Capsid Gene Divergence of Black Queen Cell Virus Isolates in Thailand and Japan Honey Bee Species

2015 ◽  
Vol 108 (3) ◽  
pp. 1460-1464 ◽  
Author(s):  
W. Mookhploy ◽  
K. Kimura ◽  
T. Disayathanoowat ◽  
M. Yoshiyama ◽  
K. Hondo ◽  
...  
Keyword(s):  
Honey Bee ◽  
Capsid Gene ◽  
Black Queen Cell Virus ◽  
Gene Divergence ◽  
Queen Cell ◽  
Virus Isolates

scholarly journals Host range expansion of honey bee Black Queen Cell Virus in the bumble bee, Bombus huntii

Apidologie ◽  
2011 ◽  
Vol 42 (5) ◽  
pp. 650-658 ◽  
Author(s):  
Wenjun Peng ◽  
Jilian Li ◽  
Humberto Boncristiani ◽  
James P. Strange ◽  
Michele Hamilton ◽  
...  
Keyword(s):  
Host Range ◽  
Honey Bee ◽  
Range Expansion ◽  
Bumble Bee ◽  
Black Queen Cell Virus ◽  
Host Range Expansion ◽  
Queen Cell

scholarly journals The Pathogen Profile of a Honey Bee Queen Does Not Reflect That of Her Workers

Insects ◽  
2020 ◽  
Vol 11 (6) ◽  
pp. 382 ◽  
Author(s):  
Jessica L. Kevill ◽  
Katie Lee ◽  
Michael Goblirsch ◽  
Erin McDermott ◽  
David R. Tarpy ◽  
...  
Keyword(s):  
Honey Bee ◽  
Horizontal Transmission ◽  
Pathogen Transmission ◽  
Black Queen Cell Virus ◽  
Deformed Wing Virus ◽  
Israeli Acute Paralysis Virus ◽  
Queen Cell ◽  
Honey Bee Queen ◽  
Acute Bee Paralysis Virus ◽  
Paralysis Virus

Throughout a honey bee queen’s lifetime, she is tended to by her worker daughters, who feed and groom her. Such interactions provide possible horizontal transmission routes for pathogens from the workers to the queen, and as such a queen’s pathogen profile may be representative of the workers within a colony. To explore this further, we investigated known honey bee pathogen co-occurrence, as well as pathogen transmission from workers to queens. Queens from 42 colonies were removed from their source hives and exchanged into a second, unrelated foster colony. Worker samples were taken from the source colony on the day of queen exchange and the queens were collected 24 days after introduction. All samples were screened for Nosema spp., Trypanosome spp., acute bee paralysis virus (ABPV), black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), Israeli acute paralysis virus (IAPV), Lake Sinai virus (LSV), and deformed wing virus master variants (DWV-A, B, and C) using RT-qPCR. The data show that LSV, Nosema, and DWV-B were the most abundant pathogens in colonies. All workers (n = 42) were LSV-positive, 88% were Nosema-positive, whilst pathogen loads were low (<1 × 106 genome equivalents per pooled worker sample). All queens (n = 39) were negative for both LSV and Nosema. We found no evidence of DWV transmission occurring from worker to queen when comparing queens to foster colonies, despite DWV being present in both queens and workers. Honey bee pathogen presence and diversity in queens cannot be revealed from screening workers, nor were pathogens successfully transmitted to the queen.

scholarly journals An Investigation of Honey Bee Viruses Prevalence in Managed Honey Bees (Apis mellifera and Apis cerana) Undergone Colony Decline

2021 ◽  
Vol 15 (1) ◽  
pp. 58-66
Author(s):  
Chunying Yuan ◽  
Xuejian Jiang ◽  
Man Liu ◽  
Sa Yang ◽  
Shuai Deng ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Clinical Symptoms ◽  
Apis Cerana ◽  
Rt Pcr ◽  
Black Queen Cell Virus ◽  
Queen Cell ◽  
Bee Virus ◽  
Paralysis Virus ◽  
Bee Viruses

