High-contrast imaging of plastic-embedded tissues by phase contrast electron microscopy

2009 ◽  
Vol 58 (2) ◽  
pp. 35-45 ◽  
Author(s):  
K. Atsuzawa ◽  
N. Usuda ◽  
A. Nakazawa ◽  
M. Fukasawa ◽  
R. Danev ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Phase Contrast ◽  
High Contrast ◽  
Contrast Imaging ◽  
High Contrast Imaging

scholarly journals High contrast imaging and thickness determination of graphene with in-column secondary electron microscopy

2011 ◽  
Vol 110 (1) ◽  
pp. 014315 ◽  
Author(s):  
Vidya Kochat ◽  
Atindra Nath Pal ◽  
E. S. Sneha ◽  
Arjun Sampathkumar ◽  
Anshita Gairola ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Secondary Electron ◽  
High Contrast ◽  
Contrast Imaging ◽  
Thickness Determination ◽  
High Contrast Imaging

scholarly journals 3P-331 The application of phase electron microscopy to plastic embedded tissues for a high contrast imaging(The 46th Annual Meeting of the Biophysical Society of Japan)

2008 ◽  
Vol 48 (supplement) ◽  
pp. S178
Author(s):  
Kimie Atsuzawa ◽  
Nobuteru Usuda ◽  
Ayami Nakazawa ◽  
Danev Radostin ◽  
Shouzou Sugitani ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Annual Meeting ◽  
High Contrast ◽  
Contrast Imaging ◽  
High Contrast Imaging ◽  
Biophysical Society

scholarly journals High-Contrast Imaging of Nanodiamonds in Cells by Energy Filtered and Correlative Light-Electron Microscopy: Toward a Quantitative Nanoparticle-Cell Analysis

Nano Letters ◽  
2019 ◽  
Vol 19 (3) ◽  
pp. 2178-2185 ◽  
Author(s):  
Shen Han ◽  
Marco Raabe ◽  
Lorna Hodgson ◽  
Judith Mantell ◽  
Paul Verkade ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cell Analysis ◽  
High Contrast ◽  
Contrast Imaging ◽  
High Contrast Imaging ◽  
In Cells

The CM120 Biotwin: a high-contrast objective lens, optimum cryo-vacuum and improved EDX performance

1995 ◽  
Vol 53 ◽  
pp. 584-585
Author(s):  
Uwe Lücken ◽  
Michael Felsmann ◽  
Wim M. Busing ◽  
Frank de Jong
Keyword(s):  
Life Science ◽  
Focal Length ◽  
Objective Lens ◽  
High Contrast ◽  
Contrast Imaging ◽  
X Ray ◽  
Forming Mechanism ◽  
Similar Image ◽  
High Contrast Imaging ◽  
Low Concentrations

A new microscope for the study of life science specimen has been developed. Special attention has been given to the problems of unstained samples, cryo-specimens and x-ray analysis at low concentrations.A new objective lens with a Cs of 6.2 mm and a focal length of 5.9 mm for high-contrast imaging has been developed. The contrast of a TWIN lens (f = 2.8 mm, Cs = 2 mm) and the BioTWTN are compared at the level of mean and SD of slow scan CCD images. Figure 1a shows 500 +/- 150 and Fig. 1b only 500 +/- 40 counts/pixel. The contrast-forming mechanism for amplitude contrast is dependent on the wavelength, the objective aperture and the focal length. For similar image conditions (same voltage, same objective aperture) the BioTWIN shows more than double the contrast of the TWIN lens. For phasecontrast specimens (like thin frozen-hydrated films) the contrast at Scherzer focus is approximately proportional to the √ Cs.

scholarly journals Anti-quenching NIR-II molecular fluorophores for in vivo high-contrast imaging and pH sensing

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Shangfeng Wang ◽  
Yong Fan ◽  
Dandan Li ◽  
Caixia Sun ◽  
Zuhai Lei ◽  
...  
Keyword(s):  
Ph Sensing ◽  
High Contrast ◽  
Contrast Imaging ◽  
High Contrast Imaging
Sign in / Sign up

Export Citation Format

Share Document