Detection of Two Biotypes of Liriomyza chinensis (Diptera: Agromyzidae) in Japan

2021 ◽  
Author(s):  
Susumu Tokumaru ◽  
Ryuji Uesugi ◽  
Chihiro Urairi ◽  
Shingo Toyoshima ◽  
Yumi Aoki ◽  
...  
Keyword(s):  
Welsh Onion ◽  
Inner Surface ◽  
Biotype B ◽  
Haplotype A ◽  
Mtcoi Gene

Abstract The larva of stone leek leafminer, Liriomyza chinensis (Kato), is known to infest alternately just below the epidermis and inner surface of hollow cylindrical leaves of allium crops, resulting in the formation of discontinuous linear mines (mine form: discontinuous). However, after the fall of 2016, a novel mine form of the same species (mine form: continuous) was detected in Welsh onion fields of Kyoto Prefecture, Japan. We hypothesized that these mine forms were associated with flies having different genetic backgrounds; hence, we compared the mine forms and the partial mtCOI gene of flies collected from Welsh onion fields from 2018 to 2019. The results demonstrated that the flies that emerged from different mine forms could be classified into two haplogroups, i.e., flies displaying a discontinuous mine form were of haplogroup A, whereas those that displayed continuous mines were of haplotype B. Additionally, using populations of these flies reared in the laboratory, we confirmed that the mine form of the larvae of haplotype A on Welsh onions was discontinuous, whereas that of haplotype B was continuous. We named the population that exhibited a discontinuous mine form as biotype A and the population displaying a continuous mine form as biotype B.

A SEM and Light Microscope Study of the Epidermal Leaf Structures of the Carnivorous Plant, Sarracenia purpurea, L.

1971 ◽  
Vol 29 ◽  
pp. 358-359
Author(s):  
B. J. Panessa ◽  
J. F. Gennaro
Keyword(s):  
Methylene Blue ◽  
Toluidine Blue ◽  
Outer Surface ◽  
Microscope Study ◽  
Light Microscope ◽  
Carnivorous Plant ◽  
Sarracenia Purpurea ◽  
Inner Surface

Tissue from the hood and sarcophagus regions were fixed in 6% glutaraldehyde in 1 M.cacodylate buffer and washed in buffer. Tissue for SEM was partially dried, attached to aluminium targets with silver conducting paint, carbon-gold coated(100-500Å), and examined in a Kent Cambridge Stereoscan S4. Tissue for the light microscope was post fixed in 1% aqueous OsO4, dehydrated in acetone (4°C), embedded in Epon 812 and sectioned at ½u on a Sorvall MT 2 ultramicrotome. Cross and longitudinal sections were cut and stained with PAS, 0.5% toluidine blue and 1% azure II-methylene blue. Measurements were made from both SEM and Light micrographs.The tissue had two structurally distinct surfaces, an outer surface with small (225-500 µ) pubescent hairs (12/mm2), numerous stoma (77/mm2), and nectar glands(8/mm2); and an inner surface with large (784-1000 µ)stiff hairs(4/mm2), fewer stoma (46/mm2) and larger, more complex glands(16/mm2), presumably of a digestive nature.

Gastric Ulcers Produced by Cisplatin

1981 ◽  
Vol 39 ◽  
pp. 582-583
Author(s):  
S.K. Aggarwal ◽  
J. San Antonio
Keyword(s):  
Electron Microscopy ◽  
Single Dose ◽  
Side Effects ◽  
Antitumor Agent ◽  
Gastric Ulcers ◽  
Enzyme Cytochemistry ◽  
Intestinal Toxicity ◽  
Ovarian Cancers ◽  
Inner Surface

Cisplatin (cis-dichlorodiammineplatinum(II)) a potent antitumor agent is now available for the treatment of testicular and ovarian cancers. It is however, not free from its serious side effects including nephrotoxicity, gastro intestinal toxicity, myelosuppression, and ototoxicity. Here we now report that the drug produces peculiar bloating of the stomach in rats and induces acute ulceration.Wistar-derived rats weighing 200-250 g were administered cisplatin(9 mg/kg) ip as a single dose in 0.15 M NaCl. After 3 days the animals were sacrificed by decapitation. The stomachs were removed, the contents analyzed for pepsin and acidity. The inner surface was examined with a dissecting microscope after a moderate stretching for ulcers. Affected areas were fixed and processed for routine electron microscopy and enzyme cytochemistry.The drug treated animals kept on food and water consistently showed bloating and lesions (Fig. 1) with a frequency of 6-70 ulcers in the rumen section of the stomachs.