Objective: In the absence of known clinical symptoms, viruses were considered to be the most probable key pathogens of honey bee. Therefore, the aim of this study was to investigate the prevalence and distribution of honey bee viruses in managed Apis mellifera and Apis cerana in China. Methods: We conducted a screening of 8 honey bee viruses on A. mellifera and A. cerana samples collected from 54 apiaries from 13 provinces in China using RT-PCR. Results: We found that the types and numbers of viral species significantly differed between A. mellifera and A. cerana. Black Queen Cell Virus (BQCV), Chronic Bee Paralysis Virus (CBPV), Apis mellifera filamentous virus (AmFV), and Kakugo virus (DWV-A/KV) were the primary viruses found in A. mellifera colonies, whereas Chinese Sacbrood Bee Virus (CSBV) and Sacbrood Bee Virus (SBV) were the primary viruses found in A. cerana. The percentage infection of BQCV and CSBV were 84.6% and 61.6% in all detected samples. We first detected the occurrences of Varroa destructor virus-1 (VDV-1 or DWV-B) and DWV-A/KV in China but not ABPV in both A. mellifera and A. cerana. Conclusion: This study showed that BQCV and CSBV are the major threat to investigated A. mellifera and A. cerana colonies.

Occurrence, detection, and quantification of economically important viruses in healthy and unhealthy honey bee (Hymenoptera: Apidae) colonies in Canada

2015 ◽  
Vol 148 (1) ◽  
pp. 22-35 ◽  
Author(s):  
Suresh D. Desai ◽  
Santosh Kumar ◽  
Robert W. Currie
Keyword(s):  
Polymerase Chain Reaction ◽  
Honey Bee ◽  
Multiple Infections ◽  
Black Queen Cell Virus ◽  
Chain Reaction ◽  
Deformed Wing Virus ◽  
Sacbrood Virus ◽  
Queen Cell ◽  
Polymerase Chain ◽  
Paralysis Virus

AbstractThe occurrence, quantification, and distribution patterns of deformed wing virus (DWV) and sacbrood virus (SBV), (family Iflaviridae); black queen cell virus (BQCV), Israeli acute paralysis virus (IAPV), Kashmir bee virus (KBV), and acute bee paralysis virus (ABPV) (family Dicistroviridae), and chronic bee paralysis virus (CBPV) (unclassified), were characterised in 80 “healthy” honey bee (Apis mellifera Linnaeus; Hymenoptera: Apidae) colonies and 23 “unhealthy” colonies by employing reverse transcription polymerase chain reaction (RT-PCR) for virus identification and quantitative real-time polymerase chain reaction (qPCR) for quantification. All seven viruses were common but the most prevalent viruses were DWV, followed by BQCV and IAPV. For most viruses, prevalence in surviving but unhealthy colonies in spring did not differ from that of healthy baseline colony levels in fall suggesting spring prevalence level would not be a useful metric for diagnosis of factors contributing to colony loss. Sacbrood virus was the only virus that was more prevalent in unhealthy colonies from Manitoba, Canada than in healthy from colonies across Canada but did not differ from healthy colonies within Manitoba. Multiple infections were ubiquitous with a few colonies having simultaneous infection with as many as five viruses. Among the three viruses quantified by qPCR, DWV had the highest relative concentrations in pooled samples of worker bees. Deformed wing virus was the only virus within healthy colonies that differed in fall concentration among provinces and was at high levels in unhealthy colonies. Black queen cell virus was positively correlated with IAPV across all samples. Our study provides the first major baseline study of viruses in Canadian honey bees.

