Impact of Temperature Storage Conditions of Halyomorpha halys (Hemiptera: Pentatomidae) Eggs on Parasitism by Anastatus reduvii (Hymenoptera: Eupelmidae)

2019 ◽  
Author(s):  
Emily C Ogburn ◽  
James F Walgenbach
Keyword(s):  
Storage Conditions ◽  
Halyomorpha Halys ◽  
Host Feeding ◽  
Stink Bug ◽  
Eastern United States ◽  
Stink Bugs ◽  
Different Temperatures ◽  
Anastatus Reduvii ◽  
The Impact ◽  
And Storage

Abstract Brown marmorated stink bug, Halyomorpha halys Stål, is an invasive species of Asian origin that is an important agricultural pest in the eastern United States. Sentinel egg masses are tools used to assess the impact of natural enemies on H. halys populations. To determine the effect of host egg age and storage conditions on their susceptibility to parasitism, H. halys eggs were stored at different temperatures for different lengths of time and then exposed to Anastatus reduvii (Howard), a native natural enemy of H. halys occurring in eastern North America. For eggs stored at 15, 20, and 25°C and then exposed to A. reduvii, the number of host eggs from which parasitoid offspring emerged declined with age of eggs. Control eggs (exposed to parasitoids without being stored) and those eggs stored for only 5.5 degree-days (DD) (=0.5 days) at 25°C yielded the highest percentage of parasitoids at 88.2 and 88.3%, respectively. For eggs stored at 20 and 25°C for 7.3 DD to about 36 DD, offspring emerged from about 58 to 73% of eggs, and total parasitism (emerged + unemerged parasitoids) ranged from about 70 to 80%. Parasitoid emergence was significantly lower for host eggs stored at 15°C for comparable times at 20 and 25°C. Stink bugs nymphs hatched from <0.6% of all eggs. Parasitoid-induced host egg abortion was an important component of egg mortality caused by A. reduvii, with underdeveloped stink bug nymphs, undifferentiated cell contents, and parasitoid host feeding occurring across all storage treatments.

scholarly journals Changes of Physicochemical Properties of Starch Syrups Recommended for Winter Feeding of Honeybees during Storage

Agriculture ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 374
Author(s):  
Teresa Szczęsna ◽  
Ewa Waś ◽  
Piotr Semkiw ◽  
Piotr Skubida ◽  
Katarzyna Jaśkiewicz ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Physicochemical Properties ◽  
Physicochemical Parameters ◽  
Storage Temperature ◽  
Storage Conditions ◽  
Different Temperatures ◽  
And Storage ◽  
Bee Colonies ◽  
Temperature Time ◽  
Winter Feeding

The aim of this study was to determine the influence of storage temperature and time on physicochemical parameters of starch syrups recommended for the winter feeding of bee colonies. The studies included commercially available three starch syrups and an inverted saccharose syrup that were stored at different temperatures: ca. 20 °C, 10–14 °C, and ca. 4 °C. Physicochemical parameters of fresh syrups (immediately after purchase) and syrups after 3, 6, 9, 12, 15, 18, 21, and 24 months of storage at the abovementioned temperatures were measured. It was observed that the rate of unfavorable changes in chemical composition of starch syrups and the inverted saccharose syrup, mainly the changes in the 5-hydroxymethylfurfural (HMF) content, depended on the type of a syrup and storage conditions (temperature, time). Properties of tested starch syrups intended for winter feeding of bees stored at ca. 20 °C maintained unchanged for up to 6 months, whereas the same syrups stored at lower temperatures (10–14 °C) maintained unchanged physicochemical parameters for about 12 months. In higher temperatures, the HMF content increased. To date, the influence of this compound on bees has not been thoroughly investigated.

