A simple method for environmental cell depressurization for use with an electron microscope

Microscopy ◽  
2017 ◽  
Vol 66 (6) ◽  
pp. 424-430
Author(s):  
Naoki Ogawa ◽  
Ryo Mizokawa ◽  
Minoru Saito ◽  
Akira Ishikawa
1973 ◽  
Vol 97 (1-2) ◽  
pp. 209-216 ◽  
Author(s):  
D. L. Allinson ◽  
M. S. Loveday ◽  
A. W. O. Gosnold

2005 ◽  
Vol 475-479 ◽  
pp. 4029-4034 ◽  
Author(s):  
Katsuhiro Sasaki ◽  
Hiroyasu Saka

A novel method to observe the electrostatic field distribution with a conventional transmission electron microscope has been developed. The method allows measurements of a potential difference less than 1V/µm. This method can be performed in any kind of conventional transmission electron microscope and applied to the observation of the electric/magnetic field at the level of a specimen.


2012 ◽  
Vol 486 ◽  
pp. 412-416 ◽  
Author(s):  
Ming Ge Zhou ◽  
Wei Li ◽  
Ming Gang Zhu ◽  
Dong Zhou ◽  
Yang Long Hou

FePt thin films with perpendicularc-axis orientation have potential for application as magnetic media for data storage, In order to be used as perpendicular magnetic storage media,The chemically synthesized cubic FePt nanoparticles is easier to assembed oriented nanoarray, especially the cubic FePt nanoparticles with shape anisotropy is expected to trigger magnetic anisotropy.In this study we provide a simple method to synthesize single crystalline FePt nanoparticles with cubic shapes.The key factor to formation of cubic morphology is using Mo (Co)6as a reducing agent. As-synthesized FePt nanocubes with size that are close to 7-10nm The self-assembly FePt nanocubes are chemically disordered with face centre cubic (fcc) structure where as after annealing these particles changed to face centre tetragonal (fct) ordered structure. The phase analysis, structure, and magnetic properties were determined by X-ray diffraction (XRD), High resolution transmission electron microscope (HRTEM), Scanning electron microscope (SEM) and vibrating sample magnetometer (VSM) techniques.


Author(s):  
F. Nagata ◽  
I. Ishikawa

During these thirty years, some experiments have been made to observe living materials in an electron microscope. Observation of living things will prove whether biological tissues found in microscope images of sectioned specimens are same as that of living material, and also will enable us to observe organic functions dynamically.The authers studied the possibility of the observation of living materials. There are three important subjects for the accomplishment; (1) development of environmental cell, (2) specimen injury with electrons irradiation, (3) improvement of image contrast for wet specimen.It is generally expected that injury to living material will be reduced as an accelerating voltage of irradiating electrons is increased. But neither the rate nor the effects has been studied precisely. In this studies, dry spores of Bacillus megaterium were irradiated with electrons in a specimen chamber of an electron microscope. The accelerating voltage was in the region of 25 to 750 kV and electron quantity was 1x10-7 to 1xl0-2 coulombs/cm2 on a specimen. After the irradiation, the specimens were incubated in the culture fluid and then they were observed with an electron microscope. It is found that as electron number increase, new vegetable cells cease to exist, moreover monsters occur and then spores do not swell. They are similar in tendency at each accelerating voltage but different in amount of irradiation.


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