scholarly journals Association Between Susceptibility of Thrips palmi to Spinetoram and Frequency of G275E Mutation Provides Basis for Molecular Quantification of Field-Evolved Resistance

2021 ◽  
Author(s):  
Pan Shi ◽  
Shao-Kun Guo ◽  
Yong-Fu Gao ◽  
Jin-Cui Chen ◽  
Ya-Jun Gong ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Nicotinic Acetylcholine Receptor ◽  
Mutation Frequency ◽  
Diagnostic Method ◽  
Field Resistance ◽  
Molecular Diagnostic ◽  
Nicotinic Acetylcholine ◽  
Thrips Palmi ◽  
Melon Thrips ◽  
Molecular Diagnostic Method

Abstract Putative mechanisms underlying spinosyn resistance have been identified in controlled studies on many species; however, mechanisms underlying field-evolved resistance and the development of a molecular diagnostic method for monitoring field resistance have lagged behind. Here, we examined levels of resistance of melon thrips, Thrips palmi Karny (Thysanoptera:Thripidae), to spinetoram as well as target site mutations in field populations across China to identify potential mechanisms and useful molecular markers for diagnostic and quantifying purposes. In resistant populations, we identified the G275E mutation, which has previously been linked to spinosyns resistance, and F314V mutation, both located in the α6 subunit of the nicotinic acetylcholine receptor. There was a strong correlation between levels of spinetoram resistance and allele frequency of G275E mutation in field-collected populations (r2 = 0.84) and those reared under laboratory conditions for two to five generations (r2 = 0.91). LC50 ranged from 0.12 to 0.66 mg/liter in populations without G275E mutation, whereas it ranged from 33.12 to 39.91 mg/liter in most populations with a G275E mutation frequency more than 90%. Our results indicate that the field-evolved resistance of T. palmi to spinetoram in China is mainly conferred by the G275E mutation. The frequency of the G275E mutation provides a useful diagnostic for quantifying resistance levels in field populations of T. palmi.

scholarly journals Why cannot a β-lactamase gene be detected using an efficient molecular diagnostic method?

2016 ◽  
Vol 32 (5) ◽  
Author(s):  
Sang Hee Lee ◽  
Kwang Seung Park ◽  
Jung Hun Lee ◽  
Moonhee Park ◽  
Asad Mustafa Karim
Keyword(s):  
Diagnostic Method ◽  
Molecular Diagnostic ◽  
Lactamase Gene ◽  
Molecular Diagnostic Method

A rapid molecular diagnostic method for spinal muscular atrophy

2020 ◽  
pp. 1-4
Author(s):  
Kai-Chen Wang ◽  
Chiao-Yuan Fang ◽  
Chi-Chang Chang ◽  
Chien-Kuan Chiang ◽  
Yi-Wen Chen
Keyword(s):  
Spinal Muscular Atrophy ◽  
Diagnostic Method ◽  
Muscular Atrophy ◽  
Molecular Diagnostic ◽  
Molecular Diagnostic Method

scholarly journals MO37-1 Identification and evaluation of a serum microRNA molecular diagnostic method for colorectal polyp patients

2021 ◽  
Vol 32 ◽  
pp. S322
Author(s):  
Lui Ng ◽  
Ryan W.Y. Sin ◽  
Oswens S.H. Lo ◽  
Carlos K.H. Wong ◽  
Cindy L.K. Lam ◽  
...  
Keyword(s):  
Diagnostic Method ◽  
Colorectal Polyp ◽  
Molecular Diagnostic ◽  
Serum Microrna ◽  
Molecular Diagnostic Method

scholarly journals Improving RT-LAMP Detection of SARS-CoV-2 RNA through Primer Set Selection and Combination

2021 ◽  
Author(s):  
Nathan Tanner ◽  
Yinhua Zhang
Keyword(s):  
Nucleic Acid ◽  
Diagnostic Method ◽  
High Sensitivity ◽  
Sensitivity Enhancement ◽  
Molecular Diagnostic ◽  
Nucleic Acid Testing ◽  
Lamp Primer ◽  
Body Of Knowledge ◽  
Primer Sets ◽  
Molecular Diagnostic Method

Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has emerged as a viable molecular diagnostic method to expand the breadth and reach of nucleic acid testing, particularly for SARS-CoV-2 detection and surveillance. While rapidly growing in prominence, RT-LAMP remains a relatively new method compared to the standard RT-qPCR, and contribution to our body of knowledge on designing LAMP primer sets and assays can have significant impact on its utility and adoption. Here we evaluate 18 LAMP primer sets for SARS-CoV-2, comparing speed and sensitivity with different LAMP formulations and conditions across more than 5,000 RT-LAMP reactions and identifying several primer sets with similar high sensitivity for different SARS-CoV-2 gene targets. Significantly we observe a consistent sensitivity enhancement by combining primer sets for different targets, confirming and building on earlier work to create a simple, general approach to building better and more sensitive RT-LAMP assays.