Baseline Toxicity of the Insecticides Bifenthrin and Thiamethoxam on Halyomorpha halys (Hemiptera: Pentatomidae) Collected From the Eastern United States

2020 ◽  
Vol 113 (2) ◽  
pp. 1043-1046
Author(s):  
Adam Alford ◽  
Thomas P Kuhar ◽  
George C Hamilton ◽  
Peter Jentsch ◽  
Grzgorz Krawczyk ◽  
...  
Keyword(s):  
United States ◽  
Ornamental Plants ◽  
The United States ◽  
Halyomorpha Halys ◽  
Brown Marmorated Stink Bug ◽  
Stink Bug ◽  
Eastern United States ◽  
Agronomic Crops ◽  
Baseline Levels ◽  
Topical Applications

Abstract Brown marmorated stink bug, Halyomorpha halys (Stål), is an invasive species in the United States that attacks a wide variety of agricultural commodities including fruits, vegetables, agronomic crops, and ornamental plants. Populations of H. halys adults were collected from four and six states in 2017 and 2018, respectively, and tested using topical applications to establish baseline levels of susceptibility to two commonly used insecticides, bifenthrin and thiamethoxam. A Probit-estimated (95% fiducial limits) LD50 and LD99 of 2.64 g AI/L (1.2–3.84 g AI/L) and 84.96 g AI/L (35.76–716.16 g AI/L) for bifenthrin, and a LD50 and LD99 of 0.05 g AI/liter (1.14E-5–0.27 g AI/L) and 150.11 g AI/L (27.35–761,867 g AI/L) for thiamethoxam, respectively. These baseline levels can be used for future insecticide resistance monitoring in H. halys.

Effect of Trap Color and Residual Attraction of a Pheromone Lure for Monitoring Stink Bugs (Hemiptera: Pentatomidae)

2020 ◽  
Vol 55 (4) ◽  
pp. 437-447
Author(s):  
Ted E. Cottrell ◽  
Rammohan R. Balusu ◽  
Edgar Vinson ◽  
Bryan Wilkins ◽  
Henry Y. Fadamiro ◽  
...  
Keyword(s):  
Native Species ◽  
Halyomorpha Halys ◽  
Stink Bug ◽  
Stink Bugs ◽  
Euschistus Servus ◽  
Trap Color ◽  
Residual Efficacy ◽  
Conducting Research ◽  
Multiple Species ◽  
Research Studies

Abstract Stink bugs (Hemiptera: Pentatomidae) are commonly monitored using pyramid traps baited with a pheromone. Initially, the pyramid traps were painted yellow and predominantly used to monitor native stink bug species. However, research studies involving the exotic Halyomorpha halys Stål (Hemiptera: Pentatomidae) now use pyramid traps that are black, not yellow. As H. halys moves across the southeastern United States, the use of a single trap, yellow or black, for monitoring and conducting research studies would be beneficial. Our objective was to compare black and yellow pyramid traps baited with a lure to determine if one was superior for trapping herbivorous stink bugs. This study was conducted at four locations, three in Alabama and one in Georgia, over 2 yr. Additionally, residual efficacy of the lure was measured via trap capture over 1-mo intervals. Our results showed that only when native stink bug species were combined, and only in 1 yr, were captures significantly affected by trap color. Capture of the exotic H. halys and the most abundant native species, Euschistus servus (Say), was not significantly affected by trap color. Trap capture was significantly affected by how long a lure was in a trap. The data from this study suggests that when traps are used in conjunction with a pheromone to monitor multiple species of adult stink bugs, especially native species, the yellow pyramid trap is favored.

