scholarly journals Noninvasive Genotyping and Mendelian Analysis of Microsatellites in African Savannah Elephants

2005 ◽  
Vol 96 (6) ◽  
pp. 679-687 ◽  
Author(s):  
J. B. A. Okello ◽  
G. Wittemyer ◽  
H. B. Rasmussen ◽  
I. Douglas-Hamilton ◽  
S. Nyakaana ◽  
...  
Keyword(s):  
Mendelian Analysis

GENETICS OF THE TUBULIN GENE FAMILIES OF PHYSARUM

Genetics ◽  
1984 ◽  
Vol 108 (1) ◽  
pp. 143-164
Author(s):  
Tim Schedl ◽  
Judi Owens ◽  
William F Dove ◽  
Timothy G Burland
Keyword(s):  
Drug Resistance ◽  
Genetic Mapping ◽  
Gene Family ◽  
Gene Families ◽  
Resistance Locus ◽  
Tubulin Gene ◽  
Length Polymorphisms ◽  
Mapping Analysis ◽  
Mendelian Analysis ◽  
Β Tubulin

ABSTRACT The organization of the α- and β-tubulin gene families in Physarum was investigated by Mendelian analysis. Restriction endonuclease-generated DNA fragments homologous to α- and β-tubulin show length polymorphisms that can be used as markers for genetic mapping. Analysis of meiotic assortment among progeny of heterozygotes allowed α- and β-tubulin sequence loci to be defined. There are four unlinked α-tubulin sequence loci (altA, altB, altC and altD) and at least three unlinked β-tubulin sequence loci (betA, betB and betC). The α-tubulin loci are not linked to the β-tubulin loci. —Segregation of tubulin sequence loci with respect to ben mutations that confer resistance to antitubulin benzimidazole drugs was used to investigate whether any members of the α- or β-tubulin gene families are allelic to ben loci. The β-tubulin sequence locus betB is allelic to the resistance locus benD, the betA locus is probably allelic to benA and the α-tubulin sequence locus altC may be allelic to benC. The molecular implications of benzimidazole resistance phenotypes when only one of the expressed β-tubulin gene family members mutates to drug resistance are discussed in relation to tubulin function.

scholarly journals Mating-type specific ribosomal proteins control aspects of sexual reproduction in Cryptococcus neoformans

2019 ◽  
Author(s):  
Giuseppe Ianiri ◽  
Yufeng “Francis” Fang ◽  
Tim A. Dahlmann ◽  
Shelly Applen Clancey ◽  
Guilhem Janbon ◽  
...  
Keyword(s):  
Sexual Reproduction ◽  
Cryptococcus Neoformans ◽  
Mating Type ◽  
Ribosomal Proteins ◽  
Gene Replacement ◽  
Mutant Strains ◽  
Targeted Gene Replacement ◽  
Mating Type Genes ◽  
Mendelian Analysis ◽  
Dependent Mechanism

AbstractThe MAT locus of Cryptococcus neoformans has a bipolar organization characterized by an unusually large structure, spanning over 100 kb. MAT genes have been characterized by functional genetics as being involved in sexual reproduction and virulence. However, classical gene replacement failed to achieve mutants for five MAT genes (RPL22, RPO41, MYO2, PRT1, RPL39), indicating that they are likely essential. In the present study, targeted gene replacement was performed in a diploid strain for both the α and a alleles of the ribosomal genes RPL22 and RPL39. Mendelian analysis of the progeny confirmed that both RPL22 and RPL39 are essential for viability. Ectopic integration of the RPL22 allele of opposite MAT identity in the heterozygous RPL22a/rpl22αΔ or RPL22α/rpl22aΔ mutant strains failed to complement their essential phenotype. Evidence suggests that this is due to differential expression of the RPL22 genes, and an RNAi-dependent mechanism that contributes to control RPL22a expression. Furthermore, via CRISPR/Cas9 technology the RPL22 alleles were exchanged in haploid MATα and MATa strains of C. neoformans. These RPL22 exchange strains displayed morphological and genetic defects during bilateral mating. These results contribute to elucidate functions of C. neoformans essential mating type genes that may constitute a type of imprinting system to promote inheritance of nuclei of both mating types.

scholarly journals Mating-Type-Specific Ribosomal Proteins Control Aspects of Sexual Reproduction in Cryptococcus neoformans