scholarly journals Effectiveness of CRISPR based tools as alternative methods for diagnosing COVID-19

2021 ◽  
Vol 8 (3) ◽  
pp. 150-152
Author(s):  
Shefali Mehta ◽  
Aditi Mehta
Keyword(s):  
Diagnostic Method ◽  
Diagnostic Methods ◽  
Alternative Methods ◽  
Molecular Diagnostic ◽  
Epigenome Editing ◽  
Adaptive Immune ◽  
Efficiency And Effectiveness ◽  
Associated Proteins ◽  
Rapid Pace ◽  
Molecular Diagnostic Method

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas (CRISPR – Associated Proteins) systems are prokaryotic adaptive immune mechanisms that are used for cleaving the invading nucleic acids in nature. Due to this reason, the CRISPR-based tools are used in numerous applications like genome and transcriptome engineering, gene therapy, epigenome editing and many others. In addition, the issue of errors in these tests is also increasing at a very rapid pace, due to which their reliability and efficiency and effectiveness are getting severely hampered. Therefore, it may not be wrong to say that there is a still a lot more room for improvement and development to enable, widespread, rapid, and scalable testing of the COVID-19 virus. In this regard, the RT-qPCR tests can be used as a routine molecular diagnostic method for detecting any COVID-19 symptoms. However, there are various limitations that mar the use and implementation of the RT-qPCR tests. But a few of these shortcomings can be overcome by using alternative molecular diagnostic methods like the CRISPR based tests.

scholarly journals Development and Evaluation of a Molecular Diagnostic Method for Rapid Detection of Histoplasma capsulatum var. farciminosum, the Causative Agent of Epizootic Lymphangitis, in Equine Clinical Samples

2016 ◽  
Vol 54 (12) ◽  
pp. 2990-2999
Author(s):  
C. E. Scantlebury ◽  
G. L. Pinchbeck ◽  
P. Loughnane ◽  
N. Aklilu ◽  
T. Ashine ◽  
...  
Keyword(s):  
Causative Agent ◽  
Nested Pcr ◽  
Diagnostic Method ◽  
Histoplasma Capsulatum ◽  
Clinical Signs ◽  
Molecular Diagnostic ◽  
Clinical Samples ◽  
Blood Samples ◽  
Content Type ◽  
Molecular Diagnostic Method

Histoplasma capsulatumvar.farciminosum, the causative agent of epizootic lymphangitis (EZL), is endemic in parts of Africa. Diagnosis based on clinical signs and microscopy lacks specificity and is a barrier to further understanding this neglected disease. Here, a nested PCR method targeting the internal transcribed spacer (ITS) region of the rRNA operon was validated for application to equine clinical samples. Twenty-nine horses with signs of EZL from different climatic regions of Ethiopia were clinically examined. Blood samples and aspirates of pus from cutaneous nodules were taken, along with blood from a further 20 horses with no cutaneous EZL lesions. Among the 29 horses with suspected cases of EZL,H. capsulatumvar.farciminosumwas confirmed by extraction of DNA from pus and blood samples from 25 and 17 horses, respectively. Positive PCR results were also obtained with heat-inactivated pus (24 horses) and blood (23 horses) spotted onto Whatman FTA cards. Two positive results were obtained among blood samples from 20 horses that did not exhibit clinical signs of EZL. These are the first reports of the direct detection ofH. capsulatumvar.farciminosumin equine blood and at high frequency among horses exhibiting cutaneous lesions. The nested PCR outperformed conventional microscopic diagnosis, as characteristic yeast cells could be observed only in 14 pus samples. The presence ofH. capsulatumvar.farciminosumDNA was confirmed by sequencing the cloned PCR products, and while alignment of the ITS amplicons showed very little sequence variation, there was preliminary single nucleotide polymorphism-based evidence for the existence of two subgroups ofH. capsulatumvar.farciminosum. This molecular diagnostic method now permits investigation of the epidemiology of EZL.

scholarly journals LACK OF SPECIFICITY OF THE MOLECULAR DIAGNOSTIC METHOD FOR IDENTIFICATION OF APHANOMYCES ASTACI

2007 ◽  
pp. 17-24 ◽  
Author(s):  
I. BALLESTEROS ◽  
M. P. MARTÍN ◽  
L. CERENIUS ◽  
K. SÖDERHÄLL ◽  
M. T. TELLERÍA ◽  
...  
Keyword(s):  
Diagnostic Method ◽  
Molecular Diagnostic ◽  
Aphanomyces Astaci ◽  
Molecular Diagnostic Method
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