Damage to Soybean by Acrosternum hilare (Say) (Heteroptera: Pentatomidae)

2008 ◽  
Vol 43 (3) ◽  
pp. 257-267 ◽  
Author(s):  
S. Y. Young ◽  
J. K. Greene ◽  
G. M. Lorenz
Keyword(s):  
Yield Loss ◽  
Stink Bug ◽  
Stink Bugs ◽  
Field Cage ◽  
Acrosternum Hilare ◽  
Seed Damage ◽  
Crop Development ◽  
Green Stink Bug ◽  
Significant Yield ◽  
The Impact

A series of field-cage experiments were conducted in 2002 and 2003 in southeast Arkansas to measure the impact of feeding by green stink bug, Acrosternum hilare (Say), on soybean yield and seed damage. Stink bugs were collected from local soybean fields and released in 1.8 × 1.8 × 1.8 m walk-in screen cages at densities of 0, 3, 9, and 18 bugs per row-m on maturity group (MG) IV and MG V soybean at different stages of crop phenological development. Damaged seed at harvest was significantly related to caged density of A. hilare in most of the cage experiments. Yield loss was associated with density of caged bugs in 7 of 11 different experiments and ranged from 13.4–60.5 kg/ha (0.2–0.9 bu/a) lost per bug per row-m. MG IV and MG V soybean were similarly impacted by density of A. hilare when exposed at the same stage of crop development. Feeding by small and large nymphs at early (R2–R3) and mid (R5–R6) reproductive stages resulted in significant yield loss. Feeding damage was apparent on late reproductive stage soybean (R7–R8), but no measurable impact on yield was observed. This suggested that thresholds could be raised or control efforts could be terminated for stink bugs infesting R7–R8 stage soybean. Damage due to feeding by stink bugs is related to various factors, but crop phenology, density of bugs, and length of infestation time are consistent and predictable influences that are interrelated, and all should be considered in determining the need to control field populations.

scholarly journals Encapsulation of Lactobacillus acidophilus La-5 and Bifidobacterium Bb-12 by spray drying and evaluation of its resistance in simulated gastrointestinal conditions, thermal treatments and storage conditions

2018 ◽  
Vol 48 (6) ◽  
Author(s):  
Graciele Lorenzoni Nunes ◽  
Mariana Heldt Motta ◽  
Alexandre José Cichoski ◽  
Roger Wagner ◽  
Édson Irineu Muller ◽  
...  
Keyword(s):  
Thermal Resistance ◽  
Lactobacillus Acidophilus ◽  
Encapsulation Efficiency ◽  
Storage Conditions ◽  
Thermal Treatments ◽  
Spray Dryer ◽  
Gastrointestinal Conditions ◽  
Different Temperatures ◽  
And Storage ◽  
Gastrointestinal Simulation

ABSTRACT: Lactobacillus acidophillus La-5 (ML) and Bifidobacterium Bb-12 (MB) microparticles were produced at different temperatures by spray dryer. The influence of different temperatures on the viability, encapsulation efficiency, water activity and moisture were evaluated. Microparticles that presented more viability were submitted to thermal resistance, gastrointestinal simulation, storage stability, morphology and particle size analyses. Drying temperature of 130°C showed higher encapsulation efficiency, 84.61 and 79.73% for Lactobacillus acidophillus (ML) and Bifidobacterium Bb-12 (MB) microparticles, respectively. In the evaluation of thermal resistance and gastrointestinal simulation, the microparticles of Lactobacillus acidophillus La-5 (ML) presented higher survival than Bifidobacterium Bb-12 (MB) under these conditions. In storage viability only the Lactobacillus acidophillus La-5 (ML) microparticles remained viable at all evaluated temperatures during the 120 days. The particle sizes reported were 4.85 for Lactobacillus acidophillus La-5 (ML) and 8.75 for Bifidobacterium Bb-12 (MB), being in agreement with the desired values for products obtained by spray dryer. Finally, the Lactobacillus acidophilus La-5 (ML) microparticles were shown to be more resistant under the conditions evaluated in this study.

scholarly journals Biosecurity risks from stink bugs to New Zealand kiwifruit identified in Chinese language literature

