B12-P-13An In-Situ Environmental Cell-Holder of Conventional Transmission Electron Microscope and Its Applications

Microscopy ◽  
2015 ◽  
Vol 64 (suppl 1) ◽  
pp. i91.2-i91
Author(s):  
Yongming Wang ◽  
Takenobu Wakasugi ◽  
Hiroki Nagakura ◽  
Shigehito Isobe ◽  
Naoyuki Hashimoto ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Transmission Electron ◽  
Environmental Cell ◽  
Conventional Transmission Electron Microscope

Contrast Considerations in Environmental Cells

1973 ◽  
Vol 31 ◽  
pp. 20-21
Author(s):  
J. R. Sellar
Keyword(s):  
Electron Microscope ◽  
Multiple Scattering ◽  
Chromatic Aberration ◽  
Objective Lens ◽  
Noise Effects ◽  
Transmission Electron ◽  
Environmental Cell ◽  
A Cell ◽  
Conventional Transmission Electron Microscope

To study chemical reactions in situ and observe biological specimens in the hydrated state, it is necessary to isolate the specimen from the vacuum of the electron microscope by means of a cell. Figure 1 represents such a cell in a conventional transmission electron microscope (CTEM) with apertures or windows A, specimen mounting B, and specimen T. O labels the objective lens, L the cell gas, and S is the width of the layer of gas.Calculations have been carried out involving the contrast available when thick specimens are imaged or an environmental cell is used at 1000 keV. For the limitations considered, including multiple scattering, chromatic aberration and the effects of various geometrical configurations, it appears that, in the absence of noise effects due to loss of intensity, multiple scattering causes the largest disc of confusion.

In Situ Environmental Cell–Transmission Electron Microscopy Study of Microbial Reduction of Chromium(VI) Using Electron Energy Loss Spectroscopy

2001 ◽  
Vol 7 (6) ◽  
pp. 470-485 ◽  
Author(s):  
Tyrone L. Daulton ◽  
Brenda J. Little ◽  
Kristine Lowe ◽  
Joanne Jones-Meehan
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Electron Microscope ◽  
Energy Loss ◽  
Transmission Electron Microscope ◽  
Transmission Electron ◽  
Environmental Cell ◽  
Cell Transmission ◽  
Electron Energy Loss

AbstractReduction of Cr(VI) by the bacterium, Shewanella oneidensis (previously classified Shewanella putrefaciens strain MR-1), was studied by absorption spectrophotometry and in situ, environmental cell–transmission electron microscopy (EC-TEM) coupled with electron energy loss spectroscopy (EELS). Bacteria from rinsed cultures were placed directly in the environmental cell of the transmission electron microscope and examined under 100 Torr pressure. Bright field EC-TEM images show two distinct populations of S. oneidensis in incubated cultures containing Cr(VI)O42−: those that exhibit low image contrast and heavily precipitateencrusted cells exhibiting high image contrast. Several EELS techniques were applied to determine the oxidation state of Cr associated with encrusted cells. The encrusted cells are shown to contain a reduced form of Cr in oxidation state +3 or lower. These results demonstrate the capability to determine the chemistry and valence state of reduction products associated with unfixed, hydrated bacteria in an environmental cell transmission electron microscope.

Remote On-Line Control of a High-Voltage in situ Transmission Electron Microscope with A Rational User Interface

1996 ◽  
Vol 54 ◽  
pp. 384-385
Author(s):  
M.A. O’Keefe ◽  
J. Taylor ◽  
D. Owen ◽  
B. Crowley ◽  
K.H. Westmacott ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
User Interface ◽  
Transmission Electron Microscope ◽  
High Voltage ◽  
Materials Science ◽  
Transmission Electron ◽  
On Line ◽  
Electron Microscopes

Remote on-line electron microscopy is rapidly becoming more available as improvements continue to be developed in the software and hardware of interfaces and networks. Scanning electron microscopes have been driven remotely across both wide and local area networks. Initial implementations with transmission electron microscopes have targeted unique facilities like an advanced analytical electron microscope, a biological 3-D IVEM and a HVEM capable of in situ materials science applications. As implementations of on-line transmission electron microscopy become more widespread, it is essential that suitable standards be developed and followed. Two such standards have been proposed for a high-level protocol language for on-line access, and we have proposed a rational graphical user interface. The user interface we present here is based on experience gained with a full-function materials science application providing users of the National Center for Electron Microscopy with remote on-line access to a 1.5MeV Kratos EM-1500 in situ high-voltage transmission electron microscope via existing wide area networks. We have developed and implemented, and are continuing to refine, a set of tools, protocols, and interfaces to run the Kratos EM-1500 on-line for collaborative research. Computer tools for capturing and manipulating real-time video signals are integrated into a standardized user interface that may be used for remote access to any transmission electron microscope equipped with a suitable control computer.

scholarly journals In-Situ Stretching Patterned Graphene Nanoribbons in the Transmission Electron Microscope

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Zhongquan Liao ◽  
Leonardo Medrano Sandonas ◽  
Tao Zhang ◽  
Martin Gall ◽  
Arezoo Dianat ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Graphene Nanoribbons ◽  
Transmission Electron
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