Black queen-cell virus RNA is infectious in honey bee pupae

2002 ◽  
Vol 81 (3) ◽  
pp. 205-206 ◽  
Author(s):  
Mongi Benjeddou ◽  
Neil Leat ◽  
Sean Davison
Keyword(s):  
Honey Bee ◽  
Black Queen Cell Virus ◽  
Virus Rna ◽  
Queen Cell

scholarly journals Home sick: impacts of migratory beekeeping on honey bee (Apis mellifera) pests, pathogens, and colony size

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5812 ◽  
Author(s):  
Samantha A. Alger ◽  
P. Alexander Burnham ◽  
Zachary S. Lamas ◽  
Alison K. Brody ◽  
Leif L. Richardson
Keyword(s):  
Honey Bee ◽  
Pesticide Exposure ◽  
Floral Resources ◽  
Black Queen Cell Virus ◽  
Manipulative Experiment ◽  
Viral Pathogen ◽  
Deformed Wing Virus ◽  
Single Location ◽  
Queen Cell ◽  
Bee Colonies

Honey bees are important pollinators of agricultural crops and the dramatic losses of honey bee colonies have risen to a level of international concern. Potential contributors to such losses include pesticide exposure, lack of floral resources and parasites and pathogens. The damaging effects of all of these may be exacerbated by apicultural practices. To meet the pollination demand of US crops, bees are transported to areas of high pollination demand throughout the year. Compared to stationary colonies, risk of parasitism and infectious disease may be greater for migratory bees than those that remain in a single location, although this has not been experimentally established. Here, we conducted a manipulative experiment to test whether viral pathogen and parasite loads increase as a result of colonies being transported for pollination of a major US crop, California almonds. We also tested if they subsequently transmit those diseases to stationary colonies upon return to their home apiaries. Colonies started with equivalent numbers of bees, however migratory colonies returned with fewer bees compared to stationary colonies and this difference remained one month later. Migratory colonies returned with higher black queen cell virus loads than stationary colonies, but loads were similar between groups one month later. Colonies exposed to migratory bees experienced a greater increase of deformed wing virus prevalence and load compared to the isolated group. The three groups had similar infestations of Varroa mites upon return of the migratory colonies. However, one month later, mite loads in migratory colonies were significantly lower compared to the other groups, possibly because of lower number of host bees. Our study demonstrates that migratory pollination practices has varying health effects for honey bee colonies. Further research is necessary to clarify how migratory pollination practices influence the disease dynamics of honey bee diseases we describe here.

scholarly journals Black queen cell virus and drifting of honey bee workers (Apis mellifera)

2019 ◽  
Vol 58 (5) ◽  
pp. 754-755 ◽  
Author(s):  
Gina Retschnig ◽  
Liv A. Kellermann ◽  
Marion M. Mehmann ◽  
Orlando Yañez ◽  
Pius Winiger ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Black Queen Cell Virus ◽  
Queen Cell ◽  
Honey Bee Workers

scholarly journals Detection of Lotmaria passim, Crithidia mellificae and Replicative Forms of Deformed Wing Virus and Kashmir Bee Virus in the Small Hive Beetle (Aethina tumida)

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 372
Author(s):  
Antonio Nanetti ◽  
James D. Ellis ◽  
Ilaria Cardaio ◽  
Giovanni Cilia
Keyword(s):  
Honey Bee ◽  
Aethina Tumida ◽  
Small Hive Beetle ◽  
Deformed Wing Virus ◽  
Queen Cell ◽  
Kashmir Bee Virus ◽  
Acute Bee Paralysis Virus ◽  
Bee Virus ◽  
Paralysis Virus ◽  
Lotmaria Passim

Knowledge regarding the honey bee pathogens borne by invasive bee pests remains scarce. This investigation aimed to assess the presence in Aethina tumida (small hive beetle, SHB) adults of honey bee pathogens belonging to the following groups: (i) bacteria (Paenibacillus larvae and Melissococcus plutonius), (ii) trypanosomatids (Lotmaria passim and Crithidia mellificae), and (iii) viruses (black queen cell virus, Kashmir bee virus, deformed wing virus, slow paralysis virus, sacbrood virus, Israeli acute paralysis virus, acute bee paralysis virus, chronic bee paralysis virus). Specimens were collected from free-flying colonies in Gainesville (Florida, U.S.A.) in summer 2017. The results of the molecular analysis show the presence of L. passim, C. mellificae, and replicative forms of deformed wing virus (DWV) and Kashmir bee virus (KBV). Replicative forms of KBV have not previously been reported. These results support the hypothesis of pathogen spillover between managed honey bees and the SHB, and these dynamics require further investigation.

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