-Karska ◽  
...  
Keyword(s):  
Photosynthetic Pigments ◽  
Human Fibroblasts ◽  
Antimicrobial Activities ◽  
Precursor Feeding ◽  
Nasturtium Officinale ◽  
Qualitative And Quantitative ◽  
Normal Human ◽  
Inhibition Zones ◽  
Ferulic Acids ◽  
Antioxidant And Antimicrobial Activities

The study demonstrated the effects of precursor feeding on the production of glucosinolates (GSLs), flavonoids, polyphenols, saccharides, and photosynthetic pigments in Nasturtium officinale microshoot cultures grown in Plantform bioreactors. It also evaluated the antioxidant and antimicrobial activities of extracts. L-phenylalanine (Phe) and L-tryptophan (Trp) as precursors were tested at 0.05, 0.1, 0.5, 1.0, and 3.0 mM. They were added at the beginning (day 0) or on day 10 of the culture. Microshoots were harvested after 20 days. Microshoots treated with 3.0 mM Phe (day 0) had the highest total GSL content (269.20 mg/100 g DW). The qualitative and quantitative profiles of the GSLs (UHPLC-DAD-MS/MS) were influenced by precursor feeding. Phe at 3.0 mM stimulated the best production of 4-methoxyglucobrassicin (149.99 mg/100 g DW) and gluconasturtiin (36.17 mg/100 g DW). Total flavonoids increased to a maximum of 1364.38 mg/100 g DW with 3.0 mM Phe (day 0), and polyphenols to a maximum of 1062.76 mg/100 g DW with 3.0 mM Trp (day 0). The precursors also increased the amounts of p-coumaric and ferulic acids, and rutoside, and generally increased the production of active photosynthetic pigments. Antioxidant potential increased the most with 0.1 mM Phe (day 0) (CUPRAC, FRAP), and with 0.5 mM Trp (day 10) (DPPH). The extracts of microshoots treated with 3.0 mM Phe (day 0) showed the most promising bacteriostatic activity against microaerobic Gram-positive acne strains (MIC 250–500 µg/mL, 20–21 mm inhibition zones). No extract was cytotoxic to normal human fibroblasts over the tested concentration range (up to 250 μg/mL).

scholarly journals Generation of nonviral integration-free human iPS cell line KISCOi001-A from normal human fibroblasts, under defined xeno-free and feeder-free conditions

2021 ◽  
Vol 51 ◽  
pp. 102193
Author(s):  
Jose Inzunza ◽  
Jonathan Arias-Fuenzalida ◽  
Juan Segura-Aguilar ◽  
Ivan Nalvarte ◽  
Mukesh Varshney
Keyword(s):  
Cell Line ◽  
Human Fibroblasts ◽  
Ips Cell ◽  
Normal Human

scholarly journals The Probability of Fixation in Populations of Changing Size

Genetics ◽  
1997 ◽  
Vol 146 (2) ◽  
pp. 723-733 ◽  
Author(s):  
Sarah P Otto ◽  
Michael C Whitlock
Keyword(s):  
Diffusion Equation ◽  
Adaptive Evolution ◽  
Process Model ◽  
Branching Process ◽  
Approximate Solutions ◽  
Logistic Growth ◽  
Beneficial Mutations ◽  
Demographic Models ◽  
Deleterious Alleles ◽  
Probability Of Fixation

The rate of adaptive evolution of a population ultimately depends on the rate of incorporation of beneficial mutations. Even beneficial mutations may, however, be lost from a population since mutant individuals may, by chance, fail to reproduce. In this paper, we calculate the probability of fixation of beneficial mutations that occur in populations of changing size. We examine a number of demographic models, including a population whose size changes once, a population experiencing exponential growth or decline, one that is experiencing logistic growth or decline, and a population that fluctuates in size. The results are based on a branching process model but are shown to be approximate solutions to the diffusion equation describing changes in the probability of fixation over time. Using the diffusion equation, the probability of fixation of deleterious alleles can also be determined for populations that are changing in size. The results developed in this paper can be used to estimate the fixation flux, defined as the rate at which beneficial alleles fix within a population. The fixation flux measures the rate of adaptive evolution of a population and, as we shall see, depends strongly on changes that occur in population size.

Branching processes and functional differential equations determining steady-size distributions in cell populations

1995 ◽  
Vol 32 (01) ◽  
pp. 1-10
Author(s):  
Ziad Taib
Keyword(s):  
Differential Equation ◽  
Branching Process ◽  
Branching Processes ◽  
Functional Differential Equations ◽  
Cell Populations ◽  
Size Distributions ◽  
Multitype Branching Process ◽  
Functional Differential ◽  
Intuitive Interpretation ◽  
Infinite Sum

The functional differential equation y′(x) = ay(λx) + by(x) arises in many different situations. The purpose of this note is to show how it arises in some multitype branching process cell population models. We also show how its solution can be given an intuitive interpretation as the probability density function of an infinite sum of independent but not identically distributed random variables.

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