2018 ◽  
Vol 71 ◽  
pp. 140-150 ◽  
Author(s):  
David A.J. Teulon ◽  
Bingqin Xu
Keyword(s):  
New Zealand ◽  
English Language ◽  
Chinese Characters ◽  
Stink Bug ◽  
Stink Bugs ◽  
Knowledge Infrastructure ◽  
Literature Searches ◽  
The Impact ◽  
Chinese National Knowledge Infrastructure ◽  
Language Literature

Brown marmorated stink bug (BMSB), native to Asia including China, is a major invasive horticultural and crop pest in North America and Europe, and now threatens the southern hemisphere. BMSB has not established in New Zealand although it is regularly intercepted at its borders. Relatively little is known about the impact of BMSB on kiwifruit, an important horticultural crop in New Zealand; at least in English language literature. Searches were conducted in the Chinese National Knowledge Infrastructure (CNKI) platform using Chinese characters for BMSB (茶翅蝽) and kiwifruit (猕猴桃), and also in English, in international platforms. We identified 17 and eight publications, respectively, indicating that BMSB and yellow spotted stinkbug (YSSB) (麻皮蝽 and 黄斑蝽) are major pests of kiwifruit in China. Little information on BMSB or YSSB and their pest status in kiwifruit was found in English language searches in international platforms. Searching Chinese databases with Chinese characters in combination with searches in international databases is necessary to ensure comprehensive coverage for biosecurity risk assessment.

scholarly journals Expression Profiles and Functional Characterization of Chemosensory Protein 15 (HhalCSP15) in the Brown Marmorated Stink Bug Halyomorpha halys

2021 ◽  
Vol 12 ◽  
Author(s):  
Zehua Wang ◽  
Fan Yang ◽  
Ang Sun ◽  
Shuang Shan ◽  
Yongjun Zhang ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Functional Characterization ◽  
Insect Species ◽  
Pcr Analysis ◽  
Halyomorpha Halys ◽  
Stink Bug ◽  
Stink Bugs ◽  
Binding Assays ◽  
Chemosensory Protein ◽  
Reverse Transcription Pcr

Chemosensory proteins (CSPs) have been identified in the sensory tissues of various insect species and are believed to be involved in chemical communication in insects. However, the physiological roles of CSPs in Halyomorpha halys, a highly invasive insect species, are rarely reported. Here, we focused on one of the antennal CSPs (HhalCSP15) and determined whether it was involved in olfactory perception. Reverse transcription PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR) analysis showed that HhalCSP15 was enriched in nymph and male and female adult antennae, indicating its possible involvement in the chemosensory process. Fluorescence competitive binding assays revealed that three of 43 natural compounds showed binding abilities with HhalCSP15, including β-ionone (Ki=11.9±0.6μM), cis-3-hexen-1-yl benzoate (Ki=10.5±0.4μM), and methyl (2E,4E,6Z)-decatrienoate (EEZ-MDT; Ki=9.6±0.8μM). Docking analysis supported the experimental affinity for the three ligands. Additionally, the electrophysiological activities of the three ligands were further confirmed using electroantennography (EAG). EEZ-MDT is particularly interesting, as it serves as a kairomone when H. halys forages for host plants. We therefore conclude that HhalCSP15 might be involved in the detection of host-related volatiles. Our data provide a basis for further investigation of the physiological roles of CSPs in H. halys, and extend the olfactory function of CSPs in stink bugs.

scholarly journals The influence of geographical origin on honey composition studied by Polish and Slovak honeys

2019 ◽  
Vol 37 (No. 4) ◽  
pp. 232-238 ◽  
Author(s):  
Monika Tomczyk ◽  
Maria Tarapatskyy ◽  
Małgorzata Dżugan
Keyword(s):  
Geographical Origin ◽  
Total Phenolics ◽  
Storage Conditions ◽  
Botanical Origin ◽  
Colour Intensity ◽  
Statistical Tool ◽  
Sugar Profile ◽  
The Impact ◽  
And Storage

