Cloning of Two Acetylcholinesterase Genes and Analysis of Point Mutations Putatively Associated with Triazophos Resistance in Chilo auricilius (Lepidoptera: Pyralidae)

2015 ◽  
Vol 108 (3) ◽  
pp. 1289-1297 ◽  
Author(s):  
G.-H. Luo ◽  
X.-H. Li ◽  
Z.-C. Zhang ◽  
B.-S. Liu ◽  
S.-J. Huang ◽  
...  
Keyword(s):  
Point Mutations ◽  
Lepidoptera Pyralidae ◽  
Chilo Auricilius

scholarly journals Uji Daya Parasitasi Trichogramma australicum Gir. (Hymenoptera : Trichogrammatidae) Terhadap Telur Corcyra cephalonica Staint (Lepidoptera : Pyralidae) Dan Telur Chilo aurilicius Dudg (Lepidoptera : Pyralidae) Di Laboratorium

2012 ◽  
Vol 14 (1) ◽  
pp. 42
Author(s):  
Mochamad Hadi
Keyword(s):  
Corcyra Cephalonica ◽  
Key Word ◽  
Lepidoptera Pyralidae ◽  
Chilo Auricilius

Penelitian mengenai daya parasitasi Trichogramma australicum Gir. terhadap inang alami dan inang pengganti ini bertujuan untuk mengetahui apakah T. australicum setelah bertahun-tahun dipelihara dengan menggunakan inang pengganti masih mampu memarasiti inang alaminya secara efektif. Penelitian menggunakan Rancangan Acak Lengkap dengan lima kali ulangan. Perlakuan adalah pemarasitan telur penggerek batang tebu Chilo auricilius Dudg dan telur ngengat beras Corcyra cephalonica Staint. Perhitungan data dengan parameter jumlah pemarasitan, penetasan parasitoid, dan kemampuan pemarasitan per individu parasitoid betina. Pemeliharaan T. australicum dengan menggunakan inang pengganti tidak mempengaruhi kemampuan pemarasitan terhadap inang alaminya. Parasitoid T. australicum masih memiliki potensi besar untuk digunakan sebagai agen pengendali hayati penggerek batang tebu, khususnya penggerek batang tebu berkilat C. auricilius. Rata-rata pemarasitan T. australicum terhadap telur C. cephalonica dan telur C. auricilius masing-masing sebesar 96.14% dan 95.0%. Penetasan parasitoid dari telur C. cephalonica dan telur C. auricilius masing-masing sebesar 97.13% dan 96.21%. Daya parasitasi per individu parasitoid betina terhadap telur C. cephalonica dan telur C. auricilius masing-masing sebesar 4.80 dan 4.75. Hasil analisis statistik terhadap semua uji menunjukkan tidak ada beda nyata antara kedua perlakuan.   Key word : daya parasitasi, Trichogramma australicum Gir. Chilo auricilius Dudg, Corcyra cephalonica Staint

A Novel Mutation Glyl672→Arg in Type 2A and a Homozygous Mutation in Type 2B von Willebrand Disease

1996 ◽  
Vol 76 (02) ◽  
pp. 253-257 ◽  
Author(s):  
Takeshi Hagiwara ◽  
Hiroshi Inaba ◽  
Shinichi Yoshida ◽  
Keiko Nagaizumi ◽  
Morio Arai ◽  
...  
Keyword(s):  
Missense Mutation ◽  
Novel Mutation ◽  
Point Mutations ◽  
Japanese Patients ◽  
Von Willebrand Disease ◽  
Homozygous Mutation ◽  
Missense Mutations ◽  
Reaction Products ◽  
Type 3 ◽  
Von Willebrand

SummaryGenetic materials from 16 unrelated Japanese patients with von Willebrand disease (vWD) were analyzed for mutations. Exon 28 of the von Willebrand factor (vWF) gene, where point mutations have been found most frequent, was screened by various restriction-enzyme analyses. Six patients were observed to have abnormal restriction patterns. By sequence analyses of the polymerase chain-reaction products, we identified a homozygous R1308C missense mutation in a patient with type 2B vWD; R1597W, R1597Q, G1609R and G1672R missense mutations in five patients with type 2A; and a G1659ter nonsense mutation in a patient with type 3 vWD. The G1672R was a novel missense mutation of the carboxyl-terminal end of the A2 domain. In addition, we detected an A/C polymorphism at nucleotide 4915 with HaeIII. There was no particular linkage disequilibrium of the A/C polymorphism, either with the G/A polymorphism at nucleotide 4391 detected with Hphl or with the C/T at 4891 detected with BstEll.

PREVALENCE OF COMMON MITOCHONDRIAL POINT MUTATIONS IN AUTISM

2006 ◽  
Vol 37 (S 1) ◽  
Author(s):  
FJ Serajee ◽  
H Zhong ◽  
AHMM Huq
Keyword(s):  
Point Mutations

Cupin Variants as Macromolecular Ligand Library for Stereoselective Michael Addition of Nitroalkanes

2019 ◽  
Author(s):  
Nobutaka Fujieda ◽  
Miho Yuasa ◽  
Yosuke Nishikawa ◽  
Genji Kurisu ◽  
Shinobu Itoh ◽  
...  
Keyword(s):  
Michael Addition ◽  
In Silico ◽  
Addition Reaction ◽  
Single Point ◽  
Point Mutations ◽  
Unsaturated Ketone ◽  
Michael Addition Reaction ◽  
Beta Barrel ◽  
Cupin Superfamily ◽  
Single Point Mutations

Cupin superfamily proteins (TM1459) work as a macromolecular ligand framework with a double-stranded beta-barrel structure ligating to a Cu ion through histidine side chains. Variegating the first coordination sphere of TM1459 revealed that H52A and H54A/H58A mutants effectively catalyzed the diastereo- and enantio-selective Michael addition reaction of nitroalkanes to an α,β-unsaturated ketone. Moreover, in silico substrate docking signified C106N and F104W single-point mutations, which inverted the diastereoselectivity of H52A and further improved the stereoselectivity of H54A/H58A, respectively.

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