Genetics ◽  
2019 ◽  
Vol 214 (3) ◽  
pp. 635-649 ◽  
Author(s):  
Giuseppe Ianiri ◽  
Yufeng “Francis” Fang ◽  
Tim A. Dahlmann ◽  
Shelly Applen Clancey ◽  
Guilhem Janbon ◽  
...  
Keyword(s):  
Sexual Reproduction ◽  
Cryptococcus Neoformans ◽  
Mating Type ◽  
Ribosomal Proteins ◽  
Gene Replacement ◽  
Mutant Strains ◽  
Targeted Gene Replacement ◽  
Mating Type Genes ◽  
Mendelian Analysis ◽  
Dependent Mechanism

The MAT locus of Cryptococcus neoformans has a bipolar organization characterized by an unusually large structure, spanning over 100 kb. MAT genes have been characterized by functional genetics as being involved in sexual reproduction and virulence. However, classical gene replacement failed to achieve mutants for five MAT genes (RPL22, RPO41, MYO2, PRT1, and RPL39), indicating that they are likely essential. In the present study, targeted gene replacement was performed in a diploid strain for both the α and a alleles of the ribosomal genes RPL22 and RPL39. Mendelian analysis of the progeny confirmed that both RPL22 and RPL39 are essential for viability. Ectopic integration of the RPL22 allele of opposite MAT identity in the heterozygous RPL22a/rpl22αΔ or RPL22α/rpl22aΔ mutant strains failed to complement their essential phenotype. Evidence suggests that this is due to differential expression of the RPL22 genes, and an RNAi-dependent mechanism that contributes to control RPL22a expression. Furthermore, via CRISPR/Cas9 technology, the RPL22 alleles were exchanged in haploid MATα and MATa strains of C. neoformans. These RPL22 exchange strains displayed morphological and genetic defects during bilateral mating. These results contribute to elucidating functions of C. neoformans essential mating type genes that may constitute a type of imprinting system to promote inheritance of nuclei of both mating types.

Genetics of seed impermeability in soya bean

1987 ◽  
Vol 108 (2) ◽  
pp. 305-310 ◽  
Author(s):  
V. D. Verma ◽  
H. H. Ram
Keyword(s):  
Diallel Analysis ◽  
Graphical Analysis ◽  
Soya Bean ◽  
Maternal Control ◽  
Hard Seed ◽  
Black Seed ◽  
Normal Seed ◽  
Recessive Genes ◽  
Mendelian Analysis ◽  
Dominant Genes

SummarySix parental cultivars (Bragg, Bragg-black seed, Kalitur, Ankur, WT-125 and Glycine formosana), 15 F1's, eight F2's and eight F3's (Bragg × WT-125, Bragg × Glycine formosana, Bragg-black seed × WT 125, Bragg-black seed × Glycine formosana, Kalitur × WT-125, Kalitur × Glycine formosana, Ankur × WT-125 and Ankur × Glycine formosana) were evaluated for seed impermeability. WT-125 and Glycine formosana were identified as impermeable types (92·18, 77·46% impermeability, respectively). The other four varieties, namely Bragg (2·93% impermeable seeds), Bragg-black seed (11·7% impermeable seeds), Kalitur (no impermeable seeds) and Ankur (0·94% impermeable seeds) had soft seed or normal seed.The segregation pattern for impermeability in the F2 and F3 generations of eight crosses showed the involvement of a pair of dominant genes in Bragg × Glycine formosana, Bragg-black seed × WT-125, and Bragg-black seed × Glycine formosana, three pairs of dominant genes in Bragg × WT-125, Ankur × WT-125 and Ankur × Glycine formosana, and four pairs of dominant genes in Kalitur × WT-125 and Kalitur × Glycine formosana for seed impermeability, assuming maternal control as hard seed was a seed-coat phenomenon.Vr, Wr graphical analysis also indicated that WT-125 and Glycine formosana possessed mostly the dominant genes for seed impermeability, while the remaining four cultivars contained mostly the recessive genes. The correlation coefficient of Yr (parental mean) and Vr + Wr was negative and high (— 0·907) confirming that most of the dominant genes in the parental cultivars were acting towards high seed impermeability. Thus the overall results obtained through diallel analysis of 15 F1's were in agreement with those obtained through Mendelian analysis.

