A useful method for observing intracellular structures of free and cultured cells by scanning electron microscopy

Microscopy ◽  
2012 ◽  
Vol 61 (2) ◽  
pp. 105-111 ◽  
Author(s):  
Daisuke Koga ◽  
Masato Nakajima ◽  
Tatsuo Ushiki
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cultured Cells ◽  
Scanning Electron

scholarly journals 3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy

2016 ◽  
Vol 130 (1) ◽  
pp. 278-291 ◽  
Author(s):  
Matthew R. G. Russell ◽  
Thomas R. Lerner ◽  
Jemima J. Burden ◽  
David O. Nkwe ◽  
Annegret Pelchen-Matthews ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cultured Cells ◽  
Light And Electron Microscopy ◽  
Scanning Electron

scholarly journals A modified ‘NanoSuit®’ preserves wet samples in high vacuum: direct observations on cells and tissues in field-emission scanning electron microscopy

2017 ◽  
Vol 4 (3) ◽  
pp. 160887 ◽  
Author(s):  
Yasuharu Takaku ◽  
Hiroshi Suzuki ◽  
Hideya Kawasaki ◽  
Isao Ohta ◽  
Daisuke Ishii ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Field Emission ◽  
Structural Changes ◽  
Cultured Cells ◽  
High Vacuum ◽  
Insect Larvae ◽  
Direct Observations ◽  
High Vacuum Environment ◽  
Scanning Electron

Although field-emission scanning electron microscopy (FE-SEM) has proven very useful in biomedical research, the high vacuum required (10 −3 to 10 −7  Pa) precludes direct observations of living cells and tissues at high resolution and often produces unwanted structural changes. We have previously described a method that allows the investigator to keep a variety of insect larvae alive in the high vacuum environment of the electron microscope by encasing the organisms in a thin, vacuum-proof suit, the ‘NanoSuit®'. However, it was impossible to protect wet tissues freshly excised from intact organisms or cultured cells. Here we describe an improved ‘NanoSuit' technique to overcome this limitation. We protected the specimens with a surface shield enhancer (SSE) solution that consists of glycerine and electrolytes and found that the fine structure of the SSE-treated specimens is superior to that of conventionally prepared specimens. The SSE-based NanoSuit affords a much stronger barrier to gas and/or liquid loss than the previous NanoSuit did and, since it allows more detailed images, it could significantly help to elucidate the ‘real' organization of cells and their functions.

Scanning Electron Microscopy of Ethanol Infiltrated Freeze-Fractured Cell Pellets

1974 ◽  
Vol 32 ◽  
pp. 98-99
Author(s):  
William G. Henk ◽  
Ben O. Spurlock
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Critical Point ◽  
Freeze Drying ◽  
Cultured Cells ◽  
Resolving Power ◽  
Ice Crystal ◽  
Internal Structures ◽  
Critical Point Drying ◽  
Scanning Electron

The increased depth of focus and superior resolving power of the scanning electron microscope provide advantages over the light microscope in viewing the external morphology of cultured cells and protists. Internal structures have, however, proved more difficult to observe. Freeze drying adequately preserves surface structures but results in poorly preserved cytoplasmic elements due to ice crystal damage. Critical point drying results in good preservation of both surface and cytoplasmic fine structure. Attempts to cut or break critical point dried material, however, result in plastic deformation of the cells. Humphreys, et al, recently introduced freeze fracturing of ethanol infiltrated tissues for biological scanning electron microscopy. We have modified and applied their technique and obtained similar results with Paramecium sp. obtained from mass cultures.

A method for processing free-floating cultured cells for scanning electron microscopy

1982 ◽  
Vol 7 (3) ◽  
pp. 85-88 ◽  
Author(s):  
James A. McAteer ◽  
Andrew P. Evan ◽  
William H. J. Douglas
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cultured Cells ◽  
Scanning Electron
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