scholarly journals Gene Expression Profiles of Heat Shock Proteins 70 and 90 From Empoasca onukii (Hemiptera: Cicadellidae) in Response to Temperature Stress

2015 ◽  
Vol 15 (1) ◽  
pp. 49-49 ◽  
Author(s):  
L. Qiao ◽  
J. X. Wu ◽  
D. Z. Qin ◽  
X. C. Liu ◽  
Z. C. Lu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Temperature Stress ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Shock Proteins ◽  
Response To Temperature

scholarly journals Gene Expression Profiles Suggest a Better Cold Acclimation of Polyploids in the Alpine Species Ranunculus kuepferi (Ranunculaceae)

Genes ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1818
Author(s):  
Eleni Syngelaki ◽  
Claudia Paetzold ◽  
Elvira Hörandl
Keyword(s):  
Gene Expression ◽  
Cold Stress ◽  
Cold Acclimation ◽  
Temperature Stress ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Distribution Patterns ◽  
Gene Set ◽  
Cation Homeostasis ◽  
Response To Temperature

Alpine habitats are shaped by harsh abiotic conditions and cold climates. Temperature stress can affect phenotypic plasticity, reproduction, and epigenetic profiles, which may affect acclimation and adaptation. Distribution patterns suggest that polyploidy seems to be advantageous under cold conditions. Nevertheless, whether temperature stress can induce gene expression changes in different cytotypes, and how the response is initialized through gene set pathways and epigenetic control remain vague for non-model plants. The perennial alpine plant Ranunculus kuepferi was used to investigate the effect of cold stress on gene expression profiles. Diploid and autotetraploid individuals were exposed to cold and warm conditions in climate growth chambers and analyzed via transcriptome sequencing and qRT-PCR. Overall, cold stress changed gene expression profiles of both cytotypes and induced cold acclimation. Diploids changed more gene set pathways than tetraploids, and suppressed pathways involved in ion/cation homeostasis. Tetraploids mostly activated gene set pathways related to cell wall and plasma membrane. An epigenetic background for gene regulation in response to temperature conditions is indicated. Results suggest that perennial alpine plants can respond to temperature extremes via altered gene expression. Tetraploids are better acclimated to cold conditions, enabling them to colonize colder climatic areas in the Alps.

scholarly journals Gene Expression in Porphyromonas gingivalis after Contact with Human Epithelial Cells

2005 ◽  
Vol 73 (4) ◽  
pp. 2327-2335 ◽  
Author(s):  
Yumiko Hosogi ◽  
Margaret J. Duncan
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Porphyromonas Gingivalis ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Cell Types ◽  
Adverse Conditions ◽  
Human Epithelial Cells ◽  
Host Epithelium ◽  
Shock Proteins

ABSTRACT Porphyromonas gingivalis, a gram-negative oral anaerobe, is strongly associated with adult periodontitis. The adherence of the organism to host epithelium signals changes in both cell types as bacteria initiate infection and colonization and epithelial cells rally their defenses. We hypothesized that the expression of a defined set of P. gingivalis genes would be consistently up-regulated during infection of HEp-2 human epithelial cells. P. gingivalis genome microarrays were used to compare the gene expression profiles of bacteria that adhered to HEp-2 cells and bacteria that were incubated alone. Genes whose expression was temporally up-regulated included those involved in the oxidative stress response and those encoding heat shock proteins that are essential to maintaining cell viability under adverse conditions. The results suggest that contact with epithelial cells induces in P. gingivalis stress-responsive pathways that promote the survival of the bacterium.

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