A genetic study on Gracilaria sjoestedtii

1988 ◽  
Vol 66 (10) ◽  
pp. 2022-2026 ◽  
Author(s):  
Xuecheng Zhang ◽  
John P. van der Meer
Keyword(s):  
Chromosome Number ◽  
Genetic Study ◽  
Maternal Inheritance ◽  
Intragenic Recombination ◽  
Wild Type ◽  
Multiple Alleles ◽  
Wild Type Clone ◽  
Nuclear Mutations ◽  
Diploid Gametes ◽  
Mendelian Analysis

In the present study on Gracilaria sjoestedtii, 41 mutants (40 pigmentation and 1 morphological) were isolated. These were characterized in a Mendelian analysis, which showed that 26 of the pigmentation variants are recessive nuclear mutations. The remaining 14 are cytoplasmic mutants having maternal inheritance and no detectable transmission through spermatia. A partial complementation analysis identified seven cistrons among the nuclear pigmentation mutants. Multiple alleles were encountered at five loci and apparent intragenic recombination was observed. No linkage between cistrons was found in recombination tests conducted thus far. Mitotic recombination leading to diploid gametes on tetrasporophytes was observed and used to construct triploid tetrasporophytes. The chromosome number of a wild-type clone of G. sjoestedtii was determined at meiosis as n = 29–30. Trivalent chromosomes were observed during meiosis in tetrasporophytes obtained by crossing female plants with tetrasporophytes. In culture tanks with flowing seawater, female gametophytes grew faster and appeared more suitable for vegetative propagation than male gametophytes and tetrasporophytes.

INHERITANCE OF RESISTANCE TO POWDERY MILDEW IN THE CULTIVATED STRAWBERRY

1969 ◽  
Vol 11 (2) ◽  
pp. 426-438 ◽  
Author(s):  
C. S. Hsu ◽  
R. Watkins ◽  
A. T. Bolton ◽  
L. P. S. Spangelo
Keyword(s):  
Powdery Mildew ◽  
Quantitative Method ◽  
Powdery Mildew Resistance ◽  
Inheritance Of Resistance ◽  
Complementary Information ◽  
Additive Variance ◽  
Discontinuous Variation ◽  
Mendelian Analysis ◽  
Strawberry Cultivars ◽  
Additive Genes

The inheritance of resistance to powdery mildew was investigated in 64-somewhat interdependent progenies produced among 32 North American strawberry cultivars or selections. The data were analyzed by both the quantitative method based on continuous variation and the Mendelian method based on discontinuous variation. The quantitative analysis indicated that non-additive variance was more important than additive variance and that epistatic variance was considerable. The Mendelian analysis supported the hypothesis that segregation depended on two additive genes for resistance and one epistatic gene far susceptibility. The breeding procedure of progeny selection followed by individual selection was suggested for the improvement of powdery mildew resistance in strawberry based on the complementary information obtained from both analyses.

scholarly journals The Importance of Automation in Genetic Diagnosis: Lessons from Analyzing an Inherited Retinal Degeneration Cohort with the Mendelian Analysis Toolkit (MATK)

2021 ◽  
Author(s):  
Erin Zampaglione ◽  
Matthew Maher ◽  
Emily M. Place ◽  
Naomi E. Wagner ◽  
Stephanie DiTroia ◽  
...  
Keyword(s):  
Retinal Degeneration ◽  
Human Error ◽  
Genetic Diagnosis ◽  
Disease Diagnosis ◽  
Specific Gene ◽  
Mendelian Disease ◽  
Variant Information ◽  
Assisted Analysis ◽  
Inherited Retinal Degeneration ◽  
Mendelian Analysis

Purpose: In Mendelian disease diagnosis, variant analysis is a repetitive, error-prone, and time-consuming process. To address this, we have developed the Mendelian Analysis Toolkit (MATK), a configurable automated variant ranking program. Methods: MATK aggregates variant information from multiple annotation sources and uses expert-designed rules with parameterized weights to produce a ranked list of potentially causal solutions. MATK performance was measured by a comparison of MATK-aided versus human domain-expert analyses of 1060 inherited retinal degeneration (IRD) families investigated with an IRD-specific gene panel (589 families) and exome sequencing (471 families). Results: When comparing MATK-assisted analysis to expert curation, we found that 97.3% (541/556) of potential solutions found by experts were also identified by the MATK-assisted analysis. Furthermore, MATK-assisted analysis identified 114 additional potential solutions. The software also showed utility in data reanalysis after remapping to the GRCh38 genome build. Conclusion: MATK expedites the process of identifying likely solving variants in Mendelian traits and helps to remove variability coming from human error and researcher bias. MATK facilitates data re-analysis to keep up with the constantly improving annotation sources and NGS processing pipelines. The software is open source and available at https://gitlab.partners.org/meei-ogi-bioinformatics/MendelAnalysis

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