scholarly journals Rapid immunohistochemical diagnosis of tobacco mosaic virus disease by microwave-assisted plant sample preparation

Microscopy ◽  
2013 ◽  
Vol 62 (5) ◽  
pp. 547-553 ◽  
Author(s):  
Günther Zellnig ◽  
Stefan Möstl ◽  
Bernd Zechmann
Keyword(s):  
Sample Preparation ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Disease ◽  
Plant Sample ◽  
Microwave Assisted ◽  
Immunohistochemical Diagnosis

scholarly journals Serological Detection of Tobacco Mosaic Virus and Cucumber Mosaic Virus Infecting Tomato (Solanum Lycopersicum) Using a Lateral Flow Immunoassay Technique

2013 ◽  
Vol 1 (2) ◽  
pp. 102 ◽  
Author(s):  
Joseph Nii Lante Lamptey ◽  
Michael Kwabena Osei ◽  
Moses Brandford Mochiah ◽  
Kingsley Osei ◽  
Joseph Nketiah Berchie ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Disease ◽  
Tomato Leaf ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Immunoassay Technique ◽  
Tomato Leaf Curl ◽  
Leaf Curl

A study was conducted to determine the prevalence of Cucumber mosaic virus (CMV) and Tobacco mosaic virus (TMV) in some major tomato growing areas in Ghana as part of a comprehensive strategy for the management of viral diseases on tomato. The lateral flow immunoassay technique (the immunostrip test of Agdia Inc.) was used to assay for TMV and CMV in samples with symptoms of virus infection collected from some major tomato growing areas in Ghana; Vea, Tono and Pwalugu (Upper East region-UE), Agogo and Akumadan (Ashanti region-AR) and Tanoso and Tuobodom (Brong-Ahafo region-BA) in 2011 and 2012. In the UE, TMV and CMV were both detected at Vea, Tono and Pwalugu. In the AR, CMV and TMV were both detected at Akumadan while only CMV was detected at Agogo. In BA, TMV and CMV were both detected at Tanoso and Tuobodom.  Field incidence of TMV was higher in the UE which had higher average daily temperatures. The serological identification of TMV in the three regions is the first of this virus in Ghana.  This is also the first report of the use of this lateral flow immunoassay technique to detect these viruses in the country. The identification of TMV and CMV in Ghana gives an indication of the need to focus efforts for virus disease management on these virus in addition to the already known begomoviruses like Tomato yellow leaf curl Mali virus (TYLCMV)(Delete), Tomato leaf curl Kumasi virus and Tomato leaf curl Ghana virus. 

Quantitative Measurements on the Ion Etching of TMV

1973 ◽  
Vol 31 ◽  
pp. 336-337
Author(s):  
Irwin Bendet ◽  
Nabil Rizk
Keyword(s):  
Electron Microscope ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Ion Current ◽  
Beam Diameter ◽  
Ion Etching ◽  
Preliminary Results ◽  
Quantitative Measurements ◽  
Monitoring Devices

Preliminary results reported last year on the ion etching of tobacco mosaic virus indicated that the diameter of the virus decreased more rapidly at 10KV than at 5KV, perhaps reaching a constant value before disappearing completely.In order to follow the effects of ion etching on TMV more quantitatively we have designed and built a second apparatus (Fig. 1), which incorporates monitoring devices for measuring ion current and vacuum as well as accelerating voltage. In addition, the beam diameter has been increased to approximately 1 cm., so that ten electron microscope grids can be exposed to the beam simultaneously.

Tobacco Mosaic Virus-Infected Tissue

1985 ◽  
Vol 43 ◽  
pp. 510-511
Author(s):  
Egbert W. Henry
Keyword(s):  
Nicotiana Tabacum ◽  
Tobacco Mosaic Virus ◽  
Chlorogenic Acid ◽  
Mosaic Virus ◽  
Host Resistance ◽  
Oxidative Metabolism ◽  
Leaf Tissue ◽  
Vein Clearing ◽  
Basal Septum ◽  
Concentration Levels

Tobacco mosaic virus (TMV) infection has been studied in several investigations of Nicotiana tabacum leaf tissue. Earlier studies have suggested that TMV infection does not have precise infective selectivity vs. specific types of tissues. Also, such tissue conditions as vein banding, vein clearing, liquification and suberization may result from causes other than direct TMV infection. At the present time, it is thought that the plasmodesmata, ectodesmata and perhaps the plasmodesmata of the basal septum may represent the actual or more precise sites of TMV infection.TMV infection has been implicated in elevated levels of oxidative metabolism; also, TMV infection may have a major role in host resistance vs. concentration levels of phenolic-type enzymes. Therefore, enzymes such as polyphenol oxidase, peroxidase and phenylalamine ammonia-lyase may show an increase in activity in response to TMV infection. It has been reported that TMV infection may cause a decrease in o-dihydric phenols (chlorogenic acid) in some tissues.

Nitroxyl Modified Tobacco Mosaic Virus as a Metal-Free High-Relaxivity MRI and EPR Active Superoxide Sensor

2018 ◽  
Author(s):  
Madushani Dharmarwardana ◽  
André F. Martins ◽  
Zhuo Chen ◽  
Philip M. Palacios ◽  
Chance M. Nowak ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Single Molecule ◽  
Biological Fluids ◽  
Cellular Respiration ◽  
Organic Radical ◽  
Paramagnetic Resonance ◽  
Metal Free ◽  
Disease States

Superoxide overproduction is known to occur in multiple disease states requiring critical care yet non-invasive detection of superoxide in deep tissue remains a challenge. Herein, we report a metal-free magnetic resonance imaging (MRI) and electron paramagnetic resonance (EPR) active contrast agent prepared by “click conjugating” paramagnetic organic radical contrast agents (ORCAs) to the surface of tobacco mosaic virus (TMV). While ORCAs are known to be reduced <i>in vivo</i> to an MRI/EPR silent state, their oxidation is facilitated specifically by reactive oxygen species—in particular superoxide—and are largely unaffected by peroxides and molecular oxygen. Unfortunately, single molecule ORCAs typically offer weak MRI contrast. In contrast, our data confirm that the macromolecular ORCA-TMV conjugates show marked enhancement for <i>T<sub>1</sub></i> contrast at low field (<3.0 T), and <i>T<sub>2</sub></i> contrast at high field (9.4 T). Additionally, we demonstrated that the unique topology of TMV allows for “quenchless fluorescent” bimodal probe for concurrent fluorescence and MRI/EPR imaging, which was made possible by exploiting the unique inner and outer surface of the TMV nanoparticle. <a>Finally, we show TMV-ORCAs do not respond to normal cellular respiration, minimizing the likelihood for background, yet still respond to enzymatically produced superoxide in complicated biological fluids like serum.</a>

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