scholarly journals Histone H4 deacetylation facilitates 53BP1 DNA damage signaling and double-strand break repair

2013 ◽  
Vol 5 (3) ◽  
pp. 157-165 ◽  
Author(s):  
K.-Y. Hsiao ◽  
C. A. Mizzen
Keyword(s):  
Dna Damage ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Histone H4 ◽  
Dna Damage Signaling ◽  
Break Repair ◽  
Histone H4 Deacetylation

scholarly journals MOF and Histone H4 Acetylation at Lysine 16 Are Critical for DNA Damage Response and Double-Strand Break Repair

2010 ◽  
Vol 30 (14) ◽  
pp. 3582-3595 ◽  
Author(s):  
Girdhar G. Sharma ◽  
Sairei So ◽  
Arun Gupta ◽  
Rakesh Kumar ◽  
Christelle Cayrou ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Damage Response ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Histone H4 ◽  
Dsb Repair ◽  
Altered Expression ◽  
Damage Response ◽  
Break Repair

ABSTRACT The human MOF gene encodes a protein that specifically acetylates histone H4 at lysine 16 (H4K16ac). Here we show that reduced levels of H4K16ac correlate with a defective DNA damage response (DDR) and double-strand break (DSB) repair to ionizing radiation (IR). The defect, however, is not due to altered expression of proteins involved in DDR. Abrogation of IR-induced DDR by MOF depletion is inhibited by blocking H4K16ac deacetylation. MOF was found to be associated with the DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a protein involved in nonhomologous end-joining (NHEJ) repair. ATM-dependent IR-induced phosphorylation of DNA-PKcs was also abrogated in MOF-depleted cells. Our data indicate that MOF depletion greatly decreased DNA double-strand break repair by both NHEJ and homologous recombination (HR). In addition, MOF activity was associated with general chromatin upon DNA damage and colocalized with the synaptonemal complex in male meiocytes. We propose that MOF, through H4K16ac (histone code), has a critical role at multiple stages in the cellular DNA damage response and DSB repair.

scholarly journals Xbp1-mediated histone H4 deacetylation contributes to DNA double-strand break repair in yeast

Cell Research ◽  
2011 ◽  
Vol 21 (11) ◽  
pp. 1619-1633 ◽  
Author(s):  
Ran Tao ◽  
Hua Chen ◽  
Chan Gao ◽  
Peng Xue ◽  
Fuquan Yang ◽  
...  
Keyword(s):  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Histone H4 ◽  
Dna Double Strand Break ◽  
Break Repair ◽  
Histone H4 Deacetylation

scholarly journals The Caenorhabditis elegans Homolog of Gen1/Yen1 Resolvases Links DNA Damage Signaling to DNA Double-Strand Break Repair

PLoS Genetics ◽  
2010 ◽  
Vol 6 (7) ◽  
pp. e1001025 ◽  
Author(s):  
Aymeric P. Bailly ◽  
Alasdair Freeman ◽  
Julie Hall ◽  
Anne-Cécile Déclais ◽  
Arno Alpi ◽  
...  
Keyword(s):  
Dna Damage ◽  
Caenorhabditis Elegans ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Dna Damage Signaling ◽  
Dna Double Strand Break ◽  
Break Repair

scholarly journals The nucleoporin 153, a novel factor in double-strand break repair and DNA damage response

Oncogene ◽  
2012 ◽  
Vol 31 (45) ◽  
pp. 4803-4809 ◽  
Author(s):  
C Lemaître ◽  
B Fischer ◽  
A Kalousi ◽  
A-S Hoffbeck ◽  
J Guirouilh-Barbat ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Damage Response ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Damage Response ◽  
Break Repair

scholarly journals Involvement of POLA2 in Double Strand Break Repair and Genotoxic Stress

2020 ◽  
Vol 21 (12) ◽  
pp. 4245
Author(s):  
Tuyen T. Dang ◽  
Julio C. Morales
Keyword(s):  
Dna Damage ◽  
Genomic Instability ◽  
Dna Polymerase ◽  
Genotoxic Stress ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Dsb Repair ◽  
Dna Polymerase Alpha ◽  
Break Repair

Cellular survival is dependent on the efficient replication and transmission of genomic information. DNA damage can be introduced into the genome by several different methods, one being the act of DNA replication. Replication is a potent source of DNA damage and genomic instability, especially through the formation of DNA double strand breaks (DSBs). DNA polymerase alpha is responsible for replication initiation. One subunit of the DNA polymerase alpha replication machinery is POLA2. Given the connection between replication and genomic instability, we decided to examine the role of POLA2 in DSB repair, as little is known about this topic. We found that loss of POLA2 leads to an increase in spontaneous DSB formation. Loss of POLA2 also slows DSB repair kinetics after treatment with etoposide and inhibits both of the major double strand break repair pathways: non-homologous end-joining and homologous recombination. In addition, loss of POLA2 leads to increased sensitivity to ionizing radiation and PARP1 inhibition. Lastly, POLA2 expression is elevated in glioblastoma multiforme tumors and correlates with poor overall patient survival. These data demonstrate a role for POLA2 in DSB repair and resistance to genotoxic stress.

Sign in / Sign up

Export Citation Format

Share Document