scholarly journals Tuatara (Sphenodon) Genomics: BAC Library Construction, Sequence Survey, and Application to the DMRT Gene Family

2006 ◽  
Vol 97 (6) ◽  
pp. 541-548 ◽  
Author(s):  
Zhenshan Wang ◽  
Tsutomu Miyake ◽  
Scott V. Edwards ◽  
Chris T. Amemiya
Keyword(s):  
Gene Family ◽  
Bac Library ◽  
Construction Sequence ◽  
Library Construction

scholarly journals Nuclear genome size and karyotype analysis in Papaver for BAC library construction

2005 ◽  
Vol 21 (2) ◽  
pp. 145-150
Author(s):  
T. K. Kyrylenko ◽  
O. I. Martynenko ◽  
O. G. Alkhimova
Keyword(s):  
Genome Size ◽  
Karyotype Analysis ◽  
Bac Library ◽  
Nuclear Genome ◽  
Library Construction

scholarly journals Korean BAC Library Construction and Characterization of HLA-DRA, HLA-DRB3

BMB Reports ◽  
2006 ◽  
Vol 39 (4) ◽  
pp. 418-425 ◽  
Author(s):  
Mi-Hyun Park ◽  
Hye-Ja Lee ◽  
Jeong Bok ◽  
Cheol-Hwan Kim ◽  
Seong-Tshool Hong ◽  
...  
Keyword(s):  
Bac Library ◽  
Library Construction

scholarly journals BAC library construction and BAC end sequencing of five Drosophila species: the comparative map with the D. melanogaster genome

2008 ◽  
Vol 83 (3) ◽  
pp. 245-256 ◽  
Author(s):  
Katsuhiko Murakami ◽  
Atsushi Toyoda ◽  
Masahira Hattori ◽  
Yoko Kuroki ◽  
Asao Fujiyama ◽  
...  
Keyword(s):  
Bac Library ◽  
Drosophila Species ◽  
Library Construction ◽  
Comparative Map ◽  
Bac End Sequencing

Comprehensive DNA copy number profile and BAC library construction of an Indian individual

Gene ◽  
2012 ◽  
Vol 500 (2) ◽  
pp. 186-193 ◽  
Author(s):  
Sanjiban Chakrabarty ◽  
Reena R. D'Souza ◽  
Ravishankara Bellampalli ◽  
Harish Rotti ◽  
Abdul V. Saadi ◽  
...  
Keyword(s):  
Copy Number ◽  
Bac Library ◽  
Copy Number Profile ◽  
Dna Copy Number ◽  
Library Construction

The Pic19 NBS-LRR gene family members are closely linked to Scmv1, but not involved in maize resistance to sugarcane mosaic virus

Genome ◽  
2008 ◽  
Vol 51 (9) ◽  
pp. 673-684 ◽  
Author(s):  
Lu Jiang ◽  
Christina Rønn Ingvardsen ◽  
Thomas Lübberstedt ◽  
Mingliang Xu
Keyword(s):  
Gene Family ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Virus Disease ◽  
Family Members ◽  
Bac Library ◽  
Sugarcane Mosaic Virus ◽  
Isolation And Characterization ◽  
Maize Resistance ◽  
Bac Contigs

Sugarcane mosaic virus (SCMV) is the causal pathogen for a severe mosaic virus disease of maize worldwide. In our previous research, the maize resistance gene analog (RGA) Pic19 and its three cognate BAC contigs were mapped to the same region as the SCMV resistance gene Scmv1. Here we report the isolation and characterization of the Pic19R gene family members from the inbred line FAP1360A, which shows complete resistance to SCMV. Two primer pairs were designed based on the conserved regions among the known Pic19 paralogs and used for rapid amplification of cDNA ends of FAP1360A. Six full-length cDNAs, corresponding to the Pic19R-1 to -6 paralogs, were obtained. Three of them (Pic19R-1 to -3) had uninterrupted coding sequences and were, therefore, regarded as candidates for the Scmv1 gene. A total of 18 positive BAC clones harboring the Pic19R-2 to -5 paralogs were obtained from the FAP1360A BAC library and assembled into two BAC contigs. Two markers, tagging Pic19R-2 and -3 and Pic19R-4, were developed and used to genotype a high-resolution mapping population segregating solely for the Scmv1 locus. Although closely linked, none of these three Pic19R paralogs co-segregated with the Scmv1 locus. Analysis of the Pic19R family indicated that the Pic19R-1 paralog is identical to the known Rxo1 gene conferring resistance to rice bacterial streak disease and none of the other Pic19R paralogs seems to be involved in resistance to SCMV.

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