Identification ofSirex noctilio(Hymenoptera: Siricidae) Using a Species-Specific Cytochrome C Oxidase Subunit I PCR Assay

2016 ◽  
Vol 109 (3) ◽  
pp. 1424-1430 ◽  
Author(s):  
Xueting Sun ◽  
Jing Tao ◽  
Lili Ren ◽  
Juan Shi ◽  
Youqing Luo
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Pcr Assay ◽  
Oxidase Subunit ◽  
Species Specific

scholarly journals New Species-Specific Primers for Molecular Diagnosis of Bactrocera minax and Bactrocera tsuneonis (Diptera: Tephritidae) in China Based on DNA Barcodes

Insects ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 447 ◽  
Author(s):  
Linyu Zheng ◽  
Yue Zhang ◽  
Wenzhao Yang ◽  
Yiying Zeng ◽  
Fan Jiang ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Dna Barcode ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Specific Primers ◽  
Oxidase Subunit ◽  
Preimaginal Stages ◽  
Specific Fragment ◽  
Species Specific

Tephritidae fruit flies (Diptera: Tephritidae) are regarded as important damage-causing species due to their ability to cause great economic losses in fruit and vegetable crops. Bactrocera minax and Bactrocera tsuneonis are two sibling species of the subgenus Tetradacus of Bactrocera that are distributed across a limited area of China, but have caused serious impacts. They share similar morphological characteristics. These characteristics can only be observed in the female adult individuals. The differences between them cannot be observed in preimaginal stages. Thus, it is difficult to distinguish them in preimaginal stages morphologically. In this study, we used molecular diagnostic methods based on cytochrome c oxidase subunit I and species-specific markers to identify these two species and improve upon the false-positive results of previous species-detection primers. DNA barcode sequences were obtained from 900 individuals of B. minax and 63 individuals of B. tsuneonis. Based on these 658 bp DNA barcode sequences of the cytochrome c oxidase subunit I gene, we successfully designed the species-specific primers for B. minax and B. tsuneonis. The size of the B. minax specific fragment was 422 bp and the size of the B. tsuneonis specific fragment was 456 bp. A series of PCR trials ensured the specificity of these two pairs of primers. Sensitivity assay results demonstrated that the detection limit for the DNA template concentration was 0.1~1 ng/μL for these two species. In this study, we established a more reliable, rapid, and low-cost molecular identification method for all life stages of B. minax and B. tsuneonis. Species-specific PCR can be applied in plant quarantine, monitoring and control of B. minax and B. tsuneonis.

Agriotes proximus and A. lineatus (Coleoptera: Elateridae): a comparative study on the pheromone composition and cytochrome c oxidase subunit I gene sequence

Chemoecology ◽  
2011 ◽  
Vol 22 (1) ◽  
pp. 23-28 ◽  
Author(s):  
József Vuts ◽  
Till Tolasch ◽  
Lorenzo Furlan ◽  
Éva Bálintné Csonka ◽  
Tamás Felföldi ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Comparative Study ◽  
Cytochrome C Oxidase ◽  
Gene Sequence ◽  
Oxidase Subunit ◽  
Pheromone Composition ◽  
I Gene

scholarly journals Structural organization of the rat cytochrome c oxidase subunit IV gene.

1990 ◽  
Vol 265 (13) ◽  
pp. 7687-7692
Author(s):  
M Yamada ◽  
N Amuro ◽  
Y Goto ◽  
T Okazaki
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Structural Organization ◽  
Oxidase Subunit
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