scholarly journals Demography and Population Projection of Flea Beetle,Agasicles hygrophila(Coleoptera: Chrysomelidae), Fed on Alligator Weed Under Elevated CO2

2016 ◽  
Vol 109 (3) ◽  
pp. 1116-1124 ◽  
Author(s):  
Jian-Wei Fu ◽  
Meng-Zhu Shi ◽  
Ting Wang ◽  
Jian-Yu Li ◽  
Li-Zhen Zheng ◽  
...  
Keyword(s):  
Elevated Co2 ◽  
Flea Beetle ◽  
Population Projection ◽  
Alligator Weed

Biotic stress of predators on population of alligator weed flea beetle,Agasicles hygrophila(Col.：Chrysomelidae)

2014 ◽  
Vol 34 (3) ◽  
Author(s):  
刘雨芳 LIU Yufang ◽  
李菲 LI Fei ◽  
桂芳艳 GUI Fangyan ◽  
王秀秀 WANG Xiuxiu ◽  
刘文海 LIU Wenhai ◽  
...  
Keyword(s):  
Biotic Stress ◽  
Flea Beetle ◽  
Alligator Weed

Response of the alligator weed flea beetle,Agasicles hygrophila(Coleoptera: Chrysomelidae) to overwintering protection and its controlling effect on alligator weedAlternanthera philoxeroides(Amaranthacese：Alternanthera)

2014 ◽  
Vol 34 (10) ◽  
Author(s):  
刘雨芳 LIU Yufang ◽  
王秀秀 WANG Xiuxiu ◽  
李菲 LI Fei ◽  
桂芳艳 GUI Fangyan ◽  
刘文海 LIU Wenhai ◽  
...  
Keyword(s):  
Flea Beetle ◽  
Alligator Weed ◽  
Controlling Effect

scholarly journals Identification and Evaluation of Reference Genes for Quantitative PCR Normalization in Alligator Weed Flea Beetle (Coleoptera: Chrysomelidae)

2021 ◽  
Vol 21 (5) ◽  
Author(s):  
Yan-Qiong Guo ◽  
Yongchang Yang ◽  
Yanping Chai ◽  
Ling-Ling Gao ◽  
Ruiyan Ma
Keyword(s):  
Ribosomal Protein ◽  
Gene Transcription ◽  
Reference Genes ◽  
Flea Beetle ◽  
Elongation Factor ◽  
Housekeeping Genes ◽  
Body Parts ◽  
Expression Stability ◽  
Alligator Weed ◽  
Experimental Conditions

Abstract Stably expressed reference genes are critical internal standards for the quantification of gene transcription levels using quantitative real-time PCR. Housekeeping genes are commonly used as reference genes but their expressions were variable depending on experimental conditions in many insect species studied. Here we report the identification and evaluation of 10 housekeeping genes in alligator weed flea beetle, Agasicles hygrophila Selman & Vogt (Coleoptera: Chrysomelidae), a biocontrol agent of alligator weed. The 10 housekeeping genes are: beta-actin (Actin), ribosomal protein L13A (PRL13a), succinate dehydrogenase complex subunit A (SDHA), ribosomal protein S20 (RPS20), ribosomal protein S13 (RPS13), glyceraldehyde phosphate dehydrogenase (GAPDH), TATA-box-binding protein (TBP), ribosomal protein L32 (RPL32), tubulin alpha-1 chain (TUBULIN), and elongation factor-1 alpha (ELF). Five programs, geNorm, NormFinder, BestKeeper, ΔCt method, and RefFinder, were used to evaluate the expression stability of the 10 genes among various A. hygrophila body parts and with different nutrient types (starvation, diet types). The expression stability analysis showed that RPS32 and RPL13a were reliable reference genes for the study of gene transcription in different body parts; Actin and RPL13a were optimal reference genes for different nutrient types. The selections of reference genes were validated using a CarE gene (GeneBank No: KX353552). The results of this study provide useful bases for studies of gene expression in various aspects relating to A. hygrophila.

Herbicides compromise biological control of ragwort

2002 ◽  
pp. 147-152
Author(s):  
K. Betteridge ◽  
D. Costall
Keyword(s):  
Biological Control ◽  
Pyrrolizidine Alkaloids ◽  
Animal Health ◽  
Flea Beetle ◽  
Dairy Farm ◽  
Senecio Jacobaea ◽  
Longitarsus Jacobaeae ◽  
High Ester ◽  
Low Levels

In spite of ragwort flea beetle (RFB) being present on a Dannevirke dairy farm, pastures were sprayed each winter to reduce ragwort density and limit the risk of ragwort poisoning of stock. The trial on this farm from June 1999 - October 2001, aimed to determine whether herbicide (H) impacted on RFB and how H and RFB each impacted on ragwort growth and persistence. RFBfree areas were created by spraying with insecticide (I). Effects of ragwort on animal health are also reported. High ester 2,4-D (H) boom-sprayed once only, in June 1999, killed most ragwort plants and reduced RFB larvae densities to low levels before the plants died. Once new ragwort established in treatment H, the plants became infested with RFB larvae. RFB larvae were suppressed by I resulting in ragwort density declining more slowly than in treatments where RFB were not suppressed. Insecticide treatments were stopped after 15 months and, at 24 months, ragwort could not be found within the trial area. Ragwort control was attributed to the cessation of herbicide spraying allowing the RFB population to reach a sufficient density to kill both small and large ragwort plants. Sub-clinical ragwort poisoning was found in livers of culled cows that had grazed on ragwort-dense pastures. Keywords: animal health, biological control, Longitarsus jacobaeae, pyrrolizidine alkaloids, ragwort, ragwort flea beetle, Senecio jacobaea

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