Effect of Insect Damage to Maize Ears, with Special Reference to Mussidia nigrivenella (Lepidoptera: Pyralidae), on Aspergillus flavus (Deuteromycetes: Monoliales) Infection and Aflatoxin Production in Maize Before Harvest in the Republic of Benin

1998 ◽  
Vol 91 (2) ◽  
pp. 433-438 ◽  
Author(s):  
M. SÉTamou ◽  
K. F. Cardwell ◽  
F. Schulthess ◽  
K. Hell
Toxins ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 687
Author(s):  
Vanja Vlajkov ◽  
Mila Grahovac ◽  
Dragana Budakov ◽  
Marta Loc ◽  
Ivana Pajčin ◽  
...  

Maize is one of the leading export products in the Republic of Serbia. As a country where economic development depends on agriculture, maize production plays a critical role as a crop of strategic importance. Potential aflatoxin contamination of maize poses a risk to food and feed safety and tremendous economic losses. No aflatoxin contamination of maize samples harvested in 2019 and 2020 in different localities in the Republic of Serbia was detected by the Enzyme-Linked Immunosorbent Assay (ELISA) test and High-Performance Liquid Chromatography (HPLC) method. On the other hand, the Cluster Amplification Patterns (CAP) analyses of the isolated Aspergillus flavus strains from 2019 maize samples confirmed the presence of key biosynthesis genes responsible for aflatoxin production. Artificial inoculation and subsequent HPLC analysis of the inoculated maize samples confirmed the high capacity of the A. flavus strains for aflatoxin production, pointing to a high risk of contamination under favorable conditions. Prevention of aflatoxin contamination is primarily based on A. flavus control, where biocontrol agents play a significant role as sustainable disease management tools. In this study, antagonistic activity screening of the novel strains belonging to the Bacillus genus indicated superior suppression of A. flavus strains by two Bacillus strains isolated from the rhizosphere of Phaseolus vulgaris.


Author(s):  
Kpatinde Talagbe Aime ◽  
A. H. Abdul Wahab ◽  
K. S. J. Fadina ◽  
W. K. A. Oting ◽  
D. B. O. Sinatoko

2020 ◽  
Vol 64 (5) ◽  
pp. 566-575
Author(s):  
Degbegnon Kevin Marie ◽  

Статья посвящена исследованию и разработке основных положений зонирования территорий насе- ленных пунктов Республики Бенин. Разработаны схемы зонирования территорий, определяющие вид использования территорий и устанавливающие ограничения, функциональное назначение и интенсив- ность использования каждой территориальной зоны. Рассмотрены нормативные правовые акты орга- нов местного самоуправления, регулирующие использование и изменение объектов недвижимости.


2019 ◽  
Vol 63 (1) ◽  
pp. 90-98
Author(s):  
Degbegnon Kevin Marie ◽  
◽  
Ilyushina T.V. ◽  
Degbegnon Owiwosson Pierrette Aurelle ◽  
◽  
...  

2020 ◽  
Vol 6 (4) ◽  
pp. 383
Author(s):  
Premila Narayana Achar ◽  
Pham Quyen ◽  
Emmanuel C. Adukwu ◽  
Abhishek Sharma ◽  
Huggins Zephaniah Msimanga ◽  
...  

Aspergillus species are known to cause damage to food crops and are associated with opportunistic infections in humans. In the United States, significant losses have been reported in peanut production due to contamination caused by the Aspergillus species. This study evaluated the antifungal effect and anti-aflatoxin activity of selected plant-based essential oils (EOs) against Aspergillus flavus in contaminated peanuts, Tifguard, runner type variety. All fifteen essential oils, tested by the poisoned food technique, inhibited the growth of A. flavus at concentrations ranging between 125 and 4000 ppm. The most effective oils with total clearance of the A. flavus on agar were clove (500 ppm), thyme (1000 ppm), lemongrass, and cinnamon (2000 ppm) EOs. The gas chromatography-mass spectrometry (GC-MS) analysis of clove EO revealed eugenol (83.25%) as a major bioactive constituent. An electron microscopy study revealed that clove EO at 500 ppm caused noticeable morphological and ultrastructural alterations of the somatic and reproductive structures. Using both the ammonia vapor (AV) and coconut milk agar (CMA) methods, we not only detected the presence of an aflatoxigenic form of A. flavus in our contaminated peanuts, but we also observed that aflatoxin production was inhibited by clove EO at concentrations between 500 and 2000 ppm. In addition, we established a correlation between the concentration of clove EO and AFB1 production by reverse-phase high-performance liquid chromatography (HPLC). We demonstrate in our study that clove oil could be a promising natural fungicide for an effective bio-control, non-toxic bio-preservative, and an eco-friendly alternative to synthetic additives against A. flavus in Georgia peanuts.


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