Honey composition is mainly affected by botanical origin, however geographical factors as well as beekeeping practice and storage conditions can also influence its quality. The aim of the study was to determine the impact of geographical origin on physicochemical quality and biological activity of honey. For this reason Polish and Slovak varietal honeys, including per each country: 10 multifloral, 5 tilia, 5 rape, 5 acacia and 5 forest were compared according to their physicochemical parameters (free acidity, pH, electrical conductivity, moisture content, and colour intensity), sugar profile, diastase activity, as well as antioxidant activity (DPPH and FRAP tests, as well as photochemiluminescence method). Moreover, total phenolics compounds and flavonoids content were determined. The most significant differences (P &lt; 0.05) between Polish and Slovak counterparts were found for tilia while the lowest for rape honeys. The impact of geographical origin on overall quality of honey was proved by PCA statistical tool.

scholarly journals Preservation of Azotobacter chroococcum vegetative cells in dry polymers

2014 ◽  
Vol 20 (2) ◽  
pp. 201 ◽  
Author(s):  
Daniel Rojas-Tapias ◽  
Oriana Ortega Sierra ◽  
Diego Rivera Botía ◽  
Ruth Bonilla
Keyword(s):  
Storage Conditions ◽  
Azotobacter Chroococcum ◽  
Bacterial Degradation ◽  
Protective Agent ◽  
Degradation Rates ◽  
Different Temperatures ◽  
And Storage ◽  
Temperature Time ◽  
Long Term Preservation

We studied the preservation of Azotobacter chroococcum C26 using three dry polymers: carrageenin, sodium alginate, and HPMC, using a method of accelerated degradation. Bacterial viability, as response variable, was measured at three temperatures in four different times, which was followed by calculation of bacterial degradation rates. Results showed that temperature, time of storage, and protective agent influenced both viability and degradation rates (P;lt;0.05). We observed, using the Arrhenius thermodynamic model, that the use of polymers increased the activation energy of bacterial degradation compared to control. We obtained thermodynamic models for each polymer, based on the Arrhenius equation, which predicted the required time for thermal degradation of the cells at different temperatures. Analysis of the models showed that carrageenin was the best polymer to preserve A. chroococcum C26 since ~ 900 days are required at 4 ºC to reduce its viability in two log units. We conclude, therefore, that long-term preservation of A. chroococcum C26 using dry polymers is suitable under adequate preservation and storage conditions.

scholarly journals Lethal and sub-lethal effects of low-temperature exposures on Halyomorpha halys (Hemiptera: Pentatomidae) adults before and after overwintering

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Davide Scaccini ◽  
Luka Vanishvili ◽  
Paola Tirello ◽  
Vaughn M. Walton ◽  
Carlo Duso ◽  
...  
Keyword(s):  
Low Temperature ◽  
Cold Exposure ◽  
Mortality Rates ◽  
Adult Survival ◽  
Adult Longevity ◽  
Halyomorpha Halys ◽  
Stink Bug ◽  
Spring Frost ◽  
Nutrient Index ◽  
The Impact

Abstract Cold winter temperatures can influence insects’ survival in temperate zones. Brown marmorated stink bug, Halyomorpha halys (Stål) overwinters as adults in natural and human-made structures. In this study, we characterized low temperature mortality rates of H. halys adults that were either entering (ENA) or exiting (EXA) overwintering microhabitats. We considered the effect of different duration of cold exposure on mortality. We determined the impact of insect nutritional status and weight on cold tolerance. We additionally evaluated the effects of cold exposure on H. halys adult longevity and fecundity. Mortality of ENA and EXA adults was determined for 6 h and 2 h periods at − 2.5 °C and 2.5 °C respectively. EXA adults displayed higher mortality rates compared to ENA individuals at the low-temperature regimes. Halyomorpha halys adult survival rate was higher when their nutrient index (EXA individuals only) and weight were high. Low-temperature exposure increased longevity, but reduced fecundity of ENA females. The data further highlight how extreme spring frost events can result in significantly increased mortality levels of H. halys adults.

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