Probabilistic evaluation of detection capability of eddy current testing to inspect pitting on a stainless steel clad using multiple signal features

2020 ◽  
Vol 64 (1-4) ◽  
pp. 47-55
Author(s):  
Takuma Tomizawa ◽  
Haicheng Song ◽  
Noritaka Yusa
Keyword(s):  
Stainless Steel ◽  
Eddy Current ◽  
Pressure Vessels ◽  
Eddy Current Testing ◽  
Probability Of Detection ◽  
Current Testing ◽  
Multiple Signal ◽  
Signal Features ◽  
Inner Surface ◽  
Drill Holes

This study proposes a probability of detection (POD) model to quantitatively evaluate the capability of eddy current testing to detect flaws on the inner surface of pressure vessels cladded by stainless steel and in the presence of high noise level. Welded plate samples with drill holes were prepared to simulate corrosion that typically appears on the inner surface of large-scale pressure vessels. The signals generated by the drill holes and the noise caused by the weld were examined using eddy current testing. A hit/miss-based POD model with multiple flaw parameters and multiple signal features was proposed to analyze the measured signals. It is shown that the proposed model is able to more reasonably characterize the detectability of eddy current signals compared to conventional models that consider a single signal feature.

Intensification of Open Gutters Operation

2020 ◽  
pp. 66-70
Keyword(s):  
Water Flow ◽  
Vortex Formation ◽  
Hydraulic Testing ◽  
Polymer Materials ◽  
Bottom Surface ◽  
Artificial Roughness ◽  
Transport Capacity ◽  
Corrugated Surfaces ◽  
Inner Surface ◽  
Dispersed Inclusions

The intensification of the work of open gutter by applying textured shells to their bottom surface, forming an artificial roughness, is considered. It is shown that the presence of corrugated surfaces contributes to vortex formation during water flow and improves the separation and transportation of mineral impurities previously dropped into the bottom of the gutters. The implementation of operations to improve the structure of the gutters is possible during the repair and restoration works with the use of modern polymer materials. The design of a small-sized hydraulic stand, which makes it possible to study the transport capacity of flows containing solid inclusions, is presented. The method of research is hydraulic testing, accompanied by the use of chiaroscuro effect, as well as photo and film equipment. The optimal structure of the inner surface of the gutters and pipes providing vortex formation, which will improve the ability of the flow to carry out and transport foreign dispersed inclusions (sand) of different granulometric compositions, is determined.

scholarly journals Phytochemical Analysis and In Vitro Effects of Allium fistulosum L. and Allium sativum L. Extracts on Human Normal and Tumor Cell Lines: A Comparative Study

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 574
Author(s):  
Adrian Bogdan Țigu ◽  
Cristian Silviu Moldovan ◽  
Vlad-Alexandru Toma ◽  
Anca Daniela Farcaș ◽  
Augustin Cătălin Moț ◽  
...  
Keyword(s):  
Cell Line ◽  
Allium Sativum ◽  
Allium Fistulosum ◽  
Tumor Cell Lines ◽  
Sensitive Cell ◽  
Sensitive Cell Line ◽  
Welsh Onion ◽  
Normal Cells ◽  
Ic50 Value ◽  
High Concentrations

Allium sativum L. (garlic bulbs) and Allium fistulosum L. (Welsh onion leaves) showed quantitative differences of identified compounds: allicin and alliin (380 µg/mL and 1410 µg/mL in garlic; 20 µg/mL and 145 µg/mL in Welsh onion), and the phenolic compounds (chlorogenic acid, p-coumaric acid, ferulic acid, gentisic acid, 4-hydroxybenzoic acid, kaempferol, isoquercitrin, quercitrin, quercetin, and rutin). The chemical composition determined the inhibitory activity of Allium extracts in a dose-dependent manner, on human normal cells (BJ-IC50 0.8841% garlic/0.2433% Welsh onion and HaCaT-IC50 1.086% garlic/0.6197% Welsh onion) and tumor cells (DLD-1-IC50 5.482%/2.124%; MDA-MB-231-IC50 6.375%/2.464%; MCF-7-IC50 6.131%/3.353%; and SK-MES-1-IC50 4.651%/5.819%). At high concentrations, the cytotoxic activity of each extract, on normal cells, was confirmed by: the 50% of the growth inhibition concentration (IC50) value, the cell death induced by necrosis, and biochemical determination of LDH, catalase, and Caspase-3. The four tumor cell lines treated with high concentrations (10%, 5%, 2.5%, and 1.25%) of garlic extract showed different sensibility, appreciated on the base of IC50 value for the most sensitive cell line (SK-MES-1), and the less sensitive (MDA-MB-231) cell line. The high concentrations of Welsh onion extract (5%, 2.5%, and 1.25%) induced pH changes in the culture medium and SK-MES-1 being the less sensitive cell line.

Sign in / Sign up

Export Citation